2025 Cincinnati Open – Women's singles
Iga Świątek defeated Jasmine Paolini in the final, 7–5, 6–4 to win the women's singles tennis title at the 2025 Cincinnati Open. It was her first Cincinnati Open title and eleventh WTA 1000 title. Świątek did not lose a set during the tournament.
Aryna Sabalenka was the defending champion, but lost in the quarterfinals to Elena Rybakina.
Seeds
All seeds received a bye into the second round.In addition to the seeded players, the following players received performance byes into the second round after reaching the final in Montreal. Both players later withdrew from the tournament and were replaced by lucky losers.
Draw
Finals
Seeded players
The following are the seeded players. Seedings are based on WTA rankings as of July 28, 2025. Rankings and points before are as of August 11, 2025.Unlike the ATP, the WTA is only dropping points from the 2024 Cincinnati Open this week, since points for the 2024 National Bank Open were already dropped from rankings as of August 11, 2025.
| Seed | Rank | Player | Points before | Points defending | Points earned | Points after | Status | ||||||||||||||
| 1 | 1 | Aryna Sabalenka | 12,010 | 1,000 | 215 | 11,225 | Quarterfinals lost to  Withdrawn seeded playersThe following players would have been seeded, but withdrew before the tournament began.
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