Defunct AF2 teams
The following is a list of teams that, at one time, played in the AF2. This does not include teams in AF2 when the Board of Directors formed the New AFL in 2010.
| Team | City | Arena | Founded | First AF2 season | Final AF2 season | Notes |
| Alabama Steeldogs | Birmingham, Alabama | Birmingham Jefferson Convention Center | 1999 | 2000 | 2007 | - |
| Albany Conquest | Albany, New York | Times Union Center | 2002 | 2002 | 2008 | Same franchise as Albany Firebirds |
| Albany Firebirds | Albany, New York | Times Union Center | 2002 | 2009 | 2009 | Same franchise as Albany Conquest |
| Amarillo Dusters | Amarillo, Texas | Amarillo Civic Center | 2003 | 2005 | 2009 | - |
| Arkansas Twisters | North Little Rock, Arkansas | Verizon Arena | 1999 | 2000 | 2009 | - |
| Augusta Stallions | Augusta, Georgia | James Brown Arena | 1999 | 2000 | 2002 | - |
| Austin Wranglers | Austin, Texas | Frank Erwin Center | 2003 | 2008 | 2008 | - |
| Bakersfield Blitz 2002–2003 | Bakersfield, California | Rabobank Arena | 2002 | 2002 | 2003 | Same franchise as Central Valley Coyotes |
| Bakersfield Blitz 2004–2007 | Bakersfield, California | Rabobank Arena | 2004 | 2004 | 2007 | - |
| Baton Rouge Blaze | Baton Rouge, Louisiana | Baton Rouge River Center Arena | 2000 | 2001 | 2001 | - |
| Bismarck Bandits | Bismarck, North Dakota | Bismarck Event Center | 2001 | Never played | - | |
| Boise Burn | Boise, Idaho | Qwest Arena | 2006 | 2007 | 2009 | - |
| Bossier–Shreveport Battle Wings | Bossier City, Louisiana | CenturyTel Center | 2001 | 2001 | 2009 | - |
| Cape Fear Wildcats | Fayetteville, North Carolina | Crown Coliseum | 2002 | 2002 | 2004 | Same franchise as South Georgia Wildcats |
| Carolina Rhinos | Greenville, SC | Bi-Lo Center | 1999 | 2000 | 2002 | - |
| Central Valley Coyotes | Fresno, California | Selland Arena | 2002 | 2004 | 2009 | Same franchise as Bakersfield Blitz 2002–2003 |
| Charleston Swamp Foxes | North Charleston, SC | North Charleston Coliseum | 1999 | 2000 | 2003 | - |
| Cincinnati Jungle Kats | Cincinnati, Ohio | U.S. Bank Arena | 2006 | 2007 | 2007 | - |
| Cincinnati Swarm | Cincinnati, Ohio | U.S. Bank Arena | 2002 | 2003 | 2003 | - |
| Columbus Wardogs | Columbus, Georgia | Columbus Civic Center | 2000 | 2001 | 2004 | Same franchise as Mississippi Headhunters |
| Corpus Christi Sharks | Corpus Christi, Texas | American Bank Center | 2006 | 2007 | 2009 | - |
| Daytona Beach ThunderBirds | Daytona Beach, Florida | Ocean Center | 2005 | 2008 | 2008 | - |
| Everett Hawks | Everett, WA | Everett Events Center | 2001 | 2006 | 2007 | - |
| Florida Firecats | Estero, Florida | Germain Arena | 2001 | 2001 | 2009 | - |
| Fort Wayne Fusion | Fort Wayne, Indiana | Allen County War Memorial Coliseum | 2006 | 2007 | 2007 | - |
| Fresno Frenzy | Fresno, California | Selland Arena | 2001 | 2002 | 2002 | - |
| Green Bay Blizzard | Ashwaubenon, Wisconsin | Resch Center | 2003 | 2003 | 2009 | - |
| Greensboro Prowlers | Greensboro, NC | Greensboro Coliseum | 1999 | 2000 | 2003 | - |
| Hawaiian Islanders | Honolulu, Hawaii | Neal S. Blaisdell Center | 2001 | 2002 | 2004 | - |
| Iowa Barnstormers | Des Moines, Iowa | Wells Fargo Arena | 1995 | 2001 | 2009 | - |
| Jacksonville Tomcats | Jacksonville, Florida | Jacksonville Memorial Coliseum | 1999 | 2000 | 2002 | - |
| Kentucky Horsemen | Lexington, Kentucky | Rupp Arena | 2002 | 2008 | 2009 | - |
