Affolter began playing for Iowa in 2021–22. She played off the bench as Iowa reached the title game in the 2023 NCAA tournament. She joined the team's starting lineup in the 2024 postseason after an injury to guard Molly Davis. She recorded 11 points and 11 rebounds in Iowa's victory in the 2024 Big Ten title game and was recognized with all-tournament honors. In the 2024 NCAA Regional Final, she had 16 points and five rebounds, helping to lead the Hawkeyes to the Final Four and earning a spot on the All-Regional Team.