| Chapter | Charter date and range | Institution | Location | Status | |
| Alpha | – 1912 | Wesleyan University | Middletown, Connecticut | Withdrew | |
| Beta | 1872–1925 | Syracuse University | Syracuse, New York | Inactive | |
| Gamma | 1874–1920, 1925-1940 | Union College | Schenectady, New York | Inactive | |
| Delta | 1877–1905 | Cornell University | Ithaca, New York | Inactive | |
| Epsilon | 1878–1908 | University of Rochester | Rochester, New York | Inactive | |
| Zeta | 1879–1912 | University of California | Berkeley, California | Withdrew | |
| Eta | 1880–1923 | Colgate University | Hamilton, New York | Inactive | |
| Theta | 1881–1915 | Kenyon College | Gambier, Ohio | Inactive | |
| Iota | 1881–1921 | Western Reserve University | Cleveland, Ohio | Inactive | |
| Kappa | 1882–1912 | Hamilton College | Kirkland, New York | Inactive | |
| Lambda | 1881–1940 | Rensselaer Polytechnic Institute | Troy, New York | Merged | |
| Lambda Deuteron | 1882–1908 | Williams College | Williamstown, Massachusetts | Inactive | |
| Mu | 1883–1934 | Stevens Institute of Technology | Hoboken, New Jersey | Inactive | |
| Nu | 1884–1925 | Lafayette College | Easton, Pennsylvania | Inactive | |
| Beta Beta | 1885–1914 | Ohio Wesleyan University | Delaware, Ohio | Inactive | |
| Xi | 1885–1904 | Amherst College | Amherst, Massachusetts | Inactive | |
| Omicron | 1887–1924 | Allegheny College | Meadville, Pennsylvania | Inactive | |
| Omicron Deuteron | 1887–1907 | Lehigh University | Bethlehem, Pennsylvania | Inactive | |
| Pi Pi | 1887–1925 | Dickinson College | Carlisle, Pennsylvania | Inactive | |
| Pi | 1888–1903, 1927–1940 | Pennsylvania State College | State College, Pennsylvania | Inactive | |
| Rho | 1888–1919 | University of Pennsylvania | Philadelphia, Pennsylvania | Reestablished | |
| Sigma | 1889–1914 | New York University | New York City, New York | Inactive | |
| Pi Chi | 1890–1906 | University of Toronto | Toronto, Ontario, Canada | Inactive | |
| Psi Omega | 1890–1909 | University of the South | Sewanee, Tennessee | Inactive | |
| Tau | 1891–1910 | University of Wooster | Wooster, Ohio | Inactive | |
| Beta Lambda | 1891–1911 | Mount Union College | Alliance, Ohio | Inactive | |
| Phi | 1892–1914 | Rutgers University | New Brunswick, New Jersey | Withdrew | |
| Upsilon | 1892–1916 | University of Michigan | Ann Arbor, Michigan | Inactive | |
| Chi | 1893–1925 | Dartmouth College | Hanover, New Hampshire | Inactive | |
| Psi | 1893–1914 | Ohio State University | Columbus, Ohio | Reestablished | |
| Psi Deuteron | –19xx ? | Northwestern University | Evanston, Illinois | Inactive | |
| Alpha Psi | 1893–1905 | University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | Inactive | |
| Alpha Alpha | 1894–1913 | Bowdoin College | Brunswick, Maine | Inactive | |
| Delta Sigma | 1894–1925 | University of Kansas | Lawrence, Kansas | Inactive | |
| Alpha Gamma | 1914–1930 | Duke University | Durham, North Carolina | Inactive | |
| Alpha Delta | 1893–1925 | Illinois Wesleyan University | Bloomington, Illinois | Inactive | |
| Alpha Sigma | 1890–1912 | Washington and Lee University | Lexington, Virginia | Inactive | |
| Alpha Eta | 1894–1914 | University of Nebraska | Lincoln, Nebraska | Reestablished | |
| Omega | 1894–1913 | Swarthmore College | Swarthmore, Pennsylvania | Inactive | |
| Delta Delta | 1895–1923 | University of Maine | Orono, Maine | Withdrew | |
| Epsilon Epsilon | 1896–1913 | Case School of Applied Science | Cleveland, Ohio | Inactive | |
| Alpha Theta | 1895–1924, 2003–c. 2008 | University of Missouri | Columbia, Missouri | Inactive | |
| Alpha Iota | 1895–1913, 1924–1928 | Harvard University | Cambridge, Massachusetts | Inactive | |
| Beta Deuteron | 1895–xxxx ? | Hampden–Sydney College | Hampden Sydney, Virginia | Inactive | |
| Alpha Pi | 1895-1908 | Middlebury College | Middlebury, Vermont | Inactive | |
| Delta Rho | 1895–1923 | Northwestern University | Evanston, Illinois | Inactive | |
