| Airline | Image | IATA | ICAO | Callsign | Remarks |
| Ascend Airways | | YD | SYG | SYNERGY | Operates flights out of London Gatwick on behalf of TUI Airways and Air Sierra Leone. |
| Aer Lingus UK | | EG | EUK | GREEN FLIGHT | The Northern Irish subsidiary of the Irish flag carrier, Aer Lingus, based in Belfast. |
| British Airways | | BA | BAW / SHT | SPEEDBIRD / SHUTTLE | The flag carrier, with its hubs at Heathrow and Gatwick airports. It flies to 245 destinations all around the world, with a fleet of Airbus and Boeing aircraft. It is the second largest UK carrier behind easyJet. |
| BA CityFlyer | | CJ | CFE | FLYER | Regional airline, flying Embraer aircraft, it is a subsidiary of British Airways. Its head office is at Didsbury, Manchester, England; the airline operates all flights from its hub at London City Airport to UK and European destinations with the BA's full livery and flight numbers. |
| BA EuroFlyer | | A0 | EFW | GRIFFIN | Subsidiary of British Airways, operating flights from its hub at London Gatwick to short haul destinations with BA's full livery and flight numbers. |
| easyJet | | U2 | EZY | EASY | Low-cost carrier with its main hub at Luton Airport. It operates domestic and international scheduled services on 1,000 routes to 30 countries via its affiliate airlines, operating only Airbus aircraft. |
| Jet2.com | | LS | EXS | CHANNEX | Low-cost-leisure airline offering scheduled and charter flights from the country. With a fleet of Airbus and Boeing aircraft, it is the third largest airline in the UK, after easyJet and British Airways. |
| Loganair | | LM | LOG | LOGAN | Scottish regional airline with its base at Glasgow Airport near Paisley, Scotland. |
| Norse Atlantic UK | | Z0 | UBT | LONGBOAT | Low-cost carrier Long-haul; a subsidiary of Norse Atlantic Airways, its base at Gatwick Airport. |
| Ryanair UK | | RK | RUK | BLUE MAX | Low-cost carrier, a subsidiary of Ryanair, its base at London Stansted Airport. |
| Virgin Atlantic | | VS | VIR | VIRGIN | Long haul airline based in Crawley, England. |
| Wizz Air UK | | W9 | WUK | WIZZ GO | Low-cost carrier, a subsidiary of Wizz Air, with its base at Luton Airport. |
| Airline | Image | IATA | ICAO | Callsign | Remarks |
| Acropolis Aviation | | | CRV | ACROPOLIS | |
| AH Helicopters | | | | | |
| Air Charter Scotland | | | EDC | SALTIRE | Its call sign is a homage to the flag of Scotland. |
| Apollo Air Services | | | | | |
| Arena Aviation | | | REN | ARENA | No longer operating |
| Atlas Helicopters | | | TDT | TRIDENT | |
| Blu Halkin | | | | | |
| Babcock Mission Critical Services Offshore | | | ULR | VIPER | Originally known as Bond Helicopters. |
| Babcock Mission Critical Services Onshore | | | RHD | REDHEAD | |
| Bookajet | | | BOO | BOOKAJET | |
| British International Helicopter Services | | | BRT | BRITISH | |
| Capital Air Ambulance | | | EGL | PRESTIGE | |
| Capital Air Services | | | WDK | WOODSTOCK | |
| Cardinal Helicopter Services | | | | | |
| Castle Air | | | | CASTLEFILM | |
| Catreus AOC | | | VCG | THUNDER CAT | |
| Centreline Air Charter | | | CLF | CLIFTON | |
| CHC Scotia | | | HKS | HELIBUS | |
| Concierge U | | | JCO | CINCIERGE | |
| Corporate Jet Management | | | CJM | CHECKMARK | |
| Cutting Edge Helicopters | | | | | |
| DEA Aviation Limited | | | WKT | WHITE KNIGHT | |
| Directflight | | | DCT | AIRTASK | |
| Dragonfly Aviation Services | | | CBM | CAMBRIAN | |
| Eagle European | | | | | |
| East Midland Helicopters | | | CTK | COSTOCK | |
| EBG Helicopters | | | | REDHILL | Based at Redhill Aerodrome near London Gatwick Airport. |
| Elite Helicopters | | | | SOLENT | Based at Goodwood Airport, Chichester |
| Excel Charter | | | XEL | HELIEXCEL | |
| Execujet | | | LCY | LONDON CITY | Based at London City Airport |
| Executive Aviation Services | | | JTR | JESTER | |
| Flexjet Operations Limited | | | FLJ | FLEX AIR | Subsidiary of Flexjet LLC |
| Flight Academy Limited | | | EQT | EQUITY | |
| Flight Calibration Services Ltd | | | VOR | FLIGHTCAL | |
| FR Aviation | | | FRA | RUSHTON | Its call sign refers to its formation at RAF Tarrant Rushton. |
| Gama Aviation | | | GMA | GAMA | |
| GB Helicopters | | | EDY | SOLWAY | |
| Haverdfordwest Air Charter Services | | | PYN | POYSTON | |
| Hebridean Air Services | | | HBR | HEBRIDEAN | |
| Heli Air | | | HLY | WHISPER | |
| Helicentre Aviation | | | | | |
| Helicentre Liverpool | | | HLL | FUSION | |
| Helicopters Services | | | | | |
| HH Helicopters | | | HHH | CORINIUM | |
| IAS Medical | | | MDI | MEDOPS | |
| Isles of Scilly Skybus | | | IOS | SCILLONIA | |
| Jet Exchange | | | XCH | EXCHANGE | |
| London Executive Aviation | | | LNX | LONEX | |
| London Helicopter Centres | | | LHC | MUSTANG | No longer operating. |
| LyddAir | | | LYD | LYDDAIR | |
| Multiflight | | | MFT | YORKAIR | |
| Newcastle Aviation | | | APX | APEX | |
| NHV Helicopters | | | NHZ | GOLD | |
| North Wales Air Academy | | | | | |
| Northumbria Helicopters | | | NHL | NORTHUMBRIA | |
| Ortac AOC Ltd | | | ORT | SKYWALKER | Based in Jersey, CI |
| PDG Helicopters | | | PDG | OSPREY | |
| Pen-Avia | | | PDY | PENDLEY | |
| Phoenix Helicopter Academy | | | BPH | BLACK PHOENIX | |
| Polo Aviation | | | CUK | CHUKKA | Its call sign refers to a period in a game of horseback polo. |
| PremiAir Aviation Services | | | PGL | PREMIERE | |
| Ravenair | | | RVR | RAVEN | |
| Regency Jet Ltd. | | | LJC | LITTLE JET | |
| Rise Aviation | | | | | |
| Scillonia Airways | | | SCI | SCILLONIA | |
| Sloane Helicopters | | | SLN | SLOANE | |
| Sovereign Business Jets | | | RHK | REDHAWK | |
| Specialist Aviation Services | | | PLC | SPECIAL | |
| Starspeed | | | SSP | STARSPEED | |
| Synergy Aviation | | | SYG | SYNERGY | Rebranding as Ascend Airways. |
| Voluxis Limited | | | VXS | VOLUXIS | |
| Whizzard Helicopters | | | | SPELLCASTER | |
| Woodgate Aviation | | | CWY | CAUSEWAY | |
| Zenith Aviation | | | BZE | ZENSTAR | |
| Zephyr Aviation | | | MBG | CHALGROVE | |