| Chapter | Charter date and range | Institution | Location | Region | Status | |
| Townshend | | Ohio State University | Columbus, Ohio | Northeast | Active | |
| Morrill | –1900; 1903 | Pennsylvania State University | University Park, Pennsylvania | Northeast | Active | |
| Morrow | | University of Illinois Urbana-Champaign | Urbana, Illinois | North Central | Active | |
| Cornell | | Cornell University | Ithaca, New York | Northeast | Active | |
| Kedzie | | Michigan State University | East Lansing, Michigan | North Central | Inactive | |
| Granite | | University of New Hampshire | Durham, New Hampshire | Northeast | Inactive | |
| Nebraska | | University of Nebraska–Lincoln | Lincoln, Nebraska | North Central | Active | |
| North Carolina | | North Carolina State University | Raleigh, North Carolina | Southeast | Active | |
| La Grange | –1973 | University of Minnesota | Saint Paul, Minnesota | North Central | Inactive | |
| Green Mountain | | University of Vermont | Burlington, Vermont | Northeast | Inactive | |
| [James James Wilson (Secretary of Agriculture)|Wilson (Secretary of Agriculture)|Wilson] | | Iowa State University | Ames, Iowa | North Central | Active | |
| Babcock | | University of Wisconsin–Madison | Madison, Wisconsin | North Central | Inactive | |
| Centennial | | Colorado State University | Fort Collins, Colorado | Western | Inactive | |
| Maine | | University of Maine | Orono, Maine | Northeast | Inactive | |
| Missouri | | University of Missouri | Columbia, Missouri | North Central | Inactive | |
| Elliott | | Washington State University | Pullman, Washington | Western | Inactive | |
| California | –1957 | University of California, Berkeley | Berkeley, California | Western | Inactive | |
| Purdue | | Purdue University | West Lafayette, Indiana | North Central | Active | |
| Kansas | | Kansas State University | Manhattan, Kansas | North Central | Active | |
| Dacotah | | North Dakota State University | Fargo, North Dakota | North Central | Inactive | |
| Scovell | | University of Kentucky | Lexington, Kentucky | Southeast | Inactive | |
| Morgan | | University of Tennessee | Knoxville, Tennessee | Southeast | Inactive | |
| Georgia | | University of Georgia | Athens, Georgia | Southeast | Inactive | |
| Louisiana | | Louisiana State University | Baton Rouge, Louisiana | Southeast | Inactive | |
| Oklahoma | | Oklahoma State University–Stillwater | Stillwater, Oklahoma | Southwest | Active | |
| Arkansas | | University of Arkansas | Fayetteville, Arkansas | South Central | Inactive | |
| Oregon | | Oregon State University | Corvallis, Oregon | Western | Inactive | |
| Maryland | | University of Maryland | College Park, Maryland | Northeast | Inactive | |
| Idaho | | University of Idaho | Moscow, Idaho | Western | Inactive | |
| Montana | | Montana State University | Bozeman, Montana | Western | Active | |
| Florida | | University of Florida | Gainesville, Florida | Southeast | Active | |
| Cook | | Rutgers University | New Brunswick, New Jersey | Northeast | Active | |
| West Virginia | | West Virginia University | Morgantown, West Virginia | Northeast | Inactive | |
| South Dakota | | South Dakota State University | Brookings, South Dakota | North Central | Active | |
| New Mexico | | New Mexico State University | Las Cruces, New Mexico | South Central | Inactive | |
| Arizona | | University of Arizona | Tucson, Arizona | Western | Inactive | |
| Mississippi | | Mississippi State University | Starkville, Mississippi | Southeast | Inactive | |
| South Carolina | | Clemson University | Clemson, South Carolina | Southeast | Inactive | |
| Virginia | | Virginia Tech | Blacksburg, Virginia | Southeast | Active | |
| Wyoming | | University of Wyoming | Laramie, Wyoming | Western | Inactive | |
| Rhode Island | | University of Rhode Island | Kingston, Rhode Island | Northeast | Inactive | |
| California Beta | –1961 | University of California, Los Angeles | Los Angeles, California | Western | Inactive | |
| Utah | | Utah State University | Logan, Utah | Western | Inactive | |
| California Gamma | | University of California, Davis | Davis, California | Western | Inactive | |
| Alabama | | Auburn University | Auburn, Alabama | Southeast | Inactive | |
| Delaware | | University of Delaware | Newark, Delaware | Northeast | Active | |
| Texas Alpha | | Texas A&M University | College Station, Texas | Southwest | Active | |
| Texas Beta | | Texas Tech University | Lubbock, Texas | Southwest | Active | |
| Connecticut | | University of Connecticut | Storrs, Connecticut | Northeast | Active | |
| Massachusetts | | University of Massachusetts Amherst | Amherst, Massachusetts | Northeast | Inactive | |
| California Delta | | California Institute of Technology | Pasadena, California | Western | Inactive | |
| Puerto Rico | | University of Puerto Rico at Mayagüez | Mayagüez, Puerto Rico | Southeast | Active | |
| Illinois Beta | | Southern Illinois University Carbondale | Carbondale, Illinois | North Central | Active | |
| California Epsilon | –xxxx?; 2005 | California State University, Fresno | Fresno, California | Western | Active | |
| Nevada | | University of Nevada, Reno | Reno, Nevada | Western | Inactive | |
| Arizona Beta | | Arizona State University | Tempe, Arizona | Western | Inactive | |
| Louisiana Beta | | Southwestern Louisiana University | Lafayette, Louisiana | South Central | Inactive | |
| California Zeta | | California Polytechnic State University | San Luis Obispo, California | Western | Inactive | |
| Illinois Gamma | | Western Illinois University | Macomb, Illinois | North Central | Active | |
| Utah Beta | | Brigham Young University | Provo, Utah | Western | Inactive | |
| Wisconsin Beta | | University of Wisconsin–Platteville | Platteville, Wisconsin | North Central | Active | |
| Texas Gamma | | Tarleton State University | Stephenville, Texas | Southwest | Active | |
| Wisconsin Gamma | | University of Wisconsin–River Falls | River Falls, Wisconsin | North Central | Active | |
| Illinois Delta | | Illinois State University | Normal, Illinois | North Central | Active | |
| Louisiana Gamma | | Louisiana Tech University | Ruston, Louisiana | Southwest | Active | |
| Tennessee Beta | | University of Tennessee at Martin | Martin, Tennessee | Southeast | Inactive | |
| Texas Delta | | West Texas A&M University | Canyon, Texas | South Central | Inactive | |
| Alabama Carver | | Alabama A&M University | Huntsville, AL | Southeast | Inactive | |
| Murray | | Murray State University | Murray, KY | Southeast | Inactive | |
| Mount Berry | | Berry College | Mount Berry, Georgia | Southeast | Active | |
| Oklahoma Beta | | Oklahoma Panhandle State University | Goodwell, Oklahoma | Southwest | Active | |
| Western Kentucky | | Western Kentucky University | Bowling Green, KY | South Central | Inactive | |
| California Eta | | California State University, Chico | Chico, California | Western | Active | |
| Louisiana Delta | | Southern University | Baton Rouge, LA | Southeast | Active | |
| Named | Chapter and year | Notability | |
| George Aiken | Green Mountain 1956 | horticulturist, Governor of Vermont, U.S. Senate | |
| Alfred Atkinson | Montana | agronomist, President of Montana State University, and president of University of Arizona | |
| Liberty Hyde Bailey | Cornell 1901 | Horticulturalist, founder of American Society for Horticultural Sciences, first dean of the College of Agriculture and Life Sciences at Cornell University. | |
| Robert C. Baker | Cornell 1943 | Inventor of the chicken nugget and professor emeritus of Food Science at Cornell University | |
| Leon Dexter Batchelor | Granite | horticulture professor and director of the University of California Citrus Experiment Station | |
| Ezra Taft Benson | 1953 | U.S. Secretary of Agriculture | |
| John Rusling Block | | U.S. Secretary of Agriculture | |
| Norman Borlaug | La Grange | Nobel laureate | |
| Edgar B. Brossard | La Grange | college professor; economist; and chair of the U.S. Tariff Commission | |
