Direct polymerase chain reaction from whole blood without DNA isolation
Direct polymerase chain reaction from whole blood without DNA isolation is a scholarly work, published in 2000 in ''Annals of Clinical Biochemistry''. The main subjects of the publication include whole blood, deoxyribonucleic acid extraction, polymerase chain reaction, chemical reagent, real-time polymerase chain reaction, inverse polymerase chain reaction, chromatography, biology, primer dimer, hot start PCR, gene, chemistry, primer, Bloodstain, multiplex polymerase chain reaction, deoxyribonucleic acid, molecular biology, and Polymerase chain reaction optimization. The authors have developed a novel reagent cocktail that effectively suppresses these inhibitory substances and makes DNA isolation from blood unnecessary for PCR.