| No. | Portrait | Name | President | Birth | Death | Period |
| 1 | | María de la Ossa de Amador | Manuel Amador Guerrero | March 1, 1855 | July 5, 1948 | February 20, 1904 – October 1, 1908 |
| | Manuela Jované Aguilar y Távara | José Domingo de Obaldía | | 1887 | |
| | Josefa Jované Aguilar y Távara | José Domingo de Obaldía | | | October 1, 1908 – March 1, 1910 |
| | Rita Barsallo de Mendoza | Carlos Antonio Mendoza | | | March 1, 1910 – October 1, 1910 |
| | Teodolinda Briceño de Boyd | Federico Boyd | | | October 1, 1910 - October 5, 1910 |
| | Matilde Esperanza Picón Herrera | Pablo Arosemena | | | October 5, 1910 – October 1, 1912 |
| | Eva Paniza Arosemena | Belisario Porras | | | |
| | Alicia Castro Gutiérrez de Porras | Belisario Porras | | | October 1, 1912 - October 1, 1916 |
| | Diana Dutary Pérez | Ramón Maximiliano Valdés | | | October 1, 1916 – June 3, 1918 |
| | Kerima Gutiérrez de Díaz | Pedro Antonio Díaz | | | October 1, 1918 - October 12, 1918 |
| | Alicia Castro Gutiérrez de Porras | Belisario Porras | | | October 12, 1918 - January 30, 1920 |
| | Oderay Arango de Lefevre | Ernesto T. Lefevre | | | January 30, 1920 - October 1, 1920 |
| | Ofelina Remón de Chiari | Rodolfo Chiari | | | October 1, 1924 – October 1, 1928 |
| | Hersilia Arias de Arosemena | Florencio Harmodio Arosemena | | | October 1, 1928 - January 3, 1931 |
| | Rosario Guardia de Arias | Harmodio Arias Madrid | | | January 3, 1931 - January 16, 1931 |
| | Amelia Lyons de Alfaro | Ricardo J. Alfaro | December 15, 1883 | November 27, 1973 | January 16, 1931 - June 5, 1932 |
| | Rosario Guardia de Arias | Harmodio Arias Madrid | | | June 5, 1932 - October 1, 1936 |
| | Malvina Galindo de Arosemena | Juan Demóstenes Arosemena | | | October 1, 1936 - December 16, 1939 |
| | Adela Moré de Fernández | Ezequiel Fernández Jaén | | | December 16, 1939 - December 18, 1940 |
| | Raquel de la Guardia de Boyd | Augusto Samuel Boyd | | | December 18, 1940 - October 1, 1940 |
| | Ana Matilde Linares de Arias | Arnulfo Arias Madrid | | | October 1, 1940 - October 9, 1941 |
| | Vacant | Ricardo de la Guardia | | | October 9, 1941 - March 4, 1943 |
| | Carmen Estripeaut de la Guardia | Ricardo de la Guardia | | | March 4, 1943 - June 15, 1945 |
| | Beatriz de la Guardia de Jiménez | Enrique A. Jiménez | | | June 15, 1945 - August 7, 1948 |
| | Celia Quelquejeu de Díaz | Domingo Díaz Arosemena | | | August 7, 1948 - July 28, 1949 |
| | Isabel Icaza de Chanis | Daniel Chanis Pinzón | | | July 28, 1949 - November 25, 1949 |
| | Cecilia Orillac de Chiari | Roberto F. Chiari | | | November 20, 1949 - November 24, 1949 |
| | Ana Matilde Linares de Arias | Arnulfo Arias Madrid | | | November 24, 1949 - May 9, 1951 |
| | Heliodora Arosemena de Arosemena | Alcibíades Arosemena | | | May 9, 1951 - October 1, 1952 |
| | Cecilia Pinel de Remón | José Remón Cantera | | | October 1, 1952 - January 2, 1955 |
| | Olga Arias de Arias | Ricardo Arias Espinosa | | | March 29, 1955 - October 1, 1956 |
| | Mercedes Galindo de la Guardia | Ernesto de la Guardia | | | October 1, 1956 - October 1, 1960 |
| | Cecilia Orillac de Chiari | Roberto F. Chiari | | | October 1, 1960 - October 1, 1964 |
| | Petita Saa de Robles | Marco Aurelio Robles | | | October 1, 1964 - October 1, 1968 |
| | Mireya Moscoso de Arias | Arnulfo Arias Madrid | July 1, 1946 | | October 1, 1968 - October 11, 1968 |
| | Elizabeth "Fanny" Rojer de Lakas | Demetrio B. Lakas | | | December 19, 1969 – October 11, 1978 |
| | Adela Ruiz de Royo | Arístides Royo | December 15, 1943 | June 19, 2019 | October 11, 1978 – July 31, 1982 |
| | Mercedes Martinez de la Espriella | Ricardo de la Espriella | | | July 31, 1982 – February 13, 1984 |
| | Luzmila Arosemena de Illueca | Jorge Illueca | | | February 13, 1984 - October 11, 1984 |
| | María Consuelo Rivera de Ardito Barletta | Nicolás Ardito Barletta | | | October 11, 1984 - September 27, 1985 |
| | Mariela de Delvalle | Eric Arturo Delvalle | | | September 28, 1985 - February 26, 1988 |
| | Marcela Endara Cambra | Guillermo Endara | | June, 1989 | December 20, 1989 - June 11, 1990 |
| | Ana Mae Diaz | Guillermo Endara | May 16, 1967 | | June 11, 1990 – September 1, 1994 |
| | Dora Boyd de Perez Balladares | Ernesto Pérez Balladares | October 11, 1948 | | September 1, 1994 – September 1, 1999 |
| | Ruby Moscoso | Mireya Moscoso | September 22, 1941 | January 7, 2022 | September 1, 1999 – September 1, 2004 |
| | Vivian Fernández | Martín Torrijos | March 25, 1966 | | September 1, 2004 – July 1, 2009 |
| | Marta Linares | Ricardo Martinelli | September 2, 1956 | | July 1, 2009 - July 1, 2014 |
| | Lorena Castillo | Juan Carlos Varela | July 31, 1968 | | July 1, 2014 - July 1, 2019 |
| | Yazmín Colón de Cortizo | Laurentino Cortizo | July 31, 1959 | | July 1, 2019 - July 1, 2024 |
| | Maricel Cohen de Mulino | José Raúl Mulino | | | July 1, 2024 - Present |