Optimized Multicolor Immunofluorescence Panel


Optimized Multicolor Immunofluorescence Panel, abbreviation OMIP, was a series of standardized multi-color flow cytometry panel launched by International Society for Advancement of Cytometry, published in the form of columns in Cytometry Part A. The first two OMIP articles were published in September 2010 issue of this journal.

Purpose

The goals of OMIPs are:
  1. to alleviate the development time for researchers who wish to use the same panels
  2. to provide a starting point for the creation of novel OMIPs, and
  3. to provide a mechanism for attribution to the developers of the panel via citation of the publication.

List

No.No. of colorsPurposeSpeciesCell typesPaperYearCytometry
OMIP-00116T-cell cytokine production after in vitro stimulation and phenotyping of cytokine-producing T-cellsHumanPBMC2010BD LSR II
OMIP-00216Specificity and phenotype of CD8+ T-cellsHumanPBMC, bone marrow mononuclear cells2010BD LSR II
OMIP-00312Phenotyping memory B cellshumanPBMC, bone marrow, and tonsil mononuclear cell2011BD LSR II
OMIP-00415Determine Treg levels and to provide an in depth characterization of TregsHumanPBMC, ACK-lysed blood2012BD LSRFortessa
OMIP-00511T cell cytokine production and memory phenotypeRhesus macaquePBMC2012BD LSR II
OMIP-0069Enumerating and phenotyping of T regulatory cells and subsetsHumanPBMC2012BD LSR II
OMIP-00714Characterize the phenotype, maturation, and activation of NK cellsHumanPBMC2012-
OMIP-00811T cell cytokine production following in vitro stimulationHumanPBMC, T cell clones/lines, tumor-resident T cell lines2012-
OMIP-00910phenotype and function of CD4+ and CD8+ T-cellsHumanPBMC2012-
OMIP-01010immunophenotyping of small hematopoietic cellHumanCerebrospinal fluid, bone marrow, peripheral blood2012-
OMIP-0118Characterization of CECs in peripheral bloodHumanWhole blood, RBC-lysed and washed2012-
OMIP-0129Human leukocyte reconstitution and phenotypeHumanized mouseWhole blood, Ficoll-separated PBMC, splenocytes2012-
OMIP-01313T-cell differentiationHumanPBMC2012-
OMIP-01412T-cell cytokine production and function after in vitro stimulationHumanCryopreserved PBMC2012-
OMIP-01513T-reg characterization and T-cell activationHumanPBMC2013-
OMIP-01610Quality and phenotype of antigen-specific CD4 and CD8 T cellsCynomolgus macaque or HumanFresh or cryopreserved PBMCs2013-
OMIP-01713CD4+ helper T-cell subsets HumanPBMC2013-
OMIP-01815Phenotype of CD4 T cell subsets via chemokine receptor expressionHumanFresh PBMC, Bone marrow mononuclear cells2013-
OMIP-01914γδT-cells, iNKT-cells, haematopoietic precursorsHumanPBMC2013-
OMIP-02012γδ T-cell differentiation phenotypeHumanPBMC2014-
OMIP-02114NKT cells, γδ T-cells, MAIT cells, Memory T cell subsetsHumanPBMC2014-
OMIP-02214T-cell phenotyping, memory categorization, cytokine production, and function following in vitro stimulationHumanCryopreserved PBMC 2014-
OMIP-02310In-depth phenotyping of the main leukocyte subtypes in one runHumanFresh EDTA-treated peripheral blood, RBC-lysed and Fresh EDTA-treated Cord-blood2014-
OMIP-02418Characterize the phenotype of PBMC leukocyte subsetsHumanCryopreserved PBMC from adults and infants2014-
OMIP-02516Characterization of antigen-specific T cells, TFH-like cells and NK cellsHumanCryopreserved PBMC2014-
OMIP-02614Phenotype of B and plasma cellsRhesus macaque, African green monkeyFresh whole blood, PBMCs, mononuclear cells of bone marrow, lymph node, spleen, tonsil2015-
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OMIP-04919Subset analysis of hematopoiesis and hematopoietic diseaseHumanAny source containing human myeloid cells2018BD LSR II
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OMIP-06214Myeloid and innate/adaptive lymphoid comprehensive immunophenotypingHumanWhole peripheral blood, cord blood, PBMCs, nasal aspirate, and tracheal aspirate samples2019BD LSRFortessa
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OMIP-08225--
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OMIP-08428--
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OMIP-10127Broad immunophenotyping of leukocytes including myeloid and lymphoid cell lineages HumanFresh or fixed, cryopreserved whole blood2024BD FACSymphony A5