Spur 421 is a former route of SH 16, and was designated on November 16, 1965, after SH 16 was rerouted around the west side of San Antonio along the Loop 410 freeway. The section of Spur 421 south of Callaghan Road was proposed for decommissioning in 2014 as part of TxDOT's San Antonio turnback proposal, which would have turned back over 129miles of roads to the city of San Antonio, but the city of San Antonio rejected that proposal.