High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy
High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy is a scholarly work by Jim Swoger and Ernst H K Stelzer, published in 2007 in ''Nature Methods''. The main subjects of the publication include fluorescence microscope, materials science, Optical sectioning, Confocal, fluorescence, image resolution, optics, biological specimen, scanning confocal electron microscopy, Light sheet fluorescence microscopy, photochemotherapy, Bioimage informatics, optical microscope, microscopy, bright-field microscopy, resolution, photoactivated localization microscopy, Fluorescence-lifetime imaging microscopy, super-resolution microscopy, confocal microscopy, microscope, and deconvolution. The authors report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples.