Viral vector


A viral vector is a modified virus designed to deliver genetic material into cells. This process can be performed inside an organism or in cell culture. Viral vectors have widespread applications in basic research, agriculture, and medicine.
Viruses have evolved specialized molecular mechanisms to transport their genomes into infected hosts, a process termed transduction. This capability has been exploited for use as viral vectors, which may integrate their genetic cargo—the transgene—into the host genome, although non-integrative vectors are also commonly used. In addition to agriculture and laboratory research, viral vectors are widely applied in gene therapy: as of 2022, all approved gene therapies were viral vector-based. Further, compared to traditional vaccines, the intracellular antigen expression enabled by viral vector vaccines offers more robust immune activation.
Many types of viruses have been developed into viral vector platforms, ranging from retroviruses to cytomegaloviruses. Different viral vector classes vary widely in strengths and limitations, suiting some to specific applications. For instance, relatively non-immunogenic and integrative vectors like lentiviral vectors are commonly employed for gene therapy. Chimeric viral vectors—such as hybrid vectors with qualities of both bacteriophages and eukaryotic viruses—have also been developed.
Viral vectors were first created in 1972 by Paul Berg. Further development was temporarily halted by a recombinant DNA research moratorium following the Asilomar Conference and stringent National Institutes of Health regulations. Once lifted, the 1980s saw both the first recombinant viral vector gene therapy and the first viral vector vaccine. Although the 1990s saw significant advances in viral vectors, clinical trials had a number of setbacks, culminating in Jesse Gelsinger's death. However, in the 21st century, viral vectors experienced a resurgence and have been globally approved for the treatment of various diseases. They have been administered to billions of patients, notably during the COVID-19 pandemic.

Characteristics

, infectious agents composed of a protein coat that encloses a genome, are the most numerous biological entities on Earth. As they cannot replicate independently, they must infect cells and hijack the host's replication machinery in order to produce copies of themselves. Viruses do this by inserting their genome—which can be DNA or RNA, either single-stranded or double-stranded—into the host. Some viruses may integrate their genome directly into that of the host in the form of a provirus.
This ability to transfer foreign genetic material has been exploited by genetic engineers to create viral vectors, which can transduce the desired transgene into a target cell. Viral vectors consists of three components:
  1. A protein capsid and sometimes an envelope that encapsidates the genetic payload. This determines the range of cell types that the vector infects, termed its tropism.
  2. A genetic payload: the transgene that results in the desired effect when expressed.
  3. A "regulatory cassette" that controls transgene expression, whether integrated into a host chromosome or as an episome. The cassette comprises an enhancer, a promoter, and auxiliary elements.

    Applications

Basic research

Viral vectors are routinely used in a basic research setting and can introduce genes encoding, for instance, complementary DNA, short hairpin RNA, or CRISPR/Cas9 systems for gene editing. Viral vectors are employed for cellular reprogramming, like inducing pluripotent stem cells or differentiating adult somatic cells into different cell types. Researchers also use viral vectors to create transgenic mice and rats for experiments. Viral vectors can be used for in vivo imaging via the introduction of a reporter gene. Further, transduction of stem cells can permit the tracing of cell lineage during development.

Gene therapy

seeks to modulate or otherwise affect gene expression via the introduction of a therapeutic transgene. Gene therapy by viral vectors can be performed by in vivo delivery by directly administering the vector to the patient, or ex vivo by extracting cells from the patient, transducing them, and then reintroducing the modified cells into the patient. Viral vector gene therapies may also be used for plants, tentatively enhancing crop performance or promoting sustainable production.
There are four broad categories of gene therapy: gene replacement, gene silencing, gene addition, or gene editing. Relative to other non-integrative gene therapy approaches, transgenes introduced by viral vectors offer multi-year long expression.

