2025 Knoxville Challenger

The 2025 Knoxville Challenger was a professional tennis tournament played on indoor hard courts. It was the 21st edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Knoxville, United States between 3 and 9 November 2025.

Singles main-draw entrants

¹ Rankings are as of October 27, 2025.

The following players received wildcards into the singles main draw:

The following player received entry into the singles main draw through the Junior Accelerator programme:

The following player received entry into the singles main draw as an alternate:

The following players received entry from the qualifying draw:

The following player received entry as a lucky loser:

Mitchell Krueger def. Darwin Blanch 6–7, 6–4, 6–1.

Patrick Harper / Quinn Vandecasteele def. Mitchell Krueger / Jody Maginley 6–7, 7–6, .