Manipulating Protein Conformations by Single-Molecule AFM-FRET Nanoscopy
Manipulating Protein Conformations by Single-Molecule AFM-FRET Nanoscopy is a scholarly work, published in 2012 in ''ACS Nano''. The main subjects of the publication include chemistry, Förster resonance energy transfer, crystallography, membrane raft, biophysics, cryogenic electron microscopy, nanotechnology, molecule, atomic force microscope, Single-molecule experiment, energy landscape, atomic force microscopy, Single-molecule FRET, fluorescence, force spectroscopy, and materials science. The authors have further demonstrated an application of using this nanoscopy on manipulation of single-molecule protein conformation and simultaneous single-molecule FRET measurement of a Cy3-Cy5-labeled kinase enzyme, HPPK (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase).