Holtfreter's solution


Holtfreter's solution is a balanced salt solution that was developed by the developmental biologist Johannes Holtfreter for studying amphibian embryos and to reduce bacterial infections. As a specialised aqueous solution, it finds use in aquaria to prevent infections for early stage amphibians, where it is typically mixed with soft tap water. Amphibians such as axolotls prefer a hard water solution.

Composition

Molarity of the component salts are as follows:
NaCl0.059 M
KCl0.00067 M
CaCl20.00076 M
NaHCO30.0024 M