BAF agar
BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi. It was first described by in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981. The acidic pH of BAF agar inhibits bacterial growth.
Typical composition
BAF agar typically contains:- 30.0 g/L glucose
- 2.0 g/L peptone
- 0.2 g/L yeast extract
- 0.5 g/L KH2PO4
- 0.5 g/L MgSO4.7 H2O
- 10.0 mg/L FeCl3.6 H2O
- 1.0 mg/L ZnSO4.7 H2O
- 5.0 mg/L MnSO4
- 100.0 mg/L CaCl2.2 H2O
- 50.0 μg/L thiamine HCl
- 1.0 μg/L biotin
- 100.0 μg/L folic acid
- 50.0 μg/L inositol
- 15 g/L agar