Masters W35 400 metres world record progression
This is the progression of world record improvements of the 400 metres W35 division of Masters athletics.
;Key:
| Hand | Auto | Athlete | Nationality | Birthdate | Location | Date |
| 49.46 | Allyson Felix |  United States |
This is the progression of world record improvements of the 400 metres W35 division of Masters athletics.
;Key: