Ydc2 protein domain


In molecular biology, the protein domain, Ydc2, is a Holliday junction resolvase from the fission yeast Schizosaccharomyces pombe that is involved in the maintenance of mitochondrial DNA.

Function

The Ydc2 domains are enzymes that resolve Holliday junctions into separate DNA duplexes by cleaving DNA after 5'-CT-3, and 5'-TT-3, sequences.

Properties

The junction resolving enzymes are very diverse, but have the following properties in common:
Essentially, they are highly specific.

Limiting factors

Furthermore, the cleavage efficiency is affected by:
  • strand type
  • nucleotide sequence at cleavage site

Structure

This protein domain forms a ribonuclease H fold consisting of two beta sheets and one alpha helix, arranged as a beta-alpha-beta motif. Each beta sheet has five strands, arranged in a 32145 order, with the second strand being antiparallel to the rest.