The species is a ground-dwelling spider that appears to be restricted to conditions of high humidity and relatively even temperature as found in the understory and litter layer of indigenous forest and close-canopy bush. Microstigmata longipes has also been sampled by splitting open damp, rotting logs. From surveys at the Ngome State Forest near Vryheid, M. longipes was more active in indigenous forest compared to pine plantations. The species is active throughout the year with the lowest numbers recorded in winter, with males peaking in November. It has been sampled from the Forest, Savanna, and Indian OceanCoastalBeltbiomes at altitudes ranging from 5 to 2,785 m above sea level.
Microstigmata longipes was originally described by Reginald Frederick Lawrence in 1938 as Microstigma longipes from the Umkomaas Valley in KwaZulu-Natal. The species was subsequently revised by Griswold in 1985.