Hot Start Polymerase Chain Reaction (PCR)
Hot Start Polymerase Chain Reaction (PCR) is a scholarly work by Joseph Sambrook, published in 2018 in ''Cold Spring Harbor Protocols''. The main subjects of the publication include polymerase chain reaction, real-time polymerase chain reaction, inverse polymerase chain reaction, In silico PCR, CRISPR, primer dimer, hot start PCR, polymerase, genetically modified animal, chemistry, Multiple displacement amplification, DNA polymerase, primer, deoxyribonucleic acid, molecular biology, and Polymerase chain reaction optimization. The purpose of hot start polymerase chain reaction (PCR) is to optimize the yield of the desired amplified product in PCRs and, simultaneously, to suppress nonspecific amplification and formation of primer dimers.