Single-molecule sequencing of an individual human genome
Single-molecule sequencing of an individual human genome is a scholarly work by Stephen Quake, published in 2009 in ''Nature Biotechnology''. The main subjects of the publication include personal genomics, genome, Molecular Inversion Probe, copy-number variation, RNA sequencing, single-nucleotide polymorphism, Sanger sequencing, Hybrid genome assembly, real-time polymerase chain reaction, Cancer genome sequencing, computational biology, genotyping, genetics, genomics, SNP genotyping, whole genome sequencing, indel, human genome, reference genome, DNA sequencing, and biology. The authors aligned billions of 24- to 70-bp reads (32 bp average) to approximately 90% of the National Center for Biotechnology Information (NCBI) reference genome, with 28x average coverage.