Holzinger is known for his research on the use of factor analysis to study human intelligence. He developed the theory that human intelligence consists of three types of abilities, or factors. This theory has since become the basis of many contemporary hierarchical theories of intelligence. He conducted much of his research as a member of the Unitary Traits Committee, which he and Edward Thorndike had established in 1931. He also collaborated with Horatio Newman and Frank N. Freeman on an early, large-scale twin study that began in 1927. According to M. Susan Lindee, this study "...was extraordinary in its depth and complexity".