Mechanistic insights on the Dicer-independent AGO2-mediated processing of AgoshRNAs
Mechanistic insights on the Dicer-independent AGO2-mediated processing of AgoshRNAs is a scholarly work by Alex Harwig, Elena Herrera-Carrillo, Ben Berkhout, and Ying Poi Liu, published in 2015 in ''RNA Biology''. The main subjects of the publication include Argonaute protein family, gene silencing, small interfering RNA, Ribonuclease III, Dicer 1, ribonuclease III, computational biology, Drosha ribonuclease III, Small hairpin RNA, microRNA, RNA interference, gene knockdown, cell biology, RNA splicing, ribonucleic acid, effector, and biology. The authors demonstrate by deep sequencing that AGO2-processed AgoshRNAs produce RNA effector molecules with more discrete ends than the products of the regular shRNA design.