List of non-binary characters in animation

This is a list of non-binary characters in animation that either self-identify as non-binary or have been identified by outside parties to be of the non-binary gender, agender, bigender, genderfluid, genderqueer, as well as characters of any third gender. Listed characters are either recurring characters, cameos, guest stars, or one-off characters in animated series, but not animated films. This article also includes characters in Japanese animation, otherwise known as anime.
For fictional characters in other parts of the LGBTQ community, see the lists of gay, trans, lesbian, bisexual, pansexual, asexual, and intersex characters.
The names are organized alphabetically by surname, or by a single name if the character does not have a surname. If more than two characters are in one entry, the last name of the first character is used. These characters are organized by their identities within the non-binary umbrella of identities, rather than by years, as corresponding lists of lesbian, bisexual and gay animated characters, do.

Non-binary and genderqueer

Character	Show title	Portrayed by	Duration	Notes
Admiral Akers	The Bravest Knight	Asia Kate Dillon	2019–present	A seafaring character who appears in the episode "Cedric & Pirate Cove" and corrects another character when they assume the wrong pronouns, saying "I don’t go by ‘he;’ I go by they. Some are ‘he’ and some are ‘she,’ but neither one will do for me."
Alex	Primos	Blu del Barrio	2024	Alex is a non-binary person. who is dating Lita Perez, who is Tater's eldest cousin and is bisexual. The episode Alex and Lita appeared in was nominated for a GLAAD Media Award for Outstanding Kids & Family Programming or Film – Animated.
Alien King	Summer Camp Island	Sam Lavagnino	2018–2023	King of a planet and married to an alien named Puddle.
Alien King	Summer Camp Island	Dylan Alvarado	2018–2023	King of a planet and married to an alien named Puddle.
Ryuji "Yuka" Ayukawa	Blue Period	Yumiri Hanamori	2021	Yuka is one of Yatora Yaguchi's friends. They wear both male and female school uniforms. In the anime, they are the source of mockery of other students who misgender and deadnamed them, while in the manga no one seems to pay them any mind. Yuka is popular in class, called "Yuka-chan" by some, and is on good terms with Yaguchi.
D'eon de Beaumont/Lia de Beaumont	Le Chevalier D'Eon	Yūki Tai	2006–2007	Lia de Beaumont is killed and her brother D'eon de Beaumont seeks her murderers, and her spirit begins to inhabit his body whenever his life is in danger. This character is based on the identity that real-life cross-dresser, Chevalier d'Eon claimed in a 1756 mission to Russia.
D'eon de Beaumont/Lia de Beaumont	Le Chevalier D'Eon	Akio Nojima	2006–2007	Lia de Beaumont is killed and her brother D'eon de Beaumont seeks her murderers, and her spirit begins to inhabit his body whenever his life is in danger. This character is based on the identity that real-life cross-dresser, Chevalier d'Eon claimed in a 1756 mission to Russia.
D'eon de Beaumont/Lia de Beaumont	Le Chevalier D'Eon	Risa Mizuno	2006–2007	Lia de Beaumont is killed and her brother D'eon de Beaumont seeks her murderers, and her spirit begins to inhabit his body whenever his life is in danger. This character is based on the identity that real-life cross-dresser, Chevalier d'Eon claimed in a 1756 mission to Russia.
Asher Berdacs	Kipo and the Age of Wonderbeasts	River Butcher	2020	When asked by a fan about the gender of Asher, series creator "Rad" Sechrist said that Asher is non-binary and uses singular they pronouns, which was later confirmed by Bill Wolkoff, co-screenwriter of Kipo.
Blaine	Adventure Time: Distant Lands	Bex Taylor-Klaus	2023	Blaine is a young cyclops and a classmate of the character Peppermint Butler. Blaine is referred to with the singular they pronoun in the episode "Distant Lands". Moreover, the official Adventure Time Tumblr account posted concept art of Blaine, including notes referring to them with such language.
Box	We Baby Bears	Amari McCoy	2022–present	Box, the Bears' friend is non-binary and uses they/them pronouns.
Courtney Cahatel	Dead End: Paranormal Park	Emily Osment	2022	Series creator Hamish Steele said that Courtney is not "aware of gender in any way" and cannot be misgendered, noting that all pronouns were used in production. He added that he uses they/them for Courtney, but that Netflix persuaded them to use the pronouns of the person cast for Courtney's voice role. Emily Osment was cast, so Courtney is referred to with she/her pronouns.
Double Trouble	She-Ra and the Princesses of Power	Jacob Tobia	2018–2020	Showrunner ND Stevenson described them at New York Comic Con 2019 as a "nonbinary shapeshifting mercenary". They are voiced by Jacob Tobia, a non-binary person. Double Trouble reappears for brief periods in the show's final season, posing as "Peekablue," a male prince.
Parker J. Cloud	Middlemost Post	Becky Robinson	2021	Parker J. Cloud is an anthropomorphic cloud who delivers mail. Parker is non-binary and uses they/them pronouns. Middlemost Post is the first Nickelodeon series to feature a non-binary lead protagonist.
Sonnie Dracula	Mighty MonsterWheelies	Cal Brady	2024–present	A Dracula motorcycle/helicopter combo. Sonnie is non-binary and uses they/them pronouns. Sonnie Dracula is voiced by transgender actress Cal Brady.
Odee Elliott	Madagascar: A Little Wild	Iris Menas	2021–2022	An okapi, voiced by Iris Menas, who first appears in the season 3 Pride-themed episode "Whatever Floats Your Float," with none of the floats seeming right for Odee, and then sings a song titled "Be Proud" about being proud of your identity. GLAAD consulted on the episode and menas said the episode resonated with hir.
Envy	Fullmetal Alchemist	Mayumi Yamaguchi	2003–2004	Envy is a shapeshifting homunculus without a specific gender. While they are usually referred to in general neutral pronouns in the Japanese version, in both anime they are usually referred to as he, indicate in episodes like "Death". They are normally seen to have the appearance of an 'androgynous' female.
Envy	Fullmetal Alchemist: Brotherhood	Minami Takayama	2009–2010	Envy is a shapeshifting homunculus without a specific gender. While they are usually referred to in general neutral pronouns in the Japanese version, in both anime they are usually referred to as he, indicate in episodes like "Death". They are normally seen to have the appearance of an 'androgynous' female.
Fred	Ridley Jones	Iris Menas	2021–2023	Fred is a non-binary bison who prefers they/them pronouns and is the first non-binary character in a Netflix kids series.
Garnet	Steven Universe	Estelle	2013–2020	Ruby and Sapphire are two sexless but feminine-presenting members of the Crystal Gems who have a romantic relationship with each other, and stay permanently fused to form Garnet. In July 2015, the co-executive producer, Ian Jones-Quartey, has confirmed that, according to human standards and terminology, calling Ruby and Sapphire non-binary, feminine-presenting lesbians would be "a fair assessment". On July 6, 2018, the episode "Reunited" aired, in which Ruby and Sapphire get married, kiss, and fuse into Garnet, after Ruby proposed to Sapphire in a previous episode, "The Question". Series creator Rebecca Sugar has also said that "the Gems are all non-binary women," which includes Garnet, and her friends, Amethyst and Pearl.
Garnet	Steven Universe Future	Estelle	2013–2020	Ruby and Sapphire are two sexless but feminine-presenting members of the Crystal Gems who have a romantic relationship with each other, and stay permanently fused to form Garnet. In July 2015, the co-executive producer, Ian Jones-Quartey, has confirmed that, according to human standards and terminology, calling Ruby and Sapphire non-binary, feminine-presenting lesbians would be "a fair assessment". On July 6, 2018, the episode "Reunited" aired, in which Ruby and Sapphire get married, kiss, and fuse into Garnet, after Ruby proposed to Sapphire in a previous episode, "The Question". Series creator Rebecca Sugar has also said that "the Gems are all non-binary women," which includes Garnet, and her friends, Amethyst and Pearl.
