Mid-South Conference (MHSAA)
The Mid-South Conference is an athletic conference affiliated with the MHSAA. All schools are Class D. Members are located in Michigan.
Full members
''Note: enrollments taken from 2020-21 MHSAA enrollment listing ''Past members
| Institution | Location | Affiliation | Nickname | Class | Enrollment | Colors | Left Conference |
| Washtenaw Christian Academy | Saline, Michigan | Private | Wildcats | D | 51 | 2008 | |
| Hillsdale Academy | Hillsdale, Michigan | Private | Colts | D | 67 | 2009 | |
| Holt Lutheran School* | Holt, Michigan | Private/Lutheran | Hawks | D | 25 | 2012 |
''*It is currently unclear if Holt Lutheran is in the conference due to major staff changes and low enrollment in their athletic programs, because of this they haven't been able to fully participate in most varsity sports.''