| Characters | Title | Character debut date | Notes | Country |
| Ryo Asuka | Devilman Crybaby | | Ryo Asuka is a genderless angel who is Akira Fudo's best friend, while Akira is the protagonist. Akira was the first person to show him kindness, causing him to become deeply attached. Near the end, Ryo remembers he is Satan, an intersex fallen angel who fell out of favor with God when he challenged God's authority for killing the demons and replacing them with humans. Satan set out to start a war between humans and demons and was reborn as a male. He fell in love with Akira and subconsciously had him turned into a devilman to ensure he would survive the war and be by his side. By the end of the series, Satan realizes he loves Akira despite having previously believed love did not exist and cries and mourns Akira's death. Akira is in a relationship with a woman, but his feelings towards Ryo have been described as homoerotic. | Japan |
| Crimvael "Crim" | Interspecies Reviewers | | A well-endowed intersex angel with a broken halo, Crimvael has male and female genitalia, as noted throughout the series. Despite Crim’s feminine appearance, they chooses to identify as male upon meeting Stunk and Zel in episode 1, to avoid the two from trying anything perverted on them. Crim hinted to be bisexual in episode 3 and in episode 9, the narrator uses the pronoun "they" to refer to Crim. This is due to Crim having no set gender, much like the rest of the angels, and the narrator takes note on that. | Japan |
| Luca Esposito | Astra Lost in Space | | Luca, in the anime and manga Astra Lost in Space, is an artist and talented engineer on board the Astra. He was raised as and mostly identifies as a boy, and comes out as intersex halfway through the story, in the episode "Secret", not considering himself a man or a woman. His characteristics imply he most likely has Klinefelter syndrome or a mild form of androgen insensitivity syndrome, and was described by Michele Liu of Anime News Network as "unique" since Luca is a main character which is born intersex rather than "altered by sci-fi space diseases or external influence," with Liu also describing Luca as bisexual. | Japan |
| Iena Madaraba | Seton Academy: Join the Pack! | | Iena Madaraba, also known as Yena or Hyena, is a spotted hyena with female genitalia and is confused about her true gender & sexuality. Even after her real gender is proven, Hitomi reassures Iena to just be the tomboyish she wants. It is hinted on multiple occasions that Iena and Hitomi have mutual feelings for each other. In the future, Iena is a martial artist. After failing to feel feminine in various episodes, she claims out that she will still be a male inside in episodes such as "The Wild Habits of a Troubled Animal". Crunchyroll described Iena as a "gender-ambiguous hyena person." | Japan |
| Ruby Moon | Cardcaptor Sakura | | Ruby Moon has no biological sex. They are always seen presenting as female, even though Spinel Sun often asks her why don't they present as male for a change as shown in episodes like "Sakura and the Mysterious Transfer Student". At some points, Ruby Moon states that their gender does not matter because they are not actually human and also that girls have cuter clothes. They appear to have a crush on Touya. Like Syaoran, Sakura, Touya, Yukita, and Tomoyo, they also reappear in Cardcaptor Sakura: Clear Card. | Japan |
| Stevonnie | Steven Universe | | Stevonnie is a fusion of both Steven and Connie. Steven and Connie identify as male and female respectively, but the gender of Stevonnie is difficult to describe, with series creator Rebecca Sugar describing it as the "living relationship between Steven and Connie." Stevonnie is commonly referred to with gender neutral pronouns, while male and female characters seem to be physically attracted to Stevonnie. Stevonnie also appears in a non-speaking role in the episode "Bismuth Casual" of the limited epilogue series, Steven Universe Future. In an in-universe public service announcement about self esteem, Stevonnie is specifically labeled as intersex. | United States |
| Stevonnie | Steven Universe Future | | Stevonnie is a fusion of both Steven and Connie. Steven and Connie identify as male and female respectively, but the gender of Stevonnie is difficult to describe, with series creator Rebecca Sugar describing it as the "living relationship between Steven and Connie." Stevonnie is commonly referred to with gender neutral pronouns, while male and female characters seem to be physically attracted to Stevonnie. Stevonnie also appears in a non-speaking role in the episode "Bismuth Casual" of the limited epilogue series, Steven Universe Future. In an in-universe public service announcement about self esteem, Stevonnie is specifically labeled as intersex. | United States |
| Izana Shinatose | Knights of Sidonia | | Izana belongs to a new, nonbinary third gender that originated during the hundreds of years of human emigration into space, as first shown in the episode "Commencement." Izana later turns into a girl after falling in love with Nagate Tanasake. | Japan |
| Characters | Name of comic | Years | Notes |
| Aggie | Go Get a Roomie! | 2010–Present | In this comic, which explores sexual themes, Aggie, Roomie's past lover, reveals she was born intersex in a radio interview. |
| Belzeba | Belzeba figlia del Diavolo | 1977-1978 | In this erotic Italian comic, Belzeba is the intersex daughter of the Devil. |
| Comet | Adventure Comics | 1989-1991 | Comet is an intersex character, who changed their form from female to male, and Comet later began relationship with Blithe, since she loved both their forms. |
| Comet | Action Comics | 1989-1991 | Comet is an intersex character, who changed their form from female to male, and Comet later began relationship with Blithe, since she loved both their forms. |
| Comet | Supergirl | 1989-1991 | Comet is an intersex character, who changed their form from female to male, and Comet later began relationship with Blithe, since she loved both their forms. |
| Desire | The Sandman | 1989–Present | Desire is one of the Endless. |
| Rebis | Doom Patrol | 1963-1968 | Rebis is a character in the comic series Doom Patrol. Formed from the fusion of Negative Man, the negative spirit inhabiting him, and his doctor Eleanor Poole, Rebis identifies as both male and female. |
| Richard Plantagenet, later Richard III of England | Requiem of the Rose King | 2013–Present | In this manga adaptation of Richard III, Richard is portrayed as intersex instead of hunchbacked as the character is normally depicted in the original play. |
| Ystina, the Shining Knight | Demon Knights | 2011-2013 | Ystina is revealed as DC Comics's first intersex character in Demon Knights #14. |
| Characters | Work | Author | Years | Description |
| Cornelius Brunner | The Final Programme | Michael Moorcock | 1965 | Cornelius Brunner, the result of a physical merging of the protagonist Jerry Cornelius and Miss Brunner in this novel. |
| Therem Harth rem ir Estraven | The Left Hand of Darkness | Ursula K. Le Guin | 1969 | Therem is a character in this science fiction novel about a planet whose inhabitants have no genitalia or secondary sex characteristics, except for one week every month where they get the urge to mate, and temporarily develop either male or female genitalia and gametes.
