| Chapter | Charter date and range | Institution | Location | Status | |
| Alpha | – 1967 | University of Vermont College of Medicine | Burlington, Vermont | Inactive | |
| Alpha Alpha | – 1908 | Louisville Medical College | Louisville, Kentucky | Inactive | |
| Beta Beta | –1913 | Baltimore Medical College, University of Maryland | Baltimore, Maryland | Consolidated | |
| Beta First | – 1909 | Kentucky School of Medicine | Louisville, Kentucky | Consolidated, Reissued | |
| Gamma First | – 1907 | University of Louisville | Louisville, Kentucky | Consolidated, Reissued | |
| Delta First | – 1908 | Hospital College of Medicine | Louisville, Kentucky | Reissued | |
| Epsilon First | – 1907 | Kentucky University Medical Department | Lexington, Kentucky | Consolidated, Reissued | |
| Theta | – 1913 | University College of Medicine | Richmond, Virginia | Consolidated | |
| Gamma Gamma | – June 30, 1921 | Bowdoin Medical College | Brunswick, Maine | Inactive | |
| Delta Delta | – 1915 | University College of Physicians and Surgeons | Baltimore, Maryland | Consolidated | |
| Eta Second | – 1913 | Medical College of Virginia | Richmond, Virginia | Consolidated | |
| Kappa | – 1971 | Georgetown University School of Medicine | Washington, D.C. | Inactive | |
| Omicron | | Tulane University of Louisiana School of Medicine | New Orleans, Louisiana | Active | |
| Theta Theta | – 1907 | Maryland Medical College | Baltimore, Maryland | Consolidated | |
| Mu | – 1975 | Purdue University School of Medicine | Indianapolis, Indiana | Consolidated | |
| Nu | – June 1, 1913 | Birmingham Medical College | Tuscaloosa, Alabama | Consolidated | |
| Zeta | | University of Texas Medical Branch | Galveston, Texas | Active | |
| Chi | | Sidney Kimmel Medical College | Philadelphia, Pennsylvania | Active | |
| Iota | – 19xx ?; – 1963 | University of Alabama School of Medicine | Birmingham, Alabama | Inactive | |
| Phi | – 1978 | George Washington University Medical School | Washington, D.C. | Inactive | |
| Lambda | – June 1, 1911 | University of Pittsburgh School of Medicine | Pittsburgh, Pennsylvania | Inactive | |
| Pi | – 1976 | Vanderbilt University School of Medicine | Nashville, Tennessee | Inactive | |
| Rho | – 1944 | Rush Medical College | Chicago, Illinois | Inactive | |
| Sigma | – 1973 | Emory University School of Medicine | Atlanta, Georgia | Inactive | |
| Sigma Theta | – 1965 | University of North Carolina School of Medicine | Chapel Hill, North Carolina | Inactive | |
| Tau | – June 1, 1924 | Medical College of the State of South Carolina | Charleston, South Carolina | Reestablished | |
| Psi | | University of Michigan Medical School | Ann Arbor, Michigan | Active | |
| Upsilon | – 1917 | Atlanta School of Medicine | Atlanta, Georgia | Consolidated | |
| Alpha Theta | – 1941 | Western Reserve University | Cleveland, Ohio | Inactive | |
| Sigma Mu Chi | – June 1, 1910 | Chattanooga Medical College | Chattanooga, Tennessee | Inactive | |
| Gamma Epsilon | – 1909 | University of Kentucky | Lexington, Kentucky | Inactive | |
| Pi Sigma | – 1915 | University of Maryland | College Park, Maryland | Consolidated | |
| Xi First | – December 10, 1919 | Texas Christian University | Fort Worth, Texas | Moved | |
| Phi Sigma | – 1973; 1985–1989 | Stritch School of Medicine | Chicago, Illinois | Inactive | |
| Chi Theta | – October 1, 1917 | Medico-Chirurgical College of Philadelphia | Philadelphia, Pennsylvania | Consolidated | |
| Alpha Delta | 1908–1909 | Louisville and Hospital College | Louisville, Kentucky | Inactive | |
| Kappa Psi | – 1911 | College of Physicians & Surgeons | St. Louis, Missouri | Consolidated | |
| Upsilon Pi | – 1969 | University of Pennsylvania School of Medicine | Philadelphia, Pennsylvania | Inactive | |
| Pi Delta Phi First | – Fall 1911 | University of California, Los Angeles | Los Angeles, California | Moved | |
| Phi Beta | – 1916 | University of Illinois | Chicago, Illinois | Consolidated | |
| Psi Rho Sigma | – 1913 | Northwestern University School of Medicine | Chicago, Illinois | Reestablished | |
| Iota Pi | – June 1, 1920 | University of Southern California School of Medicine | Los Angeles, California | Reestablished | |
| Kappa Delta | – 1967 | Johns Hopkins University School of Medicine | Baltimore, Maryland | Inactive | |
| Alpha Mu | – 1917 | Indiana University School of Medicine | Bloomington, Indiana | Consolidated | |
| Theta Upsilon | – 1971 | Temple University School of Medicine | Philadelphia, Pennsylvania | Inactive | |
| Phi Rho | –2014 | Saint Louis University School of Medicine | Saint Louis, Missouri | Inactive | |
| Pi Delta Phi Second | – 1967 | University of California, Berkeley | Berkeley, California | Inactive | |
| Theta Eta | –1977 | Medical College of Virginia School of Medicine | Richmond, Virginia | Inactive | |
| Sigma Upsilon | – 1937 | Stanford University School of Medicine | San Francisco, California | Inactive | |
| Gamma Second | –1962 | Ohio State University College of Medicine | Columbus, Ohio | Inactive | |
| Delta Second | – 1957 | Tufts University School of Medicine | Boston, Massachusetts | Inactive | |
| Beta Second | – 19xx ?; –1931 | University of Oregon Medical School | Portland, Oregon | Inactive | |
| Epsilon Second | – 19xx ?; – 1942 | Wayne State University School of Medicine | Detroit, Michigan | Inactive | |
| Alpha Beta | | University of Tennessee Health Science Center | Memphis, Tennessee | Active | |
| Lambda Rho | – 19xx ?; – 1968 | University of Arkansas College of Medicine | Little Rock, Arkansas | Inactive | |
| Kappa Upsilon | – 1954 | University of Kansas Medical Center | Lawrence, Kansas | Consolidated | |
| Beta Delta | 1915 – 1950 | University of Maryland, Baltimore School of Medicine | Baltimore, Maryland | Inactive | |
| Chi Upsilon | | Creighton University School of Medicine | Omaha, Nebraska | Active | |
| Upsilon Nu | | University of Nebraska Medical School | Omaha, Nebraska | Active | |
| Upsilon Zeta | – 1971 | University of Cincinnati College of Medicine | Cincinnati, Ohio | Inactive | |
| Upsilon Iota | – 1952 | University of Illinois School of Medicine | Chicago, Illinois | Inactive | |
| Xi Second | – 192x ? | Baylor College of Medicine | Dallas, Texas | Reissused, Reestablished | |
| Kappa Rho | – 1971 | Northwestern University Medical School | Chicago, Illinois | Inactive | |
| Delta Pi | – 1945 | University of Utah School of Medicine | Salt Lake City, Utah | Inactive | |
| Kappa Chi | – 1974; 1981 | University of Minnesota Medical School | Minneapolis, Minnesota | Active | |
| Upsilon Sigma | – 1958 | Columbia University College of Physicians and Surgeons | New York, New York | Inactive | |
| Epsilon Chi | – 19xx ? | Marquette University | Milwaukee, Wisconsin | Inactive | |
| Rho Delta | – 1956 | Cornell University Medical School | New York, New York | Inactive | |
| Beta Chi | – 1937 | University of Colorado School of Medicine | Denver, Colorado | Inactive | |
| Tau Beta | – 1974 | University of Wisconsin Medical School | Madison, Wisconsin | Inactive | |
| Xi Third | – 1971 | University of Texas Southwestern Medical School | Dallas, Texas | Inactive | |
| Sigma Delta | – 1942 | University of South Dakota School of Medicine | Vermillion, South Dakota | Inactive | |
| Eta Upsilon | – 1935 | Harvard Medical School | Boston, Massachusetts | Inactive | |
| Beta Upsilon | – 1958 | Boston University School of Medicine | Boston, Massachusetts | Inactive | |
| Epsilon Delta | – 1939 | Washington University School of Medicine | St. Louis, Missouri | Inactive | |
| Beta Mu | – 1944 | McGill University Faculty of Medicine | Montreal, Canada | Inactive | |
| Pi Mu | – 1972 | University of Virginia School of Medicine | Charlottesville, Virginia | Inactive | |
| Omicron Kappa | – 1960 | University of Oklahoma College of Medicine | Oklahoma City, Oklahoma | Inactive | |
| Gamma Sigma | – 1943 | Yale School of Medicine | New Haven, Connecticut | Inactive | |
| Tau Omicron | – 1969 | University of Toronto Medical School | Toronto, Ontario, Canada | Inactive | |
| Mu Gamma | – 1951 | University of Iowa College of Medicine | Iowa City, Iowa | Inactive | |
| Theta Pi | 1926–1976 | University of Mississippi School of Medicine | Jackson, Mississippi | Inactive | |
| Sigma Kappa | | Medical University of South Carolina | Charleston, South Carolina | Active | |
| Nu Sigma | 1928–2009 | Dalhousie University Faculty of Medicine | Halifax, Nova Scotia, Canada | Inactive | |
| Delta Phi | 1929–1970 | University of Southern California School of Medicine | Los Angeles, California | Inactive | |
| Theta Beta Pi | 1929–1944 | Syracuse University College of Medicine | Syracuse, New York | Inactive | |
| Delta Kappa Upsilon | 1929–1940 | University of Kansas Medical Center | Kansas City, Kansas | Inactive | |
| Delta Upsilon | 1930–1978 | Duke University School of Medicine | Durham, North Carolina | Inactive | |
| Alpha Phi Sigma | 1931–1978 | Medical College of Georgia | Augusta, Georgia | Inactive | |
| Alpha Sigma | 1932–1975 | Louisiana State University Medical Center | New Orleans, Louisiana | Inactive | |
| Gamma Chi | 1933–1966 | West Virginia University School of Medicine | Morgantown, West Virginia | Inactive | |
| Omega Upsilon Phi | 1934–1973 | University at Buffalo School of Medicine and Biomedical Sciences | Buffalo, New York | Inactive | |
| Tau Kappa | 1935–1969 | Wake Forest School of Medicine | Winston-Salem, North Carolina | Inactive | |
| Alpha Mu | 1938–1960 | The State University of New York Health Science Center at Brooklyn | Brooklyn, New York | Inactive | |
| Xi Chi | 1943–1975 | Baylor College of Medicine | Houston, Texas | Inactive | |
| Phi Alpha | 1948–1978 | New York Medical College | Valhalla, New York | Inactive | |
| Phi Alpha Gamma | 1948–1964 | Hahnemann Medical College of Philadelphia | Philadelphia, Pennsylvania | Inactive | |
| Epsilon Kappa | | University of Washington School of Medicine | Seattle, Washington | Active | |
| Epsilon Beta | 1952–1967 | University of California Medical School | Los Angeles, California | Inactive | |
| Beta Alpha | 1953–1974 | University of Miami School of Medicine | Miami, Florida | Inactive | |
| Mu Sigma | 1955–1967 | University of Missouri School of Medicine | Columbia, Missouri | Inactive | |
| Omega | 1960–1968 | University of Mexico | Mexico City, Mexico | Inactive | |
| Upsilon Beta Second | 1962–1972 | University of Puerto Rico School of Medicine | San Juan, Puerto Rico | Inactive | |
| Phi Sigma Gamma | 1963–1967 | University of California, Los Angeles School of Medicine | Los Angeles, California | Inactive | |
| Upsilon Tau | 1970–1973 | University of Texas Health Science Center at San Antonio | San Antonio, Texas | Inactive | |
| Upsilon Alpha | 1981–1985 | University of Arizona College of Medicine | Tucson, Arizona | Inactive | |
| Sigma Chi Mu | | American University of the Caribbean School of Medicine | St. Maarten, Netherlands Antilles | Active | |
| Sigma Tau Chi | 2002–2010 | St. Christopher College of Medicine | Luton, England | Inactive | |
| Iota Mu | 2007 | St. George's University | Grenada West Indies | Active | |
| Mu Alpha | 2011 | Medical University of the Americas | Charlestown, Nevis | Active | |
| Sigma Omega | 2013 | St. Martinus University Faculty of Medicine | Willemstad, Curaçao | Active | |
| Mu Delta | 2014 | Avalon University School of Medicine | Willemstad, Curaçao | Active | |
| Rho Epsilon Omega | | Philadelphia College of Osteopathic Medicine, Georgia Campus | Suwanee, Georgia | Active | |
| Alpha Tau | | Saint James School of Medicine St. Vincent | Arnos Vale, Saint Vincent and the Grenadines | Active | |
| Delta Kappa | | Saint James School of Medicine Anguilla | Anguilla | Active | |