| Villain | First appearance | Description |
| General Immortus | My Greatest Adventure #80 | Immortal “general” of a cultish criminal syndicate with centuries of knowledge about military tactics. |
| The Brain | Doom Patrol#86 | Disembodied brain of scientific genius, the Brain led the Brotherhood of Evil. |
| Madame Rouge | Doom Patrol#86 | Instructor at a French girls’ school who was secretly the deadly Madame Rouge, with an elastic body and the ability to remold her face to imitate anyone. |
| Monsieur Mallah | Doom Patrol#86 | A gorilla who was chosen by the Brain to be subjected to training and electric shocks that turned him into a genius. |
| Mr. Nobody | Doom Patrol #86 ; #26 | Mr. Morden, a “one-man crime wave,” stole giant robot Rog in his quest to join the Brotherhood of Evil; driven insane and turned into an abstract shadow form by an ex-Nazi scientist, he gained the power to drain sanity from his victims and rechristened himself Mr. Nobody, leader of the Brotherhood of Dada. |
| Animal-Vegetable-Mineral Man | Doom Patrol #89 | Dr. Sven Larsen, Swedish scientist and former student of the Chief, fell into a vat simulating the organic soup of the primordial Earth and gained power to change his body into any form of animal, vegetable, or mineral. |
| Garguax | Doom Patrol #91 | Mammoth, obese forerunner of an alien invasion force who used the advanced technology of his world, including an android army dubbed the Plastic Men. |
| Mr. 103 | Doom Patrol #98 | John Dubrovny was a genius biochemist who suffered a mental collapse and developed the power to transform himself into any element on the atomic table. |
| General Zahl | Doom Patrol #121 | Originally called Captain Zahl, the former Nazi U-boat captain was a frequent sparring partner with the Doom Patrol. His most notable claim is his role as the mastermind in the believed demise of the group alongside Madame Rouge. |
| Red Jack | Doom Patrol #23 "The Butterfly Collector" | Arguably the most powerful villain the team has ever encountered, Red Jack was an extra-dimensional being, with a filigree crown and a masquerade-style mask, dressed in mid 17th century nobleman costume, who claimed to be both Jack the Ripper, and the creator of the universe. His powers derived from the collective suffering of millions of butterflies, which he kept pinned to the wall of his home. He kidnapped Rhea Jones from the hospital where she was being tended to during a coma, in order to make her his bride. Upon discovering Rhea missing from her hospital bed, Crazy Jane reveals the identity of her kidnapper through a divining ritual involving cut up pieces from books she stole from a store down the street. Red Jack easily defeats the Doom Patrol, and seems unstoppable until Crazy Jane releases his butterfly collection, rendering him powerless, and allowing a suddenly-awakened Rhea Jones to stab him in the back with his own knife. |
| Shrapnel | Doom Patrol vol. 2 #7 | Mark Scheffer was a serial bomber and government anarchist before a lab accident turned his skin into an organic metal compound giving him superhuman strength, speed, and the ability to manipulate metal projectiles from his body into explosive bursts. He was discovered by the Doom Patrol in Kansas while on a murder spree and forced into combat by the team. |
| Villain | First appearance | Description |
| Doctor Janus | My Greatest Adventure #81 | Josef Kreutz, formerly a propagandist for Hitler, escaped the fall of Nazi Germany and invented a radio-like device that made people see things that were not there. |
| The Green-Headed League | My Greatest Adventure #82 | Three green-skinned aliens disguised themselves as human and infiltrated the corridors of power over seven years of planning as Senator Durham, political powerhouse; Dr. Savatini, chairman of the International Science Foundation; and Monsieur Duvoir, famed financier. |
| Cooky and Nolan | My Greatest Adventure #83 | Basic crooks exploiting power outages caused when Negative Man’s energy form was unleashed out of control. |
| The Nuclear Beasts | My Greatest Adventure #85 | Two nuclear-powered creatures from the Earth’s core. |
| Giacamo | Doom Patrol vol 1 #87 | A little person who served the Brotherhood of Evil. |
| Vince Harding | Doom Patrol vol 1 #87 | Escaped killer who hid on a Pacific island loaded with deadly booby-traps. |
| The Baron | Doom Patrol vol 1 #88 | Monocle- and cape-wearing gentlemanly leader of thieves using advanced technology to execute stylish and daring plans, all in order to seize wealth to be used by General Immortus. |
| S/Sgt. Allen Norton | Doom Patrol vol 1 #89 | Amnesiac war veteran with post-traumatic stress disorder that turned him violent when he heard sounds reminiscent of machine gun fire. |
| Dr. Tyme | Doom Patrol vol 1 #92 | Invented the “4-X beam” that slows or accelerates time in localized areas, and which he radiated from the top of his absurd alarm-clock shaped helmet. Hid in “Merlin’s Castle,” which he decorated with a large number of clocks. |
| Osra | Doom Patrol vol 1 #94 | Dr. John Radick, author of best-seller “Illusion and Reality,” was secretly the son of Dr. Janus and used Janus’s mental illusion radio to create the belief that Osra, the ghost of an ancient sultan, was destroying buildings. |
| The Claw | Doom Patrol vol 1 #94 | Hawk-masked thief who controlled super falcons Meena and Tonka, which had steel razor tipped claws and could fly at speeds topping 200 miles per hour. |
| The Bug Man | Doom Patrol vol 1 #99 | Used army of robots and a flying robot vehicle that could transform into simulacra of bugs. |
