List of highways numbered 27
Route 27, or Highway 27, may refer to:
Australia
- Burke Developmental Road
- Zeehan Highway
Canada
- Alberta Highway 27
- British Columbia Highway 27
- Manitoba Highway 27
- Prince Edward Island Route 27
- Saskatchewan Highway 27
- Ontario Highway 27
- * York Regional Road 27, formerly Highway 27 in York Region
- * Simcoe County Road 27, formerly Highway 27 in Simcoe County
Chile
- Chile Route 27
Costa Rica
- National Route 27
Croatia
- D27 road
Czech Republic
- part of I/27 Highway; Czech: :cz:Silnice I/27
France
- A27 autoroute
- Route nationale 27
Germany
- Bundesautobahn 27
- Bundesstraße 27
Greece
- A27 motorway, from Kozani to Ptolemaida and from Florina to Niki
- A27 road, a limited-access road from Ptolemaida to Florina
- EO27 road, from Amfissa to Bralos
India
- National Highway 27
Iran
- Road 27
Ireland
- N27 road
Italy
- Autostrada A27
Japan
- Japan National Route 27
- Maizuru-Wakasa Expressway
Korea, South
- Suncheon–Wanju Expressway
- National Route 27
Montenegro
- R-27 regional road
Netherlands
- A27 motorway
New Zealand
- New Zealand State Highway 27
Norway
- Norwegian County Road 27
Russia
- M27 highway
Spain
- Autovía A-27
Switzerland
- European route E27
United Kingdom
- British A27
- M27
United States
- Interstate 27
- U.S. Route 27
- Alabama State Route 27
- * County Route 27
- Arkansas Highway 27
- * Arkansas Highway 27B
- * Arkansas Highway 27N
- California State Route 27
- * County Route A27
- * County Route J27
- * County Route S27
- Connecticut Route 27
- Florida State Road 27
- Georgia State Route 27
- Idaho State Highway 27
- Illinois Route 27
- Iowa Highway 27
- K-27
- Louisiana Highway 27
- Maine State Route 27
- Maryland Route 27
- *Maryland Route 27B
- *Maryland Route 27C
- *Maryland Route 27D
- *Maryland Route 27E
- Massachusetts Route 27
- M-27
- Minnesota State Highway 27
- * County Road 27
- * County Road 27
- * County Road 27
- * County Road 27
- Mississippi Highway 27
- Missouri Route 27
- *Missouri Route 27
- Nebraska Highway 27
- * Nebraska Spur 27D
- * Nebraska Recreation Road 27B
- * Nebraska Recreation Road 27C
- Nevada State Route 27
- New Hampshire Route 27
- New Jersey Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- New Mexico State Road 27
- New York State Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- ** County Route 27B
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27B
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- * County Route 27
- North Carolina Highway 27
- North Dakota Highway 27
- Ohio State Route 27
- Oklahoma State Highway 27
- Oregon Route 27
- Pennsylvania Route 27
- South Carolina Highway 27
- South Dakota Highway 27
- Tennessee State Route 27
- Texas State Highway 27
- * Farm to Market Road 27
- * Texas Park Road 27
- Utah State Route 27
- Virginia State Route 27
- * Virginia State Route 27
- * Virginia State Route 27
- Washington State Route 27
- West Virginia Route 27
- Wisconsin Highway 27