| Name | Mark | System | From | To | Successor | Notes |
| Air Line Railroad in South Carolina | | SOU | 1856 | 1870 | Atlanta and Richmond Air–Line Railway | - |
| Alcolu Railroad | ALU | | 1902 | 1936 | N/A | - |
| Asheville and Spartanburg Railroad | | SOU | 1881 | 1902 | Southern Railway – Carolina Division | - |
| Ashley River Railroad | | ACL | 1876 | 1901 | Savannah, Florida and Western Railway | - |
| Atlanta and Charlotte Air Line Railway | | SOU | 1877 | 1996 | Norfolk Southern Railway | - |
| Atlanta and Richmond Air–Line Railway | | SOU | 1870 | 1876 | South Carolina Air–Line Railway | - |
| Atlantic Coast Line Railroad | ACL | ACL | 1898 | 1967 | Seaboard Coast Line Railroad | - |
| Atlantic and French Broad Valley Railroad | | SOU | 1879 | 1882 | French Broad and Atlantic Railway | - |
| Atlantic, Greenville and Western Railway | | ACL | 1885 | 1887 | Carolina, Knoxville and Western Railway | - |
| Atlantic and Northwestern Railroad | | SOU | 1885 | 1887 | Georgia and Carolina Midland Railroad | - |
| Augusta and Edgefield Railroad | | SOU | 1884 | 1885 | Augusta, Edgefield and Newberry Railroad | - |
| Augusta, Edgefield and Newberry Railroad | | SOU | 1885 | 1887 | Georgia and Carolina Midland Railroad | - |
| Augusta and Knoxville Railroad | | ACL | 1880 | 1886 | Port Royal and Western Carolina Railway | - |
| Augusta, Knoxville and Greenwood Railroad | | ACL | | 1880 | Augusta and Knoxville Railroad | - |
| Augusta Northern Railway | | | 1912 | 1941 | N/A | - |
| Bamberg, Ehrhardt and Walterboro Railway | | | 1913 | 1938 | N/A | - |
| Barnwell Railway | | SOU | 1882 | 1891 | Carolina Midland Railway | - |
| Belton, Williamston and Easley Railroad | | SOU | 1878 | 1879 | Atlantic and French Broad Valley Railroad | - |
| Bennettsville and Cheraw Railroad | B&CW | | 1902 | 1950 | N/A | - |
| Bennettsville and Osborne Railroad | | | 1899 | 1902 | Bennettsville and Cheraw Railroad | - |
| Berkeley Railroad | | | 1892 | 1906 | N/A | - |
| Bishopville Railroad | | ACL | 1882 | 1892 | South and North Carolina Railroad | - |
| Blackville and Alston Railroad | | SOU | 1885 | 1886 | Blackville, Alston and Newberry Railroad | - |
| Blackville, Alston and Newberry Railroad | | SOU | 1886 | 1891 | Carolina Midland Railway | - |
| Blue Ridge Railroad of South Carolina | | SOU | 1852 | 1880 | Greenville and Columbia Railroad | - |
| Blue Ridge Railway | BLR | SOU | 1901 | | | - |
| Branchville and Bowman Railroad | | | 1890 | 1925 | N/A | - |
| Buffalo Union-Carolina Railroad | | | 1922 | 1971 | N/A | - |
| Cape Fear and Yadkin Valley Railway | | ACL | 1881 | 1898 | Wilmington and Weldon Railroad | - |
| Carolina, Atlantic and Western Railway | | SAL | 1914 | 1915 | Seaboard Air Line Railway | - |
| Carolina, Clinchfield and Ohio Railway of South Carolina | | ACL/ L&N | 1909 | 1990 | CSX Transportation | - |
| Carolina and Cumberland Gap Railway | | SOU | 1896 | 1898 | SOU | - |
| Carolina, Cumberland Gap and Chicago Railway | | SOU | 1882 | 1895 | Carolina and Cumberland Gap Railway | - |
| Carolina, Knoxville and Western Railway | | ACL | 1887 | 1899 | Charleston and Western Carolina Railway, Greenville and Knoxville Railway | - |
| Carolina Midland Railway | | SOU | 1891 | 1902 | Southern Railway – Carolina Division | - |
| Carolina Northern Railroad | | SAL | 1899 | 1905 | Raleigh and Charleston Railroad | - |
| Carolina and Northwestern Railway | CRN | SOU | 1982 | 1988 | Southern Railway | - |
| Carolina and Northwestern Railway | CR&N | SOU | 1900 | 1974 | Norfolk Southern Railway | - |
| Carolina Western Railroad | CARW | | 1923 | 1971 | Seaboard Coast Line Railroad | - |
| Carolina and Western Railroad | | | 1902 | 1916 | | - |
| Catawba Valley Railway | | SAL | 1906 | 1909 | Seaboard Air Line Railway | - |
| Central Railroad of South Carolina | | ACL | 1878 | | | Still exists as a lessor of CSX Transportation |
| Central of Georgia Railroad | CG | SOU | 1971 | 1972 | N/A | - |
| Charleston, Cincinnati and Chicago Railroad | | SOU | 1888 | 1893 | Ohio River and Charleston Railway | - |
| Charleston and Northern Railroad | | ACL | 1895 | 1895 | Cheraw and Darlington Railroad, Manchester and Augusta Railroad, Wilson and Summerton Railroad | - |
| Charleston Northern Railway | | SAL | 1913 | 1914 | North and South Carolina Railway | - |
| Charleston and Savannah Railroad | | ACL | 1853 | 1867 | Savannah and Charleston Railroad | - |
| Charleston and Savannah Railway | | ACL | 1880 | 1901 | Savannah, Florida and Western Railway | - |
| Charleston Southern Railway | | SAL | 1915 | 1915 | North and South Carolina Railway | - |
| Charleston, Sumter and Northern Railroad | | ACL | 1890 | 1895 | Charleston and Northern Railroad | - |
| Charleston Terminal Company | | ACL/ SOU | 1903 | 1921 | | - |
| Charleston Union Station Company | | ACL/ SOU | 1902 | 1970 | N/A | - |
| Charleston and Western Carolina Railway | C&WC, CWC | ACL | 1896 | 1959 | Atlantic Coast Line Railroad | - |
| Charlotte, Columbia and Augusta Railroad | | SOU | 1869 | 1894 | SOU | - |
| Charlotte, Monroe and Columbia Railroad | | SAL | 1901 | 1940 | N/A | - |
| Charlotte and South Carolina Railroad | | SOU | 1846 | 1869 | Charlotte, Columbia and Augusta Railroad | - |
| Cheraw and Chester Railroad | | | 1873 | 1896 | Lancaster and Chester Railway | - |
| Cheraw and Coalfields Railroad | | ACL | 1867 | 1868 | Cheraw and Salisbury Railroad | - |
| Cheraw and Darlington Railroad | | ACL | 1849 | 1898 | Atlantic Coast Line Railroad | - |
| Cheraw and Salisbury Railroad | | ACL | 1868 | 1882 | Cheraw and Darlington Railroad | - |
| Chester, Greenwood and Abbeville Railroad | | SAL | 1885 | 1886 | Georgia, Carolina and Northern Railway | - |
| Chester and Lenoir Narrow Gauge Railroad | | SOU | 1873 | 1897 | Carolina and Northwestern Railway | - |
| Chesterfield and Kershaw Railroad | | SAL | 1889 | 1901 | Seaboard Air Line Railway | - |
| Chesterfield and Lancaster Railroad | | SAL | 1887 | 1941 | N/A | - |
| Cincinnati and Charleston Railroad | | SOU | 1835 | 1836 | Louisville, Cincinnati and Charleston Railroad | - |
| Clinchfield Railroad | CRR | ACL/ L&N | 1924 | 1983 | Seaboard System Railroad | - |
| Colleton County Railroad | CCYR | | 1986 | 1988 | Hampton and Branchville Railroad | - |
| Columbia and Augusta Railroad | | SOU | 1863 | 1869 | Charlotte, Columbia and Augusta Railroad | - |
| Columbia and Greenville Railroad | | SOU | 1880 | 1894 | Blue Ridge Railway, Southern Railway | - |
| Columbia and Hamburg Railroad | | SOU | 1858 | 1863 | Columbia and Augusta Railroad | - |
| Columbia, Newberry and Laurens Railroad | CNL | ACL | 1886 | 1984 | Seaboard System Railroad | - |
| Columbia and Sumter Railroad | | ACL | 1866 | 1870 | Wilmington, Columbia and Augusta Railroad | - |
| Columbia Union Station Company | | ACL/ SOU | 1900 | 1971 | N/A | - |
