Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine
Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine is a scholarly work by Chun-Xiao Song and Tyson A Clark, published in 2011 in ''Nature Methods''. The main subjects of the publication include chemistry, 5-(hydroxymethyl)cytosine, deoxyribonucleic acid, AIDS, genomic DNA, sequencing by ligation, Single molecule real time sequencing, methylation, DNA sequencing, DNA nanoball sequencing, biology, epigenetics, Sequencing by hybridization, base pair, and computational biology. The authors describe strand-specific, base-resolution detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA with single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-hmC with single-molecule, real-time (SMRT) DNA sequencing.