Dental pulp test
Dental pulpal testing is a clinical and diagnostic aid used in dentistry to help establish the health of the dental pulp within the pulp chamber and root canals of a tooth. Such investigations are important in aiding dentists in devising a treatment plan for the tooth being tested.
There are two major types of dental pulp tests. Vitality testing assesses the blood supply to the tooth, whilst sensitivity testing tests the sensory supply.
Clinical application
Dental pulp tests are valuable techniques used to establish the pulpal health status of a tooth in dentistry. The diagnostic information obtained from pulpal testing is then used alongside a patient's history, clinical and radiographic findings to determine a diagnosis and prognosis of the tooth.Pulp tests are useful for the following procedures in dentistry:
- diagnosis of endodontic pathology,
- localisation of tooth pain,
- differentiating between odontogenic and non-odontogenic pain,
- assessing pulpal status following dental trauma,
- establishment of pulpal health prior to prosthodontic treatment.
Sensitivity testing
Sensitivity tests assess the sensory response of a tooth to an external stimulus, results which may be extrapolated to indirectly determine pulpal health status. Sensory stimuli, such as heat, cold or an electrical current, are applied to the tooth in question in order to stimulate the nocireceptors within the pulp. The type of sensory fibres activated and therefore the response felt by the patient depends on the stimulus used. Sensibility testing is based on Brännström's hydrodynamic theory, which postulates that the activation of nocireceptors is caused by fluid movement within the dentinal tubules in response to thermal, electrical, mechanical or osmotic stimuli.Responses to sensitivity testing
There are three primary outcomes of a pulp sensitivity test:- A normal response — healthy pulps respond to sensitivity testing by eliciting a short, sharp pain which subsides when the stimulus is removed, indicating that the nerve fibres are present and responsive.
- A heightened or prolonged response — an exaggerated or lingering response to sensitivity testing indicates some degree of pulpal inflammation. If the pain is pronounced yet subsides once the stimulus has been removed, a diagnosis of reversible pulpitis may be probable. However, a lingering pain which continues despite the removal of the stimulus is indicative of irreversible pulpitis.
- No response — lack of response to sensitivity testing suggests that the nerve supply to the tooth has been diminished, as in the case of pulpal necrosis or in previously root treated canals.
Types of sensitivity tests
Thermal tests
Thermal testing, which involves the application of either hot or cold stimuli to the tooth, is the most common form of sensibility test.A number of products are available for cold testing, each with varying melting points. Although household ice is cheap and easy to obtain, it is not as accurate as colder products. Dry ice can be used, however there have been concerns regarding the damaging effects of using something so cold in the oral cavity despite evidence to suggest that dry ice has no negative impact on mucosal or tooth structure. Refrigerant sprays, such as ethyl chloride, 1,1,1,2-tetrafluoroethane or a propane/butane/isobutane gas mixture are further commonly used cold tests. Cold testing is thought to stimulate Type Aδ fibres in the pulpal tissue, which elicit a short, sharp pain.
Heat tests include using heated instruments, such as a ball-ended probe or gutta-percha, a rubber commonly used in root canal procedures. Such tests are less commonly used as they are thought to be less accurate than cold tests, and may be more likely to cause damage to the teeth and surrounding mucosa.
Electric pulp testing
An electrical current can be applied to the tooth in order to generate an action potential in the Type Aδ fibres within pulp, eliciting a neurological response. Such tests are conducted by applying a conducting medium on a dried tooth and placing the probe tip of an electric pulp tester on the surface of the tooth closest to the pulp horn. The patient is then directed to hold the end of the conducting probe to complete the circuit and asked to let go of the probe when a 'tingling' sensation is felt.The use of electric pulp testing has been questioned in patients with traditional cardiac pacemakers despite no evidence of interferences in humans, particularly with more modern devices. Care must be taken if using an electric pulp test on a tooth adjacent to metallic restorations, as these can create electrical conduction and yield false negative results.
Anaesthesia testing
When pulp testing results are inconclusive and that patients cannot localise or specify the pain or symptoms, an anaesthetic would be helpful and be used. The most posterior tooth in the area where the pain resonates undergoes anaesthesia by either infiltration or intraligamentary injection until pain diminishes. If the pain is still present, the procedure is repeated on the mesial teeth, one by one until the pain diminishes and is gone. If one can still not determine the source of the pain, the procedure will be repeated on the opposite arch. In the case that the pain cannot be localised to either the maxillary or mandibular arch, an inferior alveolar nerve block would be used. If the pain stops, such would imply that it involves teeth of the mandibular arch.Test cavity
The test cavity technique is only used as a last resort when results produced by all other methods above are inconclusive. High-speed burs are used without anaesthetic, drilling through enamel, or restorations to dentine. Throughout the drilling process, the patient is asked whether a painful sensation is felt, which would indicate pulpal vitality. In the event of a vital pulp, a painful response is provoked when dentin is contacted by the bur and the procedure will be stopped. A restoration would be then placed. Contrarily, when compared with vital pulp, pulp with partial necrosis will not be stimulated as extensively. In the case of partial necrosis, access to and into dentine would be needed, with the dentist progressively invading and drilling deeper into dentine, checking the sensory response—which is usually without sensory response because of the partial necrosis. Due to the invasiveness and possible anxiety that it may generate in patients, the test cavity technique is generally avoided. Also, there is little literature supporting its effectiveness, and it has been relatively anecdotal within clinical practice.Limitations of sensitivity testing
All tests have some limitations and test results should be interpreted by an experienced dentist under the bidirectional consideration of both clinical symptoms and radiography. Sensitivity tests only indicate the presence or absence of the nerve supply to an individual tooth. Even though a prolonged response to aforementioned tests indicate pulpal inflammation, the degree of inflammation or innervation cannot be inferred from these tests.False positive or false negative results are possible when performing a sensitivity testing. A false positive response occurs when a patient is respondent to sensitivity testing despite a lack of sensory tissue in the tooth that is being tested. Such responses may occur due to innervation of adjacent teeth due to inadequate isolation of the tooth being tested, or in anxious patients who perceive pain despite no sensory stimulus, or in multi-rooted teeth which still have residual pulpal tissue residing in canals. False negative results occurs when innervated teeth do not respond to sensibility testing. Such can occur in individuals who have recently traumatised teeth, teeth with incomplete root development, teeth with heavy restorations or teeth that have significantly reduced pulp size due to production of tertiary or sclerotic dentine.
Pulpal sensitivity testing may be regarded as inferior to vitality testing as they do not definitively prove that the tooth has a blood supply and is vital. Nonetheless, electric pulp testing and cold testing tests have been found to be accurate and reliable in the case of assessing pulpal health, especially when tests are used in combination. In addition, cold testing is also more accurate than electric pulp in the case of running tests upon immature or traumatised teeth.
Despite the insights gained from sensitivity testing, a research study found that the density of nerve fibers and blood vessels in the pulp tissue, and the degree of oxygen saturation, may play a crucial role in interpreting the results. The presence of a higher density of nerve fibers may contribute to a lower threshold for electrical stimulation, suggesting the involvement of neural factors in pulp sensibility. Moreover, the positive correlation between blood vessel density and oxygen saturation, as well as the negative correlation between nerve fiber density and electrical voltage perception, provide valuable insights into the complex nature of dental pulp. Therefore, in addition to the standard sensitivity testing, more objective and accurate methods such as pulse oximetry might be necessary for a comprehensive understanding of pulp vitality. However, the findings of this study should be generalized with caution due to its small sample size and focus on healthy teeth extracted for orthodontic reasons.