Gregg	OK K.O.! Let's Be Heroes	Ian Jones-Quartey	2017–2019	Gregg is a minor character in the series, usually loitering around the plaza with Red Action and Drupe. Series creator Ian Jones-Quartey confirmed them as non-binary on Twitter.
Violet Harper	Young Justice	Zehra Fazal	2010–2022	Violet Harper, also known as Halo, is the soul of a sentient technology known as a Mother Box that entered the body of Gabrielle Daou. In spite of Halo's outward appearance, the character does not identify as male or female as defined in Earth language. In the episode "Nautical Twilight", Halo self-identifies as non-binary, adding that the singular they pronoun suits them best, although they are unbothered by being called "she". Rocket and the New Gods used feminine pronouns for Halo in the episode "Forbidden Secrets of Civilizations Past!" though this was a writing mistake according to Greg Weisman.
Winn Harper	The Fairly OddParents: A New Wish	Iris Menas	2024	A cool, purple-haired elementary schooler fond of skateboarding, and one of Hazel Wells' friends. Winn is non-binary and uses they/them pronouns, with one character saying in the episode "Fly," "That's Winn. They're cool, you don't stand a chance." Winn is voiced by non-binary actor Iris Menas.
Hyena	Give Me Three Tickets		2020	In this Chilean adult animation produced by Pájaro Estudio, which was presented at the Animation! section of the Argentine film festival, "Ventana Sur", there is a party-loving non-binary hyena, a posh albino giraffe, and monkey who is a liberal shaman, with all three sharing everything. This includes their "love lives". Things change when one of these characters tries to bring a new person into their relationship.
Emporio Ivankov	One Piece	Norio Imamura	1999–present	Ivankov is the self-declared queen of an island populated by okama, having powers that allow him to shift between male and female forms, while also changing genders of other individuals. Also known as Iva, and has been involved in various battles with villains over the course of the show.
Emporio Ivankov	One Piece	Mitsuo Iwata	1999–present	Ivankov is the self-declared queen of an island populated by okama, having powers that allow him to shift between male and female forms, while also changing genders of other individuals. Also known as Iva, and has been involved in various battles with villains over the course of the show.
Jae	Supernatural Academy	Ali J. Eisner	2022	Jae is a Faerie student who goes to Supernatural Academy. They are non-binary and prefer they/them pronouns. Jae is voiced by transgender and non-binary actor Ali J. Eisner.
Kazi	The Dragon Prince	Ashleica Edmond	2018–2024	After the release of the third season, the official Dragon Prince Twitter account revealed that Kazi, the Sunfire Elf sign language interpreter, goes by they/them pronouns.
Mr. Bon Kurei	One Piece	Kazuki Yao	1999–present	Also known as Bentham of the Wild and "Mr. 2 Bon Clay," he has powers that allows him to shapeshift into any form he wishes and crossdresses as a woman, and has a kempo called the "Okama Kempo". He is a recurring character, has defeated many villains, and was once an enemy of the Straw Hat Grand Fleet. He is part of "Baroque Works," and has been described as being "associated with queer gender identities."
Amber McKnight	Velma	Sara Ramirez	2023–2024	Amber is the child of Hex Girl Thorn. Amber uses they/them pronouns, and they are voiced by non-binary actor Sara Ramirez.
Marshmallow	The High Fructose Adventures of Annoying Orange	Dane Boedigheimer	2012–2014	Marshmallow is one of Annoying Orange's friends. On June 17, 2022, Marshmallow was confirmed to be non-binary. Marshmallow is voiced by creator Dane Boedigheimer who is transgender and non-binary.