| Delta Tau | 1895–1923 | University of Chicago Professional Education | Chicago, Illinois | Inactive | |
| Pi Phi | 1895–1910 | University of Virginia | Charlottesville, Virginia | Inactive | |
| Mu Epsilon | 1896–1902 | Washington & Jefferson College | Washington, Pennsylvania | Inactive | |
| 1896–1897 | Wittenberg University | Springfield, Ohio | Inactive | |
| Gamma Kappa | 1897–1902 | City University of New York | New York City, New York | Inactive | |
| Gamma Xi | 1897–1902 | Bethany College | Bethany, West Virginia | Inactive | |
| Zeta Zeta | 1897–1912 | Roanoke College | Salem, Virginia | Inactive | |
| Gamma Gamma | 1898–1910 | Trinity College | Hartford, Connecticut | Inactive | |
| Alpha Zeta | 1898-1925 | University of Vermont | Burlington, Vermont | Inactive | |
| Kappa Gamma | 1898–1914 | University of Vermont Medical School | Burlington, Vermont | Inactive | |
| Alpha Rho | 1898–xxxx ? | University of South Carolina | Columbia, South Carolina | Reestablished | |
| Alpha Rho Deuteron | 1898–1901 | Central University of Kentucky | Richmond, Kentucky | Moved | |
| Kappa Beta | 1899–1929 | Bucknell University | Lewisburg, Pennsylvania | Inactive | |
| 1899–1905 | Susquehanna University | Selinsgrove, Pennsylvania | Inactive | |
| 1899–1940 | Washington University in St. Louis | St. Louis, Missouri | Inactive | |
| Alpha Nu | 1900–1907 | Tulane University | New Orleans, Louisiana | Inactive | |
| Alpha Upsilon | 1900–1912 | University of Kentucky | Lexington, Kentucky | Inactive | |
| 1900–1905 | Baylor University | Waco, Texas | Inactive | |
| Beta Upsilon | 1900–1912 | Brown University | Providence, Rhode Island | Inactive | |
| Princeton | 1900–1902 | Princeton University | Princeton, New Jersey | Inactive | |
| 1900–1923 | University of Pittsburgh | Pittsburgh, Pennsylvania | Inactive | |
| 1900–1900, 1948–xxxx ? | University of Nevada | Reno, Nevada | Inactive | |
| 1900–1900 | University of Cincinnati | Cincinnati, Ohio | Inactive | |
| 1900–1923 | Bates College | Lewiston, Maine | Inactive | |
| Alpha Rho Deuteron | 1901–1940 | Centre College | Danville, Kentucky | Inactive | |
| Delta Epsilon | 1901–1924 | Indiana University | Bloomington, Indiana | Inactive | |
| Alpha Xi | 1901–1911 | Oglethorpe University | Brookhaven, Georgia | Inactive | |
| Alpha Omicron | 1901–1910 | Cumberland University | Lebanon, Tennessee | Inactive | |
| Alpha Omega | 1901–1914 | Columbia University | New York City, New York | Inactive | |
| Invictus | 1901–1923, 1923–1994, 2008–20xx ? | Vanderbilt University | Nashville, Tennessee | Inactive | |
| Sigma Alpha | 1902–1910 | Westminster College | New Wilmington, Pennsylvania | Inactive | |
| Beta Eta | 1902–1906 | Muhlenberg College | Allentown, Pennsylvania | Inactive | |
| Alpha Epsilon | 1902–1913 | University of Colorado | Boulder, Colorado | Inactive | |
| Alpha Rho | 1902–1914, 19xx ?–1940s | University of Alabama | Tuscaloosa, Alabama | Withdrew | |
| Alpha Tau | 1902–1913 | Iowa Wesleyan University | Mount Pleasant, Iowa | Inactive | |
| Beta Zeta | 1902–1904 | Nebraska Wesleyan University | Lincoln, Nebraska | Inactive | |
| Omicron Omicron | 1903–1905, 1923–1942 | Ohio Northern University | Ada, Ohio | Merged | |
| Pi Pi | 1903–1925 | Dickinson College of Law | Carlisle, Pennsylvania | Inactive | |
| Beta Nu | 1903–1910 | University of South Carolina | Columbia, South Carolina | Inactive | |
| Beta Omicron | 1903–1914 | Colby College | Waterville, Maine | Inactive | |
| Lambda Sigma | 1903–1915 | Yale University | New Haven, Connecticut | Inactive | |
| Beta Mu | 1904–1909 | Emory University | Atlanta, Georgia | Inactive | |
| Beta Xi | 1904–1914 | DePauw University | Greencastle, Indiana | Inactive | |
| Beta Psi | 1904–1913 | University of Florida | Gainesville, Florida | Inactive | |
| Omega Nu | 1904–1914 | University of Maryland | College Park, Maryland | Inactive | |
| Zeta Phi | 1904–1914 | Boston University | Boston, Massachusetts | Inactive | |
| Zeta Phi | 1904–1914 | Massachusetts Institute of Technology | Cambridge, Massachusetts | Inactive | |
| Sigma Rho | 1904–1913 | University of Georgia | Athens, Georgia | Inactive | |
| 1904–1940 | Kentucky State University | Frankfort, Kentucky | Inactive | |
| Sigma Tau | 1904–1923 | University of Maryland Medical College | Baltimore, Maryland | Inactive | |
| Omega Kappa | 1905–1913 | Baltimore Medical College | Baltimore, Maryland | Inactive | |
| Alpha Phi | 1906–1912 | University of Mississippi | Oxford, Mississippi | Inactive | |
| Kappa Kappa | 1907–1914 | University of Texas | Austin, Texas | Withdrew | |
| Psi Psi | 1907–19xx ?, 1930–1935 | Iowa State University | Ames, Iowa | Inactive | |
| Alpha Delta | 1907–1925 | Illinois Wesleyan University | Bloomington, Illinois | Inactive | |
| Kappa Rho | 1907–1925 | Baltimore College of Dental Surgery | Baltimore, Maryland | Inactive | |
| 1908–1912 | Randolph–Macon College | Ashland, Virginia | Inactive | |
| Beta Pi | 1908–1914 | Davidson College | Davidson, North Carolina | Inactive | |
| Sigma Phi | 1908–1918 | University of Tennessee College of Medicine | Memphis, Tennessee | Inactive | |
| Gamma Beta | 1909–1928 | Jefferson Medical College | Philadelphia, Pennsylvania | Inactive | |
| 1909–1925, 1932–1939 | George Washington University | Washington, D.C. | Inactive | |
| Epsilon Deuteron | 1909–1921 | University of Rochester Alumni | Rochester, New York | Inactive | |
| Nu Mu | 1909–1913 | Auburn University | Auburn, Alabama | Inactive | |
| 1909–1923 | University of Washington | Seattle, Washington | Inactive | |
| 1909–1929 | University of Denver | Denver, Colorado | Inactive | |
| Alpha Alpha | 1910–1923 | Purdue University | West Lafayette, Indiana | Inactive | |
| Zeta Zeta | 1910–1917 | University of Wyoming | Laramie, Wyoming | Inactive | |
| Eta Eta | 1910–1914 | University of Massachusetts | Amherst, Massachusetts | Inactive | |
| Iota Iota | 1910–1915 | University of Wisconsin | Madison, Wisconsin | Inactive | |
| Rho Rho | 1910–1913 | Norwich University | Northfield, Vermont | Inactive | |
| 1910–1923, 1928–1928, 1934–1946 | University of Oregon | Eugene, Oregon | Inactive | |
| 1910–1914 | Niagara University | Lewiston, New York | Inactive | |
| 1910–1912 | Gettysburg College | Gettysburg, Pennsylvania | Inactive | |
| 1910–1912 | Franklin & Marshall College | Lancaster, Pennsylvania | Inactive | |
| 1910–1923 | Colorado School of Mines | Golden, Colorado | Inactive | |
| Omega Omega | 1910–1915 | Georgia Tech | Atlanta, Georgia | Inactive | |
| Alpha Chi | 1910–1924 | University of Illinois | | Inactive | |
| Omicron Omega | 1910–1916 | St. Lawrence University | Canton, New York | Inactive | |
| Theta Theta | 1911–1914 | West Virginia University | Morgantown, West Virginia | Inactive | |
| Mu Mu | 1911–1914 | Stanford University | Stanford, California | Inactive | |
| Nu Nu | 1911–1931, 1929–1931 | Marquette University | Milwaukee, Wisconsin | Inactive | |
| Xi Xi | 1911–19xx ?, 1928–1933 | University of Louisville | Louisville, Kentucky | Inactive | |
| Sigma Sigma | 1911–1915 | Medical College of Virginia | Richmond, Virginia | Inactive | |
| 1911–19xx ? | Denison University | Granville, Ohio | Inactive | |
| 1912–1940 | Johns Hopkins University | Baltimore, Maryland | Inactive | |
| Upsilon Upsilon | 1912–1940 | New York University Washington Square | Manhattan, New York | Inactive | |
| Phi Phi | 1912–1925 | University of Arkansas | Fayetteville, Arkansas | Inactive | |
| Chi Chi | 1913–1925 | University of Iowa | Iowa City, Iowa | Inactive | |
| Tau Tau | 1914–1925 | Baker University | Baldwin City, Kansas | Inactive | |
| Alpha Beta | 1914–1918, 1925–1928 | University at Buffalo | Buffalo, New York | Inactive | |
| Alpha Gamma | 1914–1924 | Duke University | Durham, North Carolina | Inactive | |
| Alpha Epsilon | 1915–1925 | University of South Dakota | Vermillion, South Dakota | Inactive | |
| Alpha Eta | 1916–1925 | Rush Medical College | Chicago, Illinois | Inactive | |
| Alpha Kappa | 1916–1923 | Northwestern University Dental School | Evanston, Illinois | Inactive | |
| 1916–1929 | Oregon State University | Corvallis, Oregon | Inactive | |
| Alpha Lambda | 1917–1925 | University of Nebraska | Lincoln, Nebraska | Inactive | |
| Beta Gamma | 1921–1925 | Chicago College of Dental Surgery | Chicago, Illinois | Inactive | |
| Beta Epsilon | 1917–1925 | Oklahoma State University | Stillwater, Oklahoma | Inactive | |
| Delta Iota | 1919–1925 | University of Illinois Professional Schools | | Inactive | |
| Delta Beta | 1922–1925 | Lehigh University | Bethlehem, Pennsylvania | Inactive | |
| Beta Delta | 1921–1925 | University of Chicago | Chicago, Illinois | Inactive | |
| Beta Iota | 1921–1924 | Drake University | Des Moines, Iowa | Inactive | |
| Beta Kappa | 1921–1923 | Millikin University | Decatur, Illinois | Inactive | |
| Beta Sigma | 1921–1925 | Kansas State University | Manhattan, Kansas | Inactive | |
| Beta Chi | 1921–1924 | University of Minnesota | Minneapolis, Minnesota | Inactive | |
| Delta Chi | 1922–1925 | University of Oklahoma | Norman, Oklahoma | Inactive | |
| c. 1922–c. 1955 | University of Oregon | Eugene, Oregon | Inactive | |
| 1923–1929 | Colorado State University | Fort Collins, Colorado | Inactive | |
| 1923–1923 | University of Northern Colorado | Greeley, Colorado | Inactive | |
| Chi Alpha | 1923–1949; October 25, 2005 – 20xx ? | University of New Mexico | Albuquerque, New Mexico | Inactive | |
| 1923–1940 | Colorado College | Colorado Springs, Colorado | Inactive | |
| 1923–1940 | William Jewell College | Liberty, Missouri | Inactive | |
| 1923–1940 | Michigan State University | East Lansing, Michigan | Inactive | |
| 1923–1940 | Washington State University | Pullman, Washington | Inactive | |
| Delta Lambda | 1923–1928 | Kansas City Dental College | Kansas City, Missouri | Inactive | |
| Delta Pi | 1924–1934 | University of California | Berkeley, California | Inactive | |
| 1924–1940 | Canisius College | Buffalo, New York | Inactive | |
| Kappa Rho | 1924–1929 | University of Maryland, Baltimore | Baltimore, Maryland | Inactive | |
| 1925–1934 | Missouri School of Mines and Metallurgy | Rolla, Missouri | Inactive | |
| 1925–1929 | Drury College | Springfield, Missouri | Inactive | |
| 1925–1925 | Clarkson University | Potsdam, New York | Inactive | |
| 1925–1940 | Creighton University | Omaha, Nebraska | Inactive | |
| 1927–1929 | Mississippi State University | Mississippi State, Mississippi | Inactive | |
| 1928–1929 | Miami University | Oxford, Ohio | Inactive | |
| Delta Phi | 1927–1930 | Lombard College | Galesburg, Illinois | Inactive | |
| c. 1927–1933 | Lincoln and Lee University | Kansas City, Missouri | Inactive | |
| Mu Mu | –1933 | Coe College | Cedar Rapids, Iowa | Inactive | |
| Pi Pi | 1928–1934 | University of Illinois | Urbana, Illinois | Inactive | |
| Alpha Eta | 1928–1931 | Ohio State University | Columbus, Ohio | Inactive | |
| 1928–1933 | University of Missouri, Kansas City | Kansas City, Missouri | Inactive | |
| 1928–1929 | University of Montana | Missoula, Montana | Inactive | |
| 1928–1929 | University of North Dakota | Grand Forks, North Dakota | Inactive | |
| 1928–1929 | Montana State University | Bozeman, Montana | Inactive | |
| 1928–1929 | University of Utah | Salt Lake City, Utah | Inactive | |
| 1928–1929 | Whitman College | Walla Walla, Washington | Inactive | |
| Alpha Mu | – 1936 | Southwestern, The College of the Mississippi Valley | Memphis, Tennessee | Inactive | |
| 1946–1946 | University of Southern California | Los Angeles, California | Inactive | |
| 1949 –1949 | Wichita State University | Wichita, Kansas | Inactive | |
| 1949–1949 | University of Arizona | Tucson, Arizona | Inactive | |
| Kappa Theta | 1997–xxxx ? | University of Pennsylvania | Philadelphia, Pennsylvania | Inactive | |