| Institution | Location | Founded | Affiliation | Enrollment | Nickname | Joined | Left | Primary conference |
| Chatham University | Pittsburgh, Pennsylvania | 1869 | Nonsectarian | 2,300 | Cougars | 2017 | 2018 | Presidents' (PAC) |
| Defiance College | Defiance, Ohio | 1850 | United Church of Christ | 505 | Yellow Jackets | 2014 | 2015 | Wolverine–Hoosier (WHAC) |
| Earlham College | Richmond, Indiana | 1847 | Quaker | 612 | Quakers | 2017 | 2018 | Heartland (HCAC) |
| Franklin College | Franklin, Indiana | 1834 | Baptist | 1,047 | Grizzlies | 2014 | 2018 | Heartland (HCAC) |
| Hanover College | Hanover, Indiana | 1827 | Presbyterian | 1,068 | Panthers | 2014 | 2018 | Heartland (HCAC) |
| Mount St. Joseph University | Delhi Township, Ohio | 1920 | Catholic
| 1,889 | Lions | 2014 | 2018 | Heartland (HCAC) |
| Saint Vincent College | Latrobe, Pennsylvania | 1846 | Catholic
| 1,652 | Bearcats | 2014 | 2018 | Presidents' (PAC) |
| Thiel College | Greenville, Pennsylvania | 1866 | Lutheran ELCA | 790 | Tomcats | 2014 | 2018 | Presidents' (PAC) |
| Thomas More University | Crestview Hills, Kentucky | 1921 | Catholic
| 1,900 | Saints | 2014 | 2018 | Great Midwest (G-MAC) |
| Transylvania University | Lexington, Kentucky | 1780 | Disciples of Christ | 963 | Pioneers | 2014 | 2018 | Heartland (HCAC) |
| Washington & Jefferson College | Washington, Pennsylvania | 1781 | Nonsectarian | 1,519 | Presidents | 2014 | 2018 | Presidents' (PAC) |
| Waynesburg University | Waynesburg, Pennsylvania | 1849 | Presbyterian | 1,500 | Yellow Jackets | 2014 | 2018 | Presidents' (PAC) |
| Westminster College | New Wilmington, Pennsylvania | 1852 | Presbyterian | 1,482 | Titans | 2016 | 2018 | Presidents' (PAC) |