| Conway Coast and Western Railroad | | ACL | 1904 | 1912 | Atlantic Coast Line Railroad | - |
| Conway Seashore Railroad | | ACL | 1899 | 1904 | Conway Coast and Western Railroad | - |
| Due West Railway | | | 1907 | 1940 | N/A | - |
| Duval Transportation of the Carolinas | | | 1987 | 1987 | Mid Atlantic Railroad | - |
| East Shore Terminal Company | | ACL/ SOU | 1887 | 1903 | Charleston Terminal Company | - |
| Eddy Lake and Northern Railroad | | | 1905 | | | - |
| Edgefield Branch Railroad | | SOU | 1878 | 1879 | Edgefield, Trenton and Aiken Railroad | - |
| Edgefield, Trenton and Aiken Railroad | | SOU | 1879 | 1882 | French Broad and Atlantic Railway | - |
| Edgemoor and Manetta Railway | EM | | 1902 | 1976 | N/A | - |
| Eutawville Railroad | | ACL | 1885 | 1890 | Charleston, Sumter and Northern Railroad | - |
| Florence Railroad | | ACL | 1882 | 1898 | Atlantic Coast Line Railroad | - |
| Florence and Fayetteville Railroad | | ACL | 1861 | 1881 | Cape Fear and Yadkin Valley Railway | - |
| Florida Central and Peninsular Railroad | | SAL | 1893 | 1900 | Seaboard Air Line Railway | - |
| French Broad and Atlantic Railway | | SOU | 1882 | 1882 | Carolina, Cumberland Gap and Chicago Railway | - |
| Georgetown and Lane's Railroad | | SAL | 1881 | 1887 | Georgetown and Western Railroad | - |
| Georgetown and North Carolina Railroad | | SOU | 1882 | 1885 | Charleston, Cincinnati and Chicago Railroad | - |
| Georgetown and North Carolina Narrow Gauge Railroad | | SOU | 1878 | 1882 | Georgetown and North Carolina Railroad | - |
| Georgetown and Western Railroad | | SAL | 1887 | 1915 | Carolina, Atlantic and Western Railway | - |
| Georgia and Carolina Midland Railroad | | SOU | 1887 | 1887 | Charleston, Cincinnati and Chicago Railroad | - |
| Georgia, Carolina and Northern Railway | | SAL | 1886 | 1901 | Seaboard Air Line Railway | - |
| Georgia and Florida Railroad | G&F | SOU | 1926 | 1963 | Georgia and Florida Railway | - |
| Georgia and Florida Railway | G&F, GF | SOU | 1963 | 1971 | Central of Georgia Railroad | - |
| Glenn Springs Railroad | | | 1894 | 1911 | N/A | - |
| Green Pond, Walterboro and Branchville Railroad | | ACL | 1900 | 1901 | Savannah, Florida and Western Railway | - |
| Green Pond, Walterboro and Branchville Railway | | ACL | 1887 | 1900 | Green Pond, Walterboro and Branchville Railroad | - |
| Greenville and Columbia Railroad | | SOU | 1845 | 1880 | Columbia and Greenville Railroad | - |
| Greenville and Knoxville Railroad | | | 1904 | 1914 | Greenville and Western Railway | - |
| Greenville and Laurens Railroad | | ACL | 1878 | 1886 | Port Royal and Western Carolina Railway | - |
| Greenville and Northern Railway | GRN | | 1920 | 1997 | Carolina Piedmont Railroad | - |
| Greenville and Port Royal Railroad | | ACL | 1882 | 1885 | Atlantic, Greenville and Western Railway | - |
| Greenville and Western Railroad | | | 1914 | 1920 | Greenville and Northern Railroad | - |
| Greenville and Western Railway | | | 1911 | 1969 | | - |
| Greenwood, Anderson and Western Railway | | SOU | 1895 | 1897 | Sievern and Knoxville Railroad | - |
| Greenwood, Laurens and Spartanburg Railroad | | ACL | 1880 | 1886 | Port Royal and Western Carolina Railway | - |
| Hampton and Branchville Railroad and Lumber Company | HB | | 1891 | 1924 | Hampton and Branchville Railroad | - |
| Hartsville Railroad | | ACL | 1884 | 1895 | Cheraw and Darlington Railroad | - |