Morph	X-Men: The Animated Series	Rob Rubin	1992–1997	In X-Men: The Animated Series, Morph, a mutant member of the X-Men with the power of shapeshifting, was presented as a male mutant who was referred to with masculine pronouns. Conversely, X-Men '97 presents Morph as non-binary and preferring they/them pronouns. Morph also shows romantic interest in Wolverine and confesses their love towards him while taking on the form of Jean Grey which was confirmed by showrunner Beau DeMayo. Morph is voiced by gay and non-binary voice actor J. P. Karliak. In the series itself, Morph is referred to with both they/them and he/him pronouns.
Morph	X-Men '97	J. P. Karliak	2024–present	In X-Men: The Animated Series, Morph, a mutant member of the X-Men with the power of shapeshifting, was presented as a male mutant who was referred to with masculine pronouns. Conversely, X-Men '97 presents Morph as non-binary and preferring they/them pronouns. Morph also shows romantic interest in Wolverine and confesses their love towards him while taking on the form of Jean Grey which was confirmed by showrunner Beau DeMayo. Morph is voiced by gay and non-binary voice actor J. P. Karliak. In the series itself, Morph is referred to with both they/them and he/him pronouns.
Nightshade	Transformers: EarthSpark	Z Infante	2022–present	One of the new members of Terrans, Transformers born on Earth and ally of the Autobots and the Malto family who first appears in the two-part episode "Age of Evolution". Nightshade explains to Optimus Prime that they go by they/them pronouns, as "he or she just doesn't fit who ".
Obsidian	Steven Universe	Various actors overlaid	2013–2019	A fusion of Steven Universe and fellow Crystal Gems Garnet, Amethyst and Pearl, Obsidian is based on the volcanic glass obsidian. Obsidian first appeared in the episode "Change Your Mind." They later were imagined in the episode "In Dreams," and shown in a flashback in "Growing Pains." Joe Johnson, a storyboard artist for the show confirmed that Obsidian uses they/them and she/her pronouns.
Okamas	One Piece		1999–present	More than one person, but a whole group of individuals, and are named after the tricky and controversial Japanese word "okama".
Pirate Polly	We Baby Bears	Em Hagen	2022–present	Polly is a parrot who is also a pirate captain. When Polly returns in the season 2 episode "Polly's New Crew", Polly has come out as non-binary and is now using they/them pronouns. Polly is voiced by series production coordinator Em Hagen, who is non-binary.
Puddle	Summer Camp Island	Ethan Maher	2018–2023	An alien who uses they/them pronouns. Their husband, Alien King, is the king of their planet.
Rainbow Quartz 2.0	Steven Universe	Alastair James	2013–2020	A fusion of Steven Universe and fellow Crystal Gem Pearl, Rainbow Quartz 2.0 is based on the metal-coated crystal rainbow quartz. He debuts in "Change Your Mind" and reappears in "A Very Special Episode." Rainbow Quartz uses both they/them and he/him pronouns, the only fusion to use these pronouns together, as confirmed by Colin Howard, a character designer, former writer and storyboard artist for Steven Universe and Steven Universe Future.
Rainbow Quartz 2.0	Steven Universe Future	Alastair James	2013–2020	A fusion of Steven Universe and fellow Crystal Gem Pearl, Rainbow Quartz 2.0 is based on the metal-coated crystal rainbow quartz. He debuts in "Change Your Mind" and reappears in "A Very Special Episode." Rainbow Quartz uses both they/them and he/him pronouns, the only fusion to use these pronouns together, as confirmed by Colin Howard, a character designer, former writer and storyboard artist for Steven Universe and Steven Universe Future.
River	Rubble & Crew	Chinag Ma	2023–present	River is a non-binary skateboarder. River uses they/them pronouns, and they are voiced by non-binary actor Cihang Ma.
Trixie Roughouse	Epithet Erased	Oz Ryan	2019	Trixie was confirmed to be non-binary and uses she/her, he/him and they/them pronouns.
