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BerlinThe 1964–65 season saw one new club in the league, Viktoria 89 Berlin, promoted from the Amateurliga Berlin, while no club had been relegated from the Bundesliga to the league. After a home-and-away round of 18 games each the league was split into a championship and a relegation round with five clubs each. Because Tennis Borussia Berlin failed in the promotion round and Spandauer SV declined SC Tasmania 1900 Berlin was directly promoted to the Bundesliga to replace Hertha BSC which had its licence revoked.
WestThe 1964–65 season saw three new clubs in the league, Eintracht Gelsenkirchen and Homberger SV, promoted from the Verbandsliga, while Preußen Münster had been relegated from the Bundesliga to the league.
Top goalscorersThe top goal scorers in the Regionalliga Wset:
| Rank | Player | Club | Goals | | 1 | 
South-WestThe 1964–65 season saw two new clubs in the league, Germania Metternich, promoted from the Amateurliga, while 1. FC Saarbrücken had been relegated from the Bundesliga to the league.
Top goalscorersThe top goal scorers in the Regionalliga Südwest:
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