Founded as AC Vozovka in 1935, the club played its matches next to the railway line from Trnava to Leopoldov. In 1950 the club would be renamed to Lokomotíva Trnava. In 1999, long-time player Peter Moravčík took over the club.
Youth
Lokomotiva Trnava is known for its education of young footballers. In 2024, the under-13 team won the championship title in the second league west, while the under-15 category finished second in its league competition. The majority of youth players that show promise in the Lokomotiva academy are transferred to Spartak Trnava.
Stadium
In addition to a football field with an artificial surface, two grass footballfields and asphalt fields, the sports complex also includes tennis courts and mini golf courses. The football fields are used for the activities of FK Lokomotíva Trnava. The first football field with artificial turf in Trnava was ceremonially opened at Lokomotiv Trnava. In 2025, the complex saw major re-construction funded by the Trnava city.