André Sentenac


Georges André Sentenac is a French molecular biologist specializing in gene transcription.
Married to Pierrette Balse, professor of mathematics at the University of Orsay, they had three children, all scientists, Anne, Marion and Daniel.

Biography

He was elected correspondent of the French Academy of sciences in 1999 and then a member of the Institute in 2007.

Scientific career

During his postdoctoral internship in Dr. George Acs' laboratory in New York City, Sentenac addresses the regulation of gene expression by showing that appropriate hormonal treatment forces a young male cockerel to produce certain specific components of the egg. Back in France, to bypass the complexity of higher organisms, he chose to work on the yeast Saccharomyces cerevisiae. In the following years, this yeast will become an essential study model in the molecular genetics of eukaryotic cells.
First, he isolated and identified the essential components of the molecular machinery responsible for gene transcription in yeast. He gave the first complete description of the three forms of nuclear RNA polymerases which are composed of the assembly of many subunits with various functions. Focusing his efforts on the transcription of the large family of class III genes encoding tRNAs, 5S RNA and other small RNAs, he isolated the general transcription factors TFIIIC and TFIIIB and described the cascade of protein-DNA and protein-protein interaction leading to the recruitment of RNA polymerase III on DNA and the initiation of transcription. A total of 26 proteins are dedicated to the transcription of class III genes. In particular, it shows that the TFIIIC factor consists of two protein modules capable of binding to two distinct promoter elements spaced differently according to genes, of lifting chromatin repression and of recruiting TFIIIB.

Awards and honours

More representative publications

  • Sentenac, A. Eukaryotic RNA polymerases. CRC Crit. Rev. Biochem. 18: 31-90
  • Marzouki, N., CaMier, S., Ruet, A, Moenne, A, and  Sentenac, A. Selective proteolysis defines two DNA binding domains in yeast transcription factor τ. Nature 323: 176-178
  • Margottin, F., Dujardin, G., Gérard, M., Égly, J.-M., Huet, J. and Sentenac, A.  Participation of the TATA factor in transcription of the yeast U6 gene by RNA polymerase C. Science 251: 424-426
  • Burnol, A-F., Margottin, F., Huet, J., Almouzni, G., Prioleau, M.-N., Méchali, M., and Sentenac, A. TFIIIC relieves repression of U6 snRNA transcription by chromatin. Nature 62: 475-477
  • Marsolier, M.-C., Tanaka, S., Livingstone-Zatchej, M., Grunstein, M., Thoma, F. and Sentenac, A. Reciprocal interferences between nucleosomal organization and transcriptional activity of the yeast SNR6 gene. Genes & Dev. 9: 410-422
  • Rüth, J., Conesa, C., Dieci, G., Lefebvre, O., Düsterhöft, A, Ottonello,  S., and Sentenac, A. A suppressor of mutations in the class III transcription system encodes a component of yeast TFIIIB. EMBO J. 15: 1941-1949
  • Dieci, G. and Sentenac, A. Facilitated recycling pathway for RNA polymerase III. Cell 84: 245-252
  • Chédin, S., Ferri, M.L., Peyroche, G., Andrau, J.C., Jourdain, S., Lefebvre, O., Werner, M., CarLes, C., and Sentenac, A. The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation. Cold Spring Harb. Symp. Quant. Biol. 63: 381-389
  • Oficjalska-Pham, D., Harismendy, O., Smagowicz, W.J., Gonzalez de Peredo, A, Boguta, M., Sentenac, A. and Lefebvre, O. General repression of RNA polymerase III transcription is triggered by protein phosphatase type 2A-mediated dephosphorylation of Maf1 Mol. Cell 22: 623-632.
  • Ducrot, C., Lefebvre, O., Landrieux, E., Guirouilh-Barbat, J., Sentenac, A. and Acker, J. Reconstitution of the yeast RNA polymerase III transcription system with all recombinant factors. J. Biol. Chem. 281: 11685-11692
  • Sentenac, A. and Riva, M. Odd RNA polymerases or the AC of eukaryotic transcription. Biochim. Biophys. Acta 1829 : 251-257

Main books