| King of Dinosaurs | Doom Patrol vol 1 #100 | Dr. Weir went mad and hypnotized a young green-skinned boy named Craig in order to steal his key to a bank deposit box which held Craig’s father’s secrets of anti-evolution, which he used to engineer dinosaurs he controlled in a series of mad thefts. |
| Nicholas Galtry | Doom Patrol vol 1 #100 | Guardian of the young man who was secretly Beast Boy, he stole from the boy’s trust and hired an assassin to kill him to prevent the embezzlement from being discovered. |
| League of Challenger Haters | Challengers of the Unknown #42 | A villain group that usually antagonizes the Challengers of the Unknown. It consists of Multi-Man, Kra, Multi-Woman, and Volcano Man. The Doom Patrol once fought the League of Challenge Haters in "Doom Patrol" #102 when they tried to find Atlantis causing the Doom Patrol and the Challengers of the Unknown to team up to stop them. |
| King Zatopa | Doom Patrol vol 1 #102 | Leader of an Atlantean nation that was preserved in suspended animation and revived by Multi-Man and the Challenger-Haters, who held the nation’s queen hostage to force Zatopa and his army to work on their behalf. |
| The Meteor Man | Doom Patrol vol 1 #103 | Professor Randolph Ormsby, germ-phobic astronomer, was transformed by cosmic rays into a rampaging living meteor growing ever more powerful as he absorbed iron into his molten core. |
| Ultimax | Doom Patrol vol 1 #107 | Rampaging giant robot intending to create a world of living computers, who was armed with techniques to neutralize all of the Doom Patrol, including a gas that super-activated Elasti-Girl’s shrinking powers and sent her into a subatomic universe. Ultimately revealed to be a pawn of the Brotherhood of Evil. |
| Doctor Death | Doom Patrol vol 1 #107 | A disfigured, skull-faced man named Dr. Drew invented an energy shroud which he intended to use to strangle the planet and destroy all life, until Negative Man thwarted his scheme. He was obsessed with making Negative Man realize he was so freakish that he could work only with a fellow freak like himself. |
| Abu Hallam | Doom Patrol vol 1 #107 | Abu Hallam, masked like a witch doctor, infiltrated and took over Swiss bank C.G.Y., thwarting Steve Dayton’s attempt to uncover Nicholas Gantry’s financial crimes, until confronted by Steve Dayton and he revealed himself to be Monsieur Mallah in disguise. |
| The Zarakas | Doom Patrol vol 1 #107 | One of two warring nations in a subatomic world, led by Toxino, the one-eyed king, who was nearly deposed by Count Waja. |
| Mandred the Executioner | Doom Patrol vol 1 #109 | A single, powerful entity created by the sacrifice of Garguax’s Plastic Men androids, which threw themselves into a vat to pool themselves into one creature. |
| Zarox-13 | Doom Patrol vol 1 #111 | Thirteenth leader of Garguax’s alien race, who was revered by the epithets “Master of the Dark Forces, Ruler of the Ruthless, Protector of the Unholy, and Emperor of the Cosmos.” It took the combined forces of the Doom Patrol and the Brotherhood of Evil to stop Zarox-13. |
| The Arsenal | Doom Patrol vol 1 #113 | A large mecha-suit, powered with an arsenal of weapons intended to neutralize the Doom Patrol’s powers, operated by a dwarf. |
| Arsenal | Doom Patrol vol 1 #113 | An anti-social, criminal inventor who combated his restricted height by transferring his mind into a robot body equipped with various high powered firearms and melee weapons. He first came into the criminal landscape as a mercenary for hire. |
| Kor, the Conqueror | Doom Patrol vol 1 #114 | Prof. Anton Koravyk, specialist in sonic technology, attempted to send himself back in time to escape the governments intent on channeling his talents into military uses, but accidentally punched through time and de-evolved himself into a Neanderthal warrior. |
| The Mutant Trio | Doom Patrol vol 1 #115 | Three telepathically linked flying mutants with three-fingered hands and destructive power claimed to be aliens but were deformed by atom bomb radiation, “the first children of the atomic age.” |
| The Black Vulture | Doom Patrol vol 1 #117 | A man named Decker attempted to swindle a Native American tribe out of its land, calling himself “Son of Geronimo,” until the Chief discovered his plot. Decker then sought revenge as the Black Vulture, a bird-costumed man flying by jet-powered gauntlets, wielding steel-claw talons, and controlling a menageries of superbirds. |
| Videx | Doom Patrol vol 1 #118 | Jalmar Lichtmeister believed he had uncovered the secret to invisibility and tested his theories on himself, successfully turning his outer skin invisible but revealing his viscera and gaining power over light. |
| The Great Guru | Doom Patrol vol 1 #119 | Yaramishi Rama Yogi used radical “therapy” to attempt to emotionally cripple the Doom Patrol in order to distract them while he “liberated” Madame Rouge from her brief romance with the Chief. |
| The Wrecker | Doom Patrol vol 1 #120 | Harvey Keller, twin brother of artist Morton Keller, was distraught when Morton was killed by a medical error when a hospital data processor mislabelled his blood type. Keller created New World Island, a Sargasso sea in space of satellites and space craft, from which he plotted to use technology destructively in order to wean mankind from his dependence on technology. |
| Scissormen | Doom Patrol vol 2 #19 | The scissormen are from the metafictional city of Orqwith. |
| The Beard Hunter | Doom Patrol vol 2 #45 | Ernest Franklin was a disturbed and closeted gay assassin of bearded men who was hired the Bearded Gentlemen's Club of Metropolis to kill the Chief because he wouldn't sell his beard to them. He cannot grow a beard due to a male hormone deficiency according to his mother when she was visited by the police. |