Rusty	Thomas the Tank Engine & Friends	Matt Wilkinson	1984–2021	Rusty is an anthropomorphic narrow gauge diesel engine who works on the Skarloey Railway. Show developer Britt Allcroft had the intention of making Rusty a "gender-neutral" character, being neither male or female. Initially, beginning with the character's debut in series 4, dialogue and narration would avoid referring to Rusty with any gender specific pronouns. However, starting with the series 9 episode "Tuneful Toots", Rusty would instead be referred to with masculine pronouns.
Sailor Star Fighter	Sailor Moon	Shiho Niiyama	1992–1997	In the 90s anime, the Sailor Starlights were assigned female at birth, but transform to present as male and refer to themselves as males when not fighting, as shown in the episode, "Holy War in the Galaxy! Sailor Wars Legend." In a 1999 interview, Naoko Takeuchi, who wrote the Sailor Moon manga, said she had made them always girls in the manga, in the anime "they got turned into guys, and I wasn't very happy about it."
Sailor Star Healer	Sailor Moon	Chika Sakamoto	1992–1997	In the 90s anime, the Sailor Starlights were assigned female at birth, but transform to present as male and refer to themselves as males when not fighting, as shown in the episode, "Holy War in the Galaxy! Sailor Wars Legend." In a 1999 interview, Naoko Takeuchi, who wrote the Sailor Moon manga, said she had made them always girls in the manga, in the anime "they got turned into guys, and I wasn't very happy about it."
Sailor Star Maker	Sailor Moon	Narumi Tsunoda	1992–1997	In the 90s anime, the Sailor Starlights were assigned female at birth, but transform to present as male and refer to themselves as males when not fighting, as shown in the episode, "Holy War in the Galaxy! Sailor Wars Legend." In a 1999 interview, Naoko Takeuchi, who wrote the Sailor Moon manga, said she had made them always girls in the manga, in the anime "they got turned into guys, and I wasn't very happy about it."
Sailor Uranus	Sailor Moon Crystal	Junko Minagawa	2014–2016	Haruka is referred to by her partner, Sailor Neptune, as "both male and female, but also neither", making Haruka the first character in the franchise to be referred to that way. She is also said to have an independent attitude and not care much for teamwork. She also reappears in the film, Sailor Moon Eternal, as does Neptune.
Natsuru Senō	Kämpfer	Marina Inoue	2009	Natsuru is a second-year student at Seitetsu High School and has a crush on Kaede Sakura, one of the school's beauties. At the start of the story, he discovers that he has transformed into a girl, and learns that he has been chosen to be a Kämpfer with Zauber, or magic, powers such as casting fireballs from the beginning of the series. As a girl, he has longer hair styled in a ponytail. After a fight with Shizuku causes him to expose his Kämpfer form to other students of the school, Natsuru is enrolled as a girl of the same name at the school. Natsuru's female form becomes the subject of intense affection from Kaede Sakura, nearly the entire female student body, and the boys, including his male classmates.
Natsuru Senō	Kämpfer	Sabrina Owen	2011	Natsuru is a second-year student at Seitetsu High School and has a crush on Kaede Sakura, one of the school's beauties. At the start of the story, he discovers that he has transformed into a girl, and learns that he has been chosen to be a Kämpfer with Zauber, or magic, powers such as casting fireballs from the beginning of the series. As a girl, he has longer hair styled in a ponytail. After a fight with Shizuku causes him to expose his Kämpfer form to other students of the school, Natsuru is enrolled as a girl of the same name at the school. Natsuru's female form becomes the subject of intense affection from Kaede Sakura, nearly the entire female student body, and the boys, including his male classmates.
Shep	Steven Universe Future	Indya Moore	2020	Shep is the partner of Sadie Miller, voiced by Indya Moore who is also non-binary, transgender, uses gender neutral they/them pronouns, and is a person of color. In their episode debut in "Little Graduation", Shep helped Steven work out his mental problems and come to his senses.
