PCR-based accurate synthesis of long DNA sequences
PCR-based accurate synthesis of long DNA sequences is a scholarly work, published in 2006 in ''Nature Protocols''. The main subjects of the publication include biology, DNA nanotechnology, cloning, primer dimer, DNA nanoball sequencing, G-quadruplex, molecular biology, DNA sequencing, sequencing by ligation, polymerase chain reaction, Sequencing by hybridization, primer, Multiple displacement amplification, oligonucleotide, computational biology, ribosome, biological sequence, In silico PCR, and deoxyribonucleic acid. The authors have successfully synthesized DNA fragments from 0.5 to 12.0 kb, with high G+C content, repetitive sequences or complex secondary structures.