Detection of Protein–Protein Interactions Using Tandem Affinity Purification
Detection of Protein–Protein Interactions Using Tandem Affinity Purification is a scholarly work by Ian Goodfellow and Dalan Bailey, published in 2014 in ''Methods in Molecular Biology''. The main subjects of the publication include tandem, mass spectrometry, fusion protein, peptide, tandem mass spectrometry, Tobacco etch virus, monoclonal antibody therapy, Target protein, protein expression (biotechnology), affinity chromatography, FLAG-tag, biochemistry, protein purification, chromatography, peptidase, protein tag, streptavidin, chemistry, computational biology, elution, protein-protein interaction, and Tandem affinity purification. Tandem affinity purification (TAP) is an invaluable technique for identifying interaction partners for an affinity tagged bait protein.