Smoky Quartz	Steven Universe	Natasha Lyonne	2013–2020	A fusion of Steven Universe, and fellow Crystal Gem, Amethyst, Smoky is based on the crystal smoky quartz. Steven and Amethyst first fused at a low point for both characters as noted by Michaela Dietz, the voice actress for Amethyst on the official Steven Universe podcast. Smoky debuted in the episode "Earthlings," and reappeared in three other episodes: "Know Your Fusion," "Change Your Mind", and "Guidance." Rebecca Sugar has stated that the Gems are "all non-binary women," with this applying to Amethyst specifically. On November 10, 2017, Cartoon Network posted a YouTube video of the character Peridot playing Steven Universe: Save the Light, in which she refers to Smoky with the singular they pronoun.
Smoky Quartz	Steven Universe Future	Natasha Lyonne	2013–2020	A fusion of Steven Universe, and fellow Crystal Gem, Amethyst, Smoky is based on the crystal smoky quartz. Steven and Amethyst first fused at a low point for both characters as noted by Michaela Dietz, the voice actress for Amethyst on the official Steven Universe podcast. Smoky debuted in the episode "Earthlings," and reappeared in three other episodes: "Know Your Fusion," "Change Your Mind", and "Guidance." Rebecca Sugar has stated that the Gems are "all non-binary women," with this applying to Amethyst specifically. On November 10, 2017, Cartoon Network posted a YouTube video of the character Peridot playing Steven Universe: Save the Light, in which she refers to Smoky with the singular they pronoun.
Frankie Stein	Monster High	Iris Menas	2022	Frankie Stein is a monster who was created by scientists. Unlike the previous incarnations where Frankie was a cisgender woman and Frankenstein's monster and his bride's daughter, Frankie is a non-binary monster. In the series, and live-action movie, Frankie is non-binary and uses they/them pronouns. In the episode "Horoscare", they are revealed to have feelings for Cleo de Nile and Cleo is receptive towards them. Iris Menas, who voices Stein, is a non-binary actor.
Stevonnie	Steven Universe	AJ Michalka	2013–2020	Stevonnie is a fusion of both Steven and Connie. Steven and Connie identify as male and female respectively, but the gender of Stevonnie is difficult to describe, with series creator Rebecca Sugar describing it as the "living relationship between Steven and Connie." Stevonnie is commonly referred to with gender neutral pronouns, while male and female characters seem to be physically attracted to Stevonnie.
Stevonnie	Steven Universe Future	AJ Michalka	2013–2020	Stevonnie is a fusion of both Steven and Connie. Steven and Connie identify as male and female respectively, but the gender of Stevonnie is difficult to describe, with series creator Rebecca Sugar describing it as the "living relationship between Steven and Connie." Stevonnie is commonly referred to with gender neutral pronouns, while male and female characters seem to be physically attracted to Stevonnie.
Nathan Seymour	Tiger & Bunny	Kenjiro Tsuda	2011	Nathan is a highly effeminate homosexual man who identifies as genderqueer though he prefers to be identified as a woman at times, often spending more time with the female heroes while flirting with the male heroes. In the past, they tried to present themselves femininely but was harshly criticized, and they still hold a strong romantic infatuation towards men.
Nathan Seymour	Tiger & Bunny	John Eric Bentley	2011	Nathan is a highly effeminate homosexual man who identifies as genderqueer though he prefers to be identified as a woman at times, often spending more time with the female heroes while flirting with the male heroes. In the past, they tried to present themselves femininely but was harshly criticized, and they still hold a strong romantic infatuation towards men.
Sunstone	Steven Universe	Shoniqua Shandai	2013–2020	A fusion of Steven Universe and fellow Crystal Gem Garnet, Sunstone is based on the mineral sunstone. Debuting in "Change Your Mind" and reappearing in "A Very Special Episode", Sunstone uses both singular they and feminine pronouns as confirmed on the official Steven Universe podcast, with Sunstone's pronouns also confirmed in this episode.