Vaccines

For use as vaccine platforms, viral vectors can be engineered to carry a specific antigen associated with an infectious disease or a tumor antigen. Conventional vaccines are not suitable for protection against some pathogens due to unique immune evasion strategies and differences in pathogenesis. Viral vector-based vaccines, for instance, could eventually offer immunity against HIV-1 and malaria.
While traditional subunit vaccines elicit a humoral response, viral vectors allow for intracellular antigen expression that activates MHC pathways via both direct and crosspresentation pathways. This induces a robust adaptive immune response. Viral vector vaccines also have intrinsic adjuvant properties via innate immune system activation and the expression of pathogen-associated molecular patterns, negating the need for any additional adjuvant. In addition to a more robust immune response in comparison to other vaccine types, viral vectors offer efficient gene transduction and can target specific cell types. Pre-existing immunity to the virus used as the vector, however, can be a significant issue.
Prior to 2020, viral vector vaccines were widely administered but confined to veterinary medicine. In the global response to the COVID-19 pandemic, viral vector vaccines played a fundamental role and were administered to billions of people, particularly in low and middle-income nations.

Types

Retroviruses

—enveloped RNA viruses—are popular viral vector platforms due to their ability to integrate genetic material into the host genome. Retroviral vectors comprise two general classes: gamma retroviral and lentiviral vectors. The fundamental difference between the two are that gamma retroviral vectors can only infect dividing cells, while lentiviral vectors can infect both dividing and resting cells. Notably, retroviral genomes are composed of single-stranded RNA and must be converted to proviral double-stranded DNA, a process known as reverse transcription—before it is integrated into the host genome via viral proteins like integrase.
The most commonly used gammaretroviral vector is a modified Moloney murine leukemia virus, able to transduce various mammalian cell types. MMLV vectors have been associated with some cases of carcinogenesis. Gammaretroviral vectors have been successfully applied to ex vivo hematopoietic stem cell to treat multiple genetic diseases.

Lentiviral vectors

Most lentiviral vectors are derived from human immunodeficiency virus type 1, although modified simian immunodeficiency virus, the feline immunodeficiency virus, and the equine infectious anaemia virus have also been utilized. As all functional genes are removed or otherwise mutated, the vectors are not cytopathic and can be engineered to be non-integrative.
Lentiviral vectors are able to carry up to 10 kb of foreign genetic material, although 3-4 kb was reported as optimal as of 2023. Relative to other viral vectors, lentiviral vectors possess the greatest transduction capacity, due to the formation of a three-stranded "DNA flap" during retro-transcription of the single-strand lentiviral RNA to DNA within the host.
Although largely non-inflammatory, lentiviral vectors can induce robust adaptive immune responses by memory-type cytotoxic T cells and T helper cells. This is largely due to lentiviral vectors' high tropism for dendritic cells, which activate T cells. However, they can infect all types of antigen-presenting cells. Moreover, as they are the only retroviral vectors able to efficiently transduce both dividing and non-dividing cells, make them the most promising vaccine platforms. They have also been trialed as vaccines against cancer.
Lentiviral vectors have been used as in vivo therapies, such as directly treating genetic diseases like haemophilia B and for ex vivo treatments like immune cell modification in CAR T cell therapy. In 2017, the US Food and Drug Administration approved tisagenlecleucel, a lentiviral vector, for acute lymphoblastic leukaemia.

Adenoviruses

Adenoviruses are double-stranded DNA viruses belonging to the family Adenoviridae. Their relatively large genomes, of approximately 30–45 kb, make them ideal candidates for genetic delivery; newer adenoviral vectors can carry up to 37 kb of foreign genetic material. Adenoviral vectors display high transduction efficiency and transgene expression, and can infect both dividing and non-dividing cells.
The adenoviral capsid, an icosahedron, features a fibre "knob" at each of its 12 vertices. These fibre proteins mediate cell entry—greatly affecting efficacy and contribute to its broad tropism—notably via coxsackie–adenovirus receptors. Adenoviral vectors can induce robust innate and adaptive immune responses. Its strong immunogenicity is particularly due to the transduction of dendritic cells, upregulating the expression of both MHC I and II molecules and activating the DCs. They have a strong adjuvant effect, as they display several pathogen-associated molecular patterns. One disadvantage is that pre-existing immunity to adenovirus serotypes is common, reducing efficacy. The use of chimpanzee adenoviruses may circumvent this issue.
While the activation of both innate and adaptive immune responses is an obstacle for many therapeutic applications, it makes adenenoviral vectors an ideal vaccine platform. The global response to the COVID-19 pandemic saw the development and use of multiple adenoviral vector vaccines, including Sputnik V, the Oxford–AstraZeneca vaccine, and the Janssen vaccine.