Sf9 (cells)
Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells, are commonly used in insect cell culture for recombinant protein production using baculovirus expression system. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension.
Sf9 cell lines are widely utilized in the manufacture of recombinant protein antigens, including certain COVID-19 vaccines. Because subunit vaccines contain only specific viral components, they are generally considered safe for use across diverse demographic groups, including individuals with reduced immune function, as well as children and older adults. An additional practical advantage of this vaccine type is its stability, allowing for easier storage and distribution.