Sunstone	Steven Universe Future	Shoniqua Shandai	2013–2020	A fusion of Steven Universe and fellow Crystal Gem Garnet, Sunstone is based on the mineral sunstone. Debuting in "Change Your Mind" and reappearing in "A Very Special Episode", Sunstone uses both singular they and feminine pronouns as confirmed on the official Steven Universe podcast, with Sunstone's pronouns also confirmed in this episode.
Tai	Moon Girl and Devil Dinosaur	Ian Alexander	2023–2025	Tai is one of Lunella Lafayette's classmates. Tai is revealed to be non-binary in the episode "Check Yourself" as Tai is referred to with they/them pronouns by Lunella and in the unaired episode "The Gatekeeper" Tai explicitly said they're non-binary. Tai is voiced by non-binary actor Ian Alexander.
Yoshino Takatsuki	Wandering Son	Asami Seto	2011	A student in Shuichi's class, also known as Takatsuki-kun by their classmates, is often seen as a girl, but wants to be a boy, often refraining from dressing in traditionally feminine clothes. He is attracted toward Shuichi and Saori, while he also experiences signs of gender dysphoria.
Tefé Holland	Harley Quinn	Vico Ortiz	2019–present	In this series, Tefé Holland is non-binary and uses they/them pronouns. Tefé Holland is voiced by non-binary actor Vico Ortiz.
Terri	Amphibia	Kate Micucci	2019–2022	Terri is a scientist who helped Anne Boonchuy and the Plantars. In the show, they are feminine-presenting and was referred to as a woman by Anne. In the 2022 book Marcy's Journal: A Guide to Amphibia, Marcy uses gender-neutral pronouns for them.
Thomas	City of Ghosts	Blue Chapman	2021	Thomas is a 7-year-old child who goes by they/them pronouns. They are voiced by transgender child actor Blue Chapman.
Raine Whispers	The Owl House	Avi Roque	2021–2023	Raine is the head witch of the Bard Coven and uses they/them pronouns. They are Disney TVA's first non-binary character. The episode "Knock, Knock, Knockin' on Hooty's Door" reveals that Eda Clawthorne and Raine were formerly dating, before breaking up.
Raine Whispers	The Owl House	Blu del Barrio	2021–2023	Raine is the head witch of the Bard Coven and uses they/them pronouns. They are Disney TVA's first non-binary character. The episode "Knock, Knock, Knockin' on Hooty's Door" reveals that Eda Clawthorne and Raine were formerly dating, before breaking up.
Kiui Watase	Jellyfish Can't Swim in the Night	Miyu Tomita	2024	In the 11th episode, she publicly expresses her gender fluidity, criticizing those who want her to behave in a "normal" manner, unashamed for embracing what she likes, and is supported by Yoru. She also is Mahiru's childhood friend and lends her talents to JELEE as its video editor and mixer.
Kiui Watase	Jellyfish Can't Swim in the Night	Molly Searcy	2024	In the 11th episode, she publicly expresses her gender fluidity, criticizing those who want her to behave in a "normal" manner, unashamed for embracing what she likes, and is supported by Yoru. She also is Mahiru's childhood friend and lends her talents to JELEE as its video editor and mixer.
Wren	Middle School Moguls	Tim Gunn	2019	One of the professors in the show, Mogul Wren, has been stated to be non-binary. They have a big role in the episode "Mo'gul Money, Mo Problems".
Wren	Pinecone & Pony	Ser Anzoategui	2022–2023	Wren is a non-binary person who prefers they/them pronouns, and is in a relationship with Gladys, the teacher for the Lil Rumblers. Wren is voiced by non-binary actor Ser Anzoategui.
Shion Zaiden	RWBY: Ice Queendom	Hiroki Nanami	2022	A nightmare hunter who captures Grimm who possess people in their dreams. Their presence in the series was praised by some reviewers. While the official Japanese website, refers to Shion with male pronouns, Rooster Teeth treats Shion as nonbinary. Christine Brent, Senior Brand Director for Rooster Teeth, described Shion as fantastic, "totally nonbinary", and unique to this series, while using they/them pronouns for Shion, and said she would like to have similar characters in future productions.