| Club name | Home town | Kit
Manufacturer | Notes |
| Briobecca Urayasu | Urayasu, Chiba | Nike | |
| FC Imabari | Imabari, Ehime | Adidas | J.League 100 Year Plan club status holders
Promoted from Shikoku Soccer League after 1st place in 40th Regional Promotion Series |
| Honda FC | Hamamatsu, Shizuoka | Umbro | Defending champions of 2016 |
| Miyazaki, Miyazaki | Kappa | |
| Maruyasu Okazaki | Okazaki, Aichi | Penalty | |
| MIO Biwako Shiga | Kusatsu, Shiga | Nike | |
| Nara Club | All cities/towns in Nara | Squadra | J.League 100 Year Plan club status and J3 license holders |
| All cities/towns in Osaka | Mitre | |
| Aomori, Aomori | Under Armour | |
| Ryūgasaki, Ibaraki | Adidas | |
| Sony Sendai | Tagajō, Miyagi | Umbro | |
| Tokyo Musashino City | Musashino, Tokyo | Yonex | J.League 100 Year Plan club status holders |
| Tochigi, Tochigi | Mitre | J.League 100 Year Plan club status holders |
| Vanraure Hachinohe | Eastern cities/towns in Aomori | Athleta | J.League 100 Year Plan club status and J3 license holders |
| Veertien Mie | All cities/towns in Mie | Mizuno | Promoted from Tōkai Adult Soccer League Div. 1 after 2nd place in 40th Regional Promotion Series |
| Verspah Oita | Yufu & Ōita, Ōita | Warrix Sports | |