After the reproduction is finished, the "male" returns to their neutral state and the "female" carries the baby and then returns to their neutral state. Thus, the planet has no concept of gender distinction, and, since anyone could get pregnant, childcare and family planning are easily accessed. |
| Fawn Singleton Farrell | No Matter How Much You Promise to Cook or Pay the Rent You Blew It Cauze Bill Bailey Ain't Never Coming Home Again | Edgardo Vega Yunqué | 2003 | Fawn Singleton Farrell is the half-sister of main character Vidamía Farrell, in this novel. |
| Swan Er Hong | 2312 | Kim Stanley Robinson | 2012 | Swan Er Hong is the lead character in this science fiction novel. |
| Ilario | Ilario, A Story of the First History | Mary Gentle | 2006-2007 | Ilario is the lead character in this set of alternate history novels. |
| Keral | The World Wreckers | Marion Zimmer Bradley | 1971 | Keral is a member of an alien species in this science fiction novel. |
| Kirsten Lattimer | None of the Above | I. W. Gregorio | 2015 | Kirsten, homecoming queen and champion hurdler, finds her life turned upside-down after a diagnosis with androgen insensitivity syndrome in this novel. |
| Callie Stephanides | Middlesex | Jeffrey Eugenides | 2002 | Callie "Cal" Stephanides is an intersex character in this novel. |
| Bel Thorne | Vorkosigan Saga | Lois McMaster Bujold | 1986–Present | Bel Thorne is an intersex ship captain in this series of science fiction novels and uses it/its pronouns. |
| Fitz Wahram | 2312 | Kim Stanley Robinson | 2013 | Fitz Wahram is an intersex character in this science fiction novel. |
| Max Walker | Golden Boy | Abigail Tarttelin | 2013 | Max is a lead character in this award-winning novel. |
| Annabel/Wayne | Annabel | Kathleen Winter | 2010 | Annabel/Wayne is an intersex character in this novel who was born intersex but is raised as a man. |
| Wraeththu | Wraeththu | Storm Constantine | 1987-1993 | Wraeththu are a post-apocalyptic species in this set of novels. |
| Sadako Yamamura | Ring | Koji Suzuki | 1991-2013 | Sadako Yamamura is an antagonist in this novel series, later made into a film. |
| Characters | Actor | Show | Years | Notes |
| Amy Andrews | Jessica Campbell | Freaks and Geeks | 1999-2000 | Amy, girlfriend of Ken Miller in teen comedy/drama TV series, was said to be a groundbreaking intersex character on television. |
| Buba | Maria Luisa Mendonça | Renascer | 1993 | Buba had a romantic relationship with João Pedro, one of the sons of José Inocêncio, the protagonist of the soap opera. At the time, the term "hermaphrodite" was used to describe Buba's variation. |
| Cacau | Not known | Renascer | 2024 | Teca, Cacau's mother, gives birth with the help of Morena, Buba and Inácia. Cacau's intersexness is revealed by the doctor. |
| Vincent Clarkson | Daphnée Duplaix, Phillip Jeanmarie, Siena Goines | Passions | 1999-2008 | An intersex character in this NBC/DirecTV soap opera who becomes pregnant with his father's son. |
| Lauren Cooper | Bailey De Young | Faking It | 2014-2018 | Lauren is a character in MTV scripted comedy and said to be the first intersex series regular on television. |
| Lorraine Finster | Minnie Driver | Will & Grace | 1998–2006
2017-2020 | In the season 6 episode "The Accidental Tsuris", recurring character Lorraine is established to have been born intersex. Lorraine's parents had her operated on, after which they raised Lorraine as a girl. |
| Jake | Not known | Emily Owens, M.D. | 2012-2013 | An intersex baby in episode "Emily and... the Question of Faith". |
| Peta | Leeanna Walsman | Janet King | 2014-2017 | Peta is character revealed as intersex in the final episode of the second Janet King series. |
| Raven | Amanda Saenz | Faking It | 2014-2018 | Raven was notable for being the first intersex character played by an openly intersex person. |
| Alexandria 'Alex' Robinson | Cameron Richardson | House | 2004-2012 | Alex, a patient in the season 2 episode "Skin Deep", is revealed to be intersex. The handling of the character was labeled offensive by the Intersex Society of North America |
| Toby | Beattie Edmondson | Upstart Crow | 2016–present | The series 3 episode "The Most Unkindest Cut of All!" features Toby, an Elizabethan actor who is intersex. |