| Horry County Railway | HCYR | | 1984 | 1987 | Waccamaw Coast Line Railroad | - |
| Kings Mountain Railroad | | SOU | 1848 | 1874 | Chester and Lenoir Narrow Gauge Railroad | - |
| Laurens Railroad | | ACL | 1847 | 1881 | Laurens Railway | - |
| Laurens Railway | | ACL | 1879 | 1895 | Columbia, Newberry and Laurens Railroad | - |
| Lockhart Railroad | | SOU | 1899 | | N/A | - |
| Louisville, Cincinnati and Charleston Railroad | | SOU | 1836 | 1843 | South Carolina Railroad | - |
| Manchester and Augusta Railroad | | ACL | 1870 | 1898 | Atlantic Coast Line Railroad | - |
| Marion County Railway | | | 1984 | 1985 | N/A | - |
| Marion and Southern Railroad | | SAL | 1907 | 1937 | N/A | - |
| Mid Atlantic Railroad | MRR | | 1987 | 1995 | Carolina Southern Railroad | - |
| Newberry and Laurens Railroad | | ACL | 1885 | 1886 | Columbia, Newberry and Laurens Railroad | - |
| Newberry, Whitmire and Augusta Railroad | | | 1906 | 1910 | Augusta Northern Railway | - |
| Norfolk Southern Railway | NS | SOU | 1974 | 1982 | Carolina and Northwestern Railway | - |
| North Charleston Terminal Company | | ACL/ SAL/ SOU | 1916 | | | Still exists as a joint subsidiary of CSX Transportation and the Norfolk Southern Railway |
| North and South Carolina Railway | | SAL | 1908 | 1914 | Carolina, Atlantic and Western Railway | - |
| Northeastern Railroad | | ACL | 1851 | 1898 | Atlantic Coast Line Railroad | - |
| Northwestern Railroad of South Carolina | | ACL | 1899 | 1935 | N/A | - |
| Ohio River and Charleston Railway | | SOU | 1894 | 1898 | South Carolina and Georgia Extension Railroad | - |
| Orangeburg Railway | | | 1911 | 1920 | N/A | - |
| Palmetto Railroad | | SAL | 1882 | 1895 | Palmetto Railway | - |
| Palmetto Railway | | SAL | 1895 | 1900 | Seaboard Air Line Railway | - |
| Pee Dee Bridge Company | | SAL | 1911 | 1915 | Carolina, Atlantic and Western Railway | - |
| Pickens Railroad | PICK | | 1892 | 1996 | Pickens Railway | - |
| Piedmont and Northern Railway | P&N | | 1911 | 1969 | Seaboard Coast Line Railroad | Electric until 1954 |
| Plant System | | ACL | 1894 | 1902 | Atlantic Coast Line Railroad | - |
| Port Royal Railroad | | ACL | 1856 | 1878 | Port Royal and Augusta Railway | - |
| Port Royal and Augusta Railway | | ACL | 1878 | 1896 | Charleston and Western Carolina Railway | - |
| Port Royal and Western Carolina Railway | | ACL | 1886 | 1896 | Charleston and Western Carolina Railway | - |
| Raleigh and Augusta Air Line Railroad | | SAL | 1878 | 1901 | Seaboard Air Line Railway | - |
| Raleigh and Charleston Railroad | | SAL | 1905 | 1941 | N/A | - |
| Raleigh and Gaston Railroad | | SAL | 1889 | 1900 | Seaboard Air Line Railway | - |
| Richmond and Danville Railroad | | SOU | 1879 | 1894 | Southern Railway | - |
| Rockingham Railroad | RKG | ACL | 1910 | 1968 | N/A | - |
| Rockton and Rion Railway | ROR | | 1932 | 1981 | N/A | - |
| Savannah and Charleston Railroad | | ACL | 1866 | 1880 | Charleston and Savannah Railway | - |
| Savannah, Florida and Western Railway | | ACL | 1901 | 1902 | Atlantic Coast Line Railroad | - |
| Savannah Valley Railroad | | ACL | | 1886 | Port Royal and Western Carolina Railway | - |
| Seaboard Air Line Railroad | SAL | SAL | 1946 | 1967 | Seaboard Coast Line Railroad | - |
| Seaboard Air Line Railway | | SAL | 1900 | 1945 | Seaboard Air Line Railroad | - |
| Seaboard Air Line System | | SAL | 1893 | 1900 | Seaboard Air Line Railway | - |
| Seaboard Coast Line Railroad | SCL | | 1967 | 1983 | Seaboard System Railroad | - |
| Seaboard and Roanoke Railroad | | SAL | 1889 | 1900 | Seaboard Air Line Railway | - |
| Seaboard System Railroad | SBD | | 1983 | 1986 | CSX Transportation | - |
| Sievern and Knoxville Railroad | | SOU | 1898 | 1933 | N/A | - |
| South Bound Railroad | | SAL | 1882 | 1901 | Seaboard Air Line Railway | - |
| South Carolina Railroad | | SOU | 1843 | 1881 | South Carolina Railway | - |
| South Carolina Canal and Railroad Company | | SOU | 1827 | 1844 | South Carolina Railroad | - |
| South Carolina Railway | | SOU | 1881 | 1894 | South Carolina and Georgia Railroad | - |
| South Carolina Air–Line Railway | | SOU | 1877 | 1877 | Atlanta and Charlotte Air Line Railway | - |
| South Carolina and Georgia Railroad | | SOU | 1894 | 1902 | Southern Railway – Carolina Division | - |
| South Carolina and Georgia Extension Railroad | | SOU | 1898 | 1902 | Southern Railway – Carolina Division | - |
| South Carolina Pacific Railway | | ACL | 1882 | 1984 | Seaboard System Railroad | - |
| South Carolina Terminal Company | | ACL/ SOU | 1893 | 1903 | Charleston Terminal Company | - |
| South Carolina Western Railway | | SAL | 1910 | 1914 | North and South Carolina Railway | - |
| South Carolina Western Extension Railway | | SAL | 1913 | 1914 | North and South Carolina Railway | - |
| South and North Carolina Railroad | | ACL | 1892 | 1896 | Manchester and Augusta Railroad | - |
| South and Western Railroad | | ACL/ L&N | 1908 | 1909 | Carolina, Clinchfield and Ohio Railway of South Carolina | - |
| Southern Railway | SOU | SOU | 1894 | 1990 | Norfolk Southern Railway | - |
| Southern Railway – Carolina Division | | SOU | 1902 | 1996 | Norfolk Southern Railway | - |
| Spartanburg and Asheville Railroad | | SOU | 1873 | 1881 | Asheville and Spartanburg Railroad | - |
| Spartanburg and Union Railroad | | SOU | 1847 | 1873 | Spartanburg, Union and Columbia Railroad | - |
| Spartanburg, Union and Columbia Railroad | | SOU | 1878 | 1896 | Asheville and Spartanburg Railroad | - |
| Sumter and Wateree River Railroad | | SOU | 1898 | 1902 | Southern Railway – Carolina Division | - |
| Union, Gaffney City and Rutherfordton Railroad | | SOU | 1878 | 1885 | Atlantic and Northwestern Railroad | - |
| Union and Glenn Springs Railroad | | | 1899 | 1922 | Buffalo Union-Carolina Railroad | - |
| Walterboro and Western Railway | | ACL | 1894 | 1900 | Green Pond, Walterboro and Branchville Railroad | - |
| Ware Shoals Railroad | WS | | 1908 | 1985 | N/A | - |
| Williamsburg Railroad | | ACL | 1876 | 1878 | Central Railroad of South Carolina | - |
| Wilmington and Carolina Railroad | | ACL | 1870 | 1870 | Wilmington, Columbia and Augusta Railroad | - |
| Wilmington, Chadbourn and Conway Railroad | | ACL | 1885 | 1895 | Wilmington and Conway Railroad | - |
| Wilmington, Columbia and Augusta Railroad | | ACL | 1870 | 1898 | Atlantic Coast Line Railroad | - |
| Wilmington and Conway Railroad | | ACL | 1895 | 1896 | Wilmington, Columbia and Augusta Railroad | - |
| Wilmington and Manchester Railroad | | ACL | 1846 | 1870 | Wilmington and Carolina Railroad | - |
| Wilmington and Weldon Railroad | | ACL | 1899 | 1900 | Atlantic Coast Line Railroad | - |
| Wilson and Summerton Railroad | | ACL | 1888 | 1899 | Northwestern Railroad of South Carolina | - |