| Office | Name | Date |
| Prime Minister | William Ewart Gladstone | 1 February 188620 July 1886 |
| First Lord of the Treasury | William Ewart Gladstone | 1 February 188620 July 1886 |
| Leader of the House of Commons | William Ewart Gladstone | 1 February 188620 July 1886 |
| Chancellor of the Exchequer | Sir William Vernon Harcourt | 6 February 1886 |
| Parliamentary Secretary to the Treasury | Arnold Morley | 6 February 1886 |
| Financial Secretary to the Treasury | Henry Fowler | 6 February 1886 |
| Junior Lords of the Treasury | Sir Edward James Reed | 13 February 188620 July 1886 |
| Junior Lords of the Treasury | Cyril Flower | 13 February 188620 July 1886 |
| Junior Lords of the Treasury | George Leveson-Gower | 13 February 188620 July 1886 |
| Lord Chancellor | Sir Farrer Herschell | 6 February 1886 |
| Lord President of the Council | John Spencer, 5th Earl Spencer | 6 February 1886 |
| Lord Privy Seal | William Ewart Gladstone | 17 February 1886 |
| Secretary of State for the Home Department | Hugh Childers | 6 February 1886 |
| Under-Secretary of State for the Home Department | Henry Broadhurst | 6 February 1886 |
| Secretary of State for Foreign Affairs | Archibald Primrose, 5th Earl of Rosebery | 6 February 1886 |
| Parliamentary Under-Secretary of State for Foreign Affairs | James Bryce | 7 February 1886 |
| Secretary of State for War | Henry Campbell-Bannerman | 6 February 1886 |
| Under-Secretary of State for War | William Mansfield, 1st Viscount Sandhurst | 6 February 1886 |
| Financial Secretary to the War Office | Herbert Gladstone | 6 February 1886 |
| Surveyor-General of the Ordnance | William Woodall | 6 February 1886 |
Secretary of State for the Colonies and Leader of the House of Lords | Granville Leveson-Gower, 2nd Earl Granville | 6 February 1886 |
| Under-Secretary of State for the Colonies | George Osborne Morgan | 6 February 1886 |
| Secretary of State for India | John Wodehouse, 1st Earl of Kimberley | 6 February 1886 |
| Under-Secretary of State for India | Sir Ughtred Kay-Shuttleworth | 7 February 1886 |
| Under-Secretary of State for India | Stafford Howard | 12 April 1886 |
| First Lord of the Admiralty | George Robinson, 1st Marquess of Ripon | 9 February 1886 |
| Parliamentary and Financial Secretary to the Admiralty | John Tomlinson Hibbert | 9 February 1886 |
| Civil Lord of the Admiralty | Robert Duff | 15 February 1886 |
| Chief Secretary for Ireland | John Morley | 6 February 1886 |
| Lord Lieutenant of Ireland | John Hamilton-Gordon, 1st Marquess of Aberdeen and Temair | 8 February 1886 |
| President of the Local Government Board | Joseph Chamberlain | 6 February 1886 |
| President of the Local Government Board | James Stansfeld | 3 April 1886 |
| Parliamentary Secretary to the Local Government Board | Jesse Collings | 6 February 1886 |
| Parliamentary Secretary to the Local Government Board | William Copeland Borlase | 3 April 1886 |
| Secretary for Scotland | George Trevelyan, 2nd Baronet|George Trevelyan] | 8 February 1886 |
| Secretary for Scotland | John Ramsay, 13th Earl of Dalhousie | 5 April 1886 |
| President of the Board of Trade | A. J. Mundella | 17 February 1886 |
| Parliamentary Secretary to the Board of Trade | Charles Dyke Acland, 12th Baronet|Charles Dyke Acland] | 6 February 1886 |
| Vice-President of the Committee on Education | Sir Lyon Playfair | 13 February 1886 |
| Chancellor of the Duchy of Lancaster | Edward Heneage | 6 February 1886 |
| Chancellor of the Duchy of Lancaster | Sir Ughtred Kay-Shuttleworth | 16 April 1886 |
| Paymaster General | Thomas Hovell-Thurlow-Cumming-Bruce, 5th Baron Thurlow | 3 April 1886 |
| Postmaster-General | George Glyn, 2nd Baron Wolverton | 17 February 1886 |
| First Commissioner of Works | Albert Parker, 3rd Earl of Morley | 17 February 1886 |
| First Commissioner of Works | Victor Bruce, 9th Earl of Elgin | 16 April 1886 |
| Attorney General | Sir Charles Russell | 9 February 1886 |
| Solicitor General | Sir Horace Davey | 16 February 1886 |
| Judge Advocate General | John William Mellor | 22 February 1886 |
| Lord Advocate | John Balfour | 13 February 1886 |
| Solicitor General for Scotland | Alexander Asher | 13 February 1886 |
| Attorney General for Ireland | Samuel Walker, 1st Baronet|Samuel Walker] | February 1886 |
| Solicitor General for Ireland | Hugh Hyacinth O'Rorke MacDermot | February 1886 |
| Lord Steward of the Household | John Townshend, 1st Earl Sydney | 10 February 1886 |
| Lord Chamberlain of the Household | Valentine Browne, 4th Earl of Kenmare | 10 February 1886 |
| Vice-Chamberlain of the Household | Frederick Lambert, Viscount Kilcoursie | 19 February 1886 |
| Master of the Horse | Richard Boyle, 9th Earl of Cork | 10 February 1886 |
| Treasurer of the Household | Victor Bruce, 9th Earl of Elgin | 17 February 1886 |
| Comptroller of the Household | Edward Marjoribanks | 10 February 1886 |
| Captain of the Gentlemen-at-Arms | Charles Hanbury-Tracy, 4th Baron Sudeley | 10 February 1886 |
| Captain of the Yeomen of the Guard | William Monson, 7th Baron Monson | 10 February 1886 |
| Master of the Buckhounds | Charles Harbord, 5th Baron Suffield | 17 February 1886 |
| Mistress of the Robes | vacant | — |
| Lords in Waiting | Frederick Methuen, 2nd Baron Methuen | 16 February 188620 July 1886 |
| Lords in Waiting | Thomas Hovell-Thurlow-Cumming-Bruce, 5th Baron Thurlow | 16 February 188620 July 1886 |
| Lords in Waiting | Francis Stonor, 4th Baron Camoys | 16 February 188620 July 1886 |
| Lords in Waiting | Robert Offley Ashburton Milnes, 2nd Baron Houghton | 16 February 188620 July 1886 |
| Lords in Waiting | William Edwardes, 4th Baron Kensington | 1 March 188620 July 1886 |
| Lords in Waiting | Henry Tufton, 1st Baron Hothfield | 1 March 188620 July 1886 |
| Lords in Waiting | Thomas Lister, 4th Baron Ribblesdale | 1 March 188627 March 1886 |
| Extra Lord in Waiting | Mortimer Sackville-West, 1st Baron Sackville | 1 October 18761 October 1888 |