| Lafayette Roughnecks | Lafayette, Louisiana | CajunDome | 2000 | 2001 | 2001 | - |
| Laredo Law | Laredo, Texas | Laredo Entertainment Center | 2003 | 2004 | 2004 | - |
| Laredo Lobos | Laredo, Texas | Laredo Entertainment Center | 2005 | 2007 | 2007 | - |
| Lincoln Lightning | Lincoln, Nebraska | Pershing Municipal Auditorium | 1998 | 2001 | 2001 | - |
| Louisville Fire | Louisville, Kentucky | Freedom Hall | 2000 | 2001 | 2008 | - |
| Lubbock Renegades | Lubbock, Texas | City Bank Coliseum | 2006 | 2007 | 2008 | - |
| Macon Knights | Macon, Georgia | Macon Coliseum | 2000 | 2001 | 2006 | - |
| Mahoning Valley Thunder | Youngstown, Ohio | Covelli Centre | 2007 | 2007 | 2009 | - |
| Manchester Wolves | Manchester, New Hampshire | Verizon Wireless Arena | 2002 | 2004 | 2009 | Same franchise as Mohegan Wolves |
| Memphis Xplorers | Southaven, Mississippi | DeSoto Civic Center | 2000 | 2001 | 2006 | - |
| Milwaukee Iron | Milwaukee, Wisconsin | Bradley Center | 2009 | 2009 | 2009 | - |
| Mississippi Headhunters | Biloxi, Mississippi | 2001 | Never played | Same franchise as Columbus Wardogs | ||
| Mobile Wizards | Mobile, Alabama | Mobile Civic Center | 2001 | 2002 | 2002 | - |
| Mohegan Wolves | Uncasville, Connecticut | Mohegan Sun Arena | 2002 | 2002 | 2003 | Same franchise as Manchester Wolves |
| New Haven Ninjas | New Haven, Connecticut | New Haven Coliseum | 2001 | 2002 | 2002 | - |
| Norfolk Nighthawks | Norfolk, Virginia | Norfolk Scope | 1999 | 2000 | 2003 | - |
| Oklahoma City Yard Dawgz | Oklahoma City, Oklahoma | Cox Convention Center | 2004 | 2004 | 2009 | - |
| Pensacola Barracudas | Pensacola, Florida | Pensacola Civic Center | 1999 | 2000 | 2002 | - |
| Peoria Pirates | Peoria, Illinois | Peoria Civic Center | 1999 | 2001 | 2009 | - |
| Quad City Steamwheelers | Moline, Illinois | iWireless Center | 2000 | 2000 | 2009 | - |
| Richmond Speed | Richmond, Virginia | Richmond Coliseum | 1999 | 2000 | 2003 | - |
| Rio Grande Valley Dorados | Hidalgo, Texas | State Farm Arena | 2004 | 2004 | 2009 | - |
| Roanoke Steam | Roanoke, Virginia | Roanoke Civic Center | 1999 | 2000 | 2002 | - |
| Rochester Brigade | Rochester, New York | Blue Cross Arena | 2000 | 2001 | 2003 | - |
| San Diego Riptide | San Diego, California | San Diego Sports Arena | 2001 | 2002 | 2005 | - |
| South Georgia Wildcats | Albany, Georgia | Albany Civic Center | 2002 | 2005 | 2009 | Same franchise as Cape Fear Wildcats |
| Spokane Shock | Spokane, Washington | Spokane Veterans Memorial Arena | 2005 | 2006 | 2009 | - |
| Stockton Lightning | Stockton, California | Stockton Arena | 2006 | 2006 | 2009 | - |
| Tallahassee Thunder | Tallahassee, Florida | Tallahassee-Leon County Civic Center | 1999 | 2000 | 2002 | - |
| Tennessee Valley Vipers | Huntsville, Alabama | Von Braun Center | 2000 | 2000 | 2009 | - |
| Texas Copperheads | Cypress, Texas | Richard E. Berry Educational Support Center | 2005 | 2007 | 2008 | - |
| Toledo Bullfrogs | Toledo, Ohio | Lucas County Arena | 2008 | Never played | - | |
| Tri-Cities Fever | Kennewick, Washington | Toyota Center | 2004 | 2007 | 2009 | - |
| Tulsa Talons | Tulsa, Oklahoma | BOK Center | 1999 | 2000 | 2009 | - |
| Wichita Stealth | Wichita, Kansas | Kansas Coliseum | 1999 | 2001 | 2004 | - |
| Wilkes-Barre/Scranton Pioneers | Wilkes-Barre, Pennsylvania | Mohegan Sun Arena at Casey Plaza | 2001 | 2002 | 2009 | - |