| Lester R. Brown | Cook | founder of the Earth Policy Institute | |
| Earl Butz | Purdue 1931 | U.S. Secretary of Agriculture | |
| Herman Cain | | former chairman and CEO of Godfather's Pizza | |
| Elbert N. Carvel | | Lieutenant Governor of Delaware and fertilizer manufacture | |
| Arthur B. Chapman | Elliott | Animal genetic researcher | |
| Walter Clore | Oklahoma | Father of the Washington wine industry | |
| John Eliot Coit | North Carolina | horticulture professor specializing in avocado, citrus and carob | |
| Dwight D. Eisenhower | Morrill | President of the United States | |
| Arthur Rose Eldred | Cornell 1916 | Agriculturalist, first Eagle Scout recognized by the Boy Scouts of America | |
| Orville Freeman | 1962 | Governor of Minnesota, U.S. Secretary of Agriculture | |
| Dan Glickman | | U.S. Secretary of Agriculture; president of the Motion Picture Association of America | |
| John A. Hannah | Kedzie | president of Michigan State College | |
| Clifford M. Hardin | Purdue 1953 | U.S. Secretary of Agriculture | |
| Otto Frederick Hunziker | Purdue | pioneer in the dairy industry, educator, and technical innovator | |
| William Marion Jardine | Kansas 1911 | U.S. Secretary of Agriculture, agronomist and president of Kansas State Agricultural College | |
| W. Pat Jennings | Virginia 1941 | United States House of Representatives | |
| Edwin Jackson Kyle | Cornell 1902 | U.S. Ambassador to Guatemala and namesake of Texas A&M's Kyle Field | |
| Jerry Litton | Missouri | United States House of Representatives | |
| Richard Lugar | Purdue | United States Senator | |
| Richard Lyng | Wilson 1974 | U.S. Secretary of Agriculture | |
| Al Maeder | La Grange | Professional football player | |
| Ron Micheli | Wyoming | director of the Wyoming Department of Agriculture and Wyoming House of Representatives | |
| Gerald A. Miller | Virginia | agronomist, professor, and associate dean emeritus at Iowa State University | |
| Henry A. Munger | Cornell 1936 | Vegetable breeder, professor and department chair Cornell University | |
| Robert B. Patterson | Mississippi | football player, planter, and founder of the first Citizens' Councils | |
| Stanley M. Powell | Kedzie | farmer and Michigan House of Representatives | |
| Roland Renne | Cook | economist and president of Montana State College - Bozeman | |
| Scott Rivkees | Cook | Surgeon General of Florida | |
| Nelson Rockefeller | Cornell 1962 | Governor of New York, Vice President of the United States | |
| Richard Rominger | California Davis | Deputy Secretary of Agriculture | |
| Henry P. Rusk | Missouri | dean of the Department of Agriculture, University of Illinois | |
| Albert Schatz | Cook | microbiologist and co-discoverer of streptomycin | |
| Robert W Scott | North Carolina | Governor of North Carolina | |
| Bob Sikes | Georgia | United States House of Representatives | |
| Mark G. Thornburg | Wilson | Secretary of Agriculture of Iowa | |
| Victor A. Tiedjens | Babcock | horticulturist, agronomist, biochemist, and soil chemist | |
| Selman Waksman | California Alpha | Nobel Prize in Physiology or Medicine, microbiologist, and co-discoverer of streptomycin | |
| Henry A. Wallace | Wilson 1908 | Vice President of the United States, U.S. Secretary of Agriculture, U.S. Secretary of Commerce | |
| Henry Cantwell Wallace | 1922 | U.S. Secretary of Agriculture | |
| Herbert John Webber | Nebraska | plant physiologist, professor, and first director of the University of California Citrus Experiment Station | |
| James Wilson | Wilson | U.S. Secretary of Agriculture, academic | |
| M. L. Wilson | Wilson 1907 | Agronomist, professor, and Undersecretary of the U.S. Department of Agriculture | |
| Kenneth E. Wing | Cornell 1958 | President of State University of New York at Cobleskill | |
| Dale E. Wolf | Nebraska | Lieutenant Governor of Delaware and temporary Governor of Delaware | |
| Clayton Yeutter | Nebraska 1950 | U.S. Secretary of Agriculture | |