| Route | Local Name | Type | Northern/Western Terminus | Southern/Eastern Terminus | Communities | Comments |
| Snyder's Road, Waterloo Street, Huron Street, Wilmot-Easthope Road | | 110m South of Huron Road | RR 6 | New Hamburg, Baden | |
| Walker Road | | Highway 7 & 8 | Oxford-Waterloo Road | | Minor rural route |
| Huron Street, Peel Street, Bleams Road, Ottawa Street | Arterial road | RR 1 | RR 54, Keewatin Avenue | New Hamburg, Mannheim, Kitchener | Intersects the Conestoga Parkway twice. Ottawa Street proposed extension from Keewatin Avenue to RR 17 |
| Manser Road, William Hastings Line, Hutchison Road, Queen's Bush Road, Nafziger Road | | RR 86 | Highway 7 & 8 | Wellesley, Linwood | |
| Gingerich Road, Snyders Road, Highland Road, Queen Street, Benton Street, Frederick Street | Arterial road | RR 51 | RR 61 | Baden, Kitchener | |
| Hutchison Road, Perth Line | | RR 5 | Road 105 | Wellesley | |
| King Street, Weber Street, Shantz Hill Road, Fountain Street, Coronation Boulevard, Dundas Street | | RR 17 | RR 43 / Highway 8 | St. Jacobs, Waterloo, Kitchener, Cambridge | Formerly Highway 8 between Freeport Diversion and RR 43 |
| Wilmot-Easthope Road, Erb's Road, Erb Street, Caroline Street, Bridgeport Road | | Perth County Road 43 | RR 29 | St. Agatha, Waterloo, Bridgeport | |
| Herrgott Road | Arterial road | RR 86 | RR 15 | St. Clements, Wallenstein | |
| William Hastings Line | Rural route | Road 116 | RR 5 | Perth County boundary | |
| Gerber Road, Notre Dame Drive, Queen Street, Bridge Street, New Dundee Road | | RR 5 | RR 28 | Wellesley, St. Agatha, New Dundee, Doon | |
| Kressler Road, Weimar Line, Moser-Young Road | | RR 12 | RR 70 | Bamberg | |
| Lobsinger Line, King Street | Arterial road | RR 5 | Dixon Street/Montgomery Road | St. Clements, Heidelberg, Waterloo, Kitchener | |
| Kressler Road | | RR 17 | RR 70 | Heidelberg, Erbsville | |
| Fountain Street, Ebycrest Road, Sawmill Road, Northside Drive, Hawkesville Road, Ament Line | | RR 5 | Highway 8 | St. Jacobs, Conestogo, Bloomingdale, Breslau, Cambridge | |
| Floradale Road | | Florapine Road | RR 86 | Floradale | |
| Bloomingdale Road | | RR 52 | RR 17 | Bridgeport | |
| Arthur Street | | Floradale Road | RR 85 | Elmira, North Woolwich | |
| Northfield Drive | | Wellington County limits | RR 15 | Waterloo, Conestogo | |
| Katherine Street | | Woolwich-Pilkington Townline | RR 17 | Winterbourne, Zuber Corners | |
| Hespeler Road, Water Street, Ainslie Street | | Wellington County limits | RR 81 / Highway 24 | Glenchristie, Cambridge | Formerly Highway 24 |
| Maryhill Road | | Woolwich-Guelph Townline | RR 26 | Maryhill, Bloomingdale | |
| St. Charles Street | | RR 17 | RR 25 | Maryhill, Bloomingdale | |
| Clyde Road, Samuelson Street, Beverly Street, Wellington Street, Main Street, Queen's Square, North Square, South Square | | RR 42 | Village Road | Cambridge, Clyde | |
| Homer Watson Boulevard, Fountain Street | | RR 4 | RR 8 | Cambridge, Doon, Kitchener | Includes small section of Huron Road from on and off ramps to Homer Watson Boulevard. |
| Lancaster Street | | RR 52 | RR 55 | Kitchener, Bridgeport | |
| Shantz Station Road | | RR 26 | RR 31 | Kossuth | |
| Kossuth Road | | RR 17 | RR 24 | Kossuth | |
| Townline Road | | Wellington County Road 34 | Gore Road | Cambridge | |
| Franklin Boulevard | | Highway 401 | RR 81 | Cambridge | |
| Allen Street | | RR 9 | RR 15 | Uptown Waterloo | |
| Sportsworld Drive, Maple Grove Road | | RR 8 | RR 24 | Kitchener | |
| Eagle Street, Pinebush Road | Arterial road | RR 8 | RR 33 | Cambridge | |
| Bishop Street | | RR 8 | RR 36 | Cambridge | |
| Blair Road, George Street | | RR 28 | RR 75 | Cambridge | |
| Myers Road, Branchton Road | | Highway 24 | Brant County Road 144 | Cambridge, Littles Corners, Branchton | |
| Roseville Road | | Regional Road 58 | Cambridge city limits | Roseville, Orrs Lake | Shares roadway with Regional Road 71 between Dickie Settlement Road and Edworthy Sideroad |
| Dumfries Road | | Regional Road 46 | Regional Road 49 | Wrigley | |
| Wrigley Road, Scott Street, Main Street, Stanley Street | | Regional Road 58 | Regional Road 75 | Wrigley, Ayr | |
| Northfield Drive, Westmount Road | | Regional Road 15 | Regional Road 58 | Kitchener, Waterloo | |
| Foundry Street, Wilmot Centre Road | | Regional Road 1 | Regional Road 4 | Baden, Wilmot Centre | |
| Bridge Street | | RR 22 | Regional Road 17 | Waterloo, Bridgeport | |
| Courtland Avenue, Fairway Road | | Regional Road 6 | RR 17 | Kitchener | |
| Lackner Boulevard | | Highway 7 | RR 53 | Kitchener | |
| Victoria Street | | RR 70 | Highway 85, RR 17 | Kitchener | Continues past Highway 85 as both Highway 7 and RR 55 |
| Bleams Road, River Road | | RR 70, RR 8 | Wilson Avenue, RR 53 | Kitchener | Bleams Road extension from RR 69 to Wilson Avenue opened August 2025.. To be connected from RR 8 to Wabanaki Drive and Goodrich Drive as part of RR56 completion. |
| University Avenue | Arterial road | RR 70 | RR 52 | Waterloo | Name derives from the universities of Waterloo and Wilfrid Laurier, which it serves. |
| Bearinger Road, Fischer-Hallman Road, Roseville Road, Northumberland Street, Stanley Street, Swan Street | | RR 50 | Brant-Waterloo Road | Waterloo, Kitchener, Roseville, Ayr | |
| Bruce Street | | RR 55 | RR 6 | Kitchener | |
| Edna Street | | RR 55 | RR 6 | Kitchener | |
| Duke Street | | RR 68 | RR 6 | Kitchener | |
| Charles Street | | RR 55 | RR 4 | Kitchener | |
| Borden Avenue | | Unnamed laneway connecting Borden Avenue and Grenville Avenue | RR 64 | Kitchener | |
| Hayward Avenue | | 230m West of RR 53 | RR 53 | Kitchener | |
| Francis Street | | RR 63 | RR 15 | Kitchener | |
| Manitou Drive | | RR 53 | RR 28 | Kitchener | |
| Erbsville Road, Ira Needles Boulevard, Trussler Road | | RR 14 & 16 | Brant Waterloo Road | Erbsville, Waterloo, Kitchener, Mannheim, Plumtree | |
| Dickie Settlement Road, Roseville Road, Edworthy Sideroad | Rural route | Regional Road 28 | Regional Road 97 | North Dumfries | |
| Spragues Road, St. Andrews Street | | Regional Road 76 | Brant-Waterloo Road | Cambridge | Formerly Highway 24A |
| Grand Avenue | | RR 75 | RR 97 | Cambridge | |
| Park Hill Road | | RR 42 | RR 24 | Cambridge | |
| Can-Amera Parkway | | Highway 24 | RR 33 | Cambridge | |
| McQueen Shaver Boulevard | | Highway 24 | RR 36 | Cambridge, North Dumfries | South boundary road, to be extended to RR 33 |
| Listowel Road, Arthur Street South | Rural route | RR 86 | RR 15 / Highway 85 | Elmira | Formerly Highway 85 |
| Church Street, Line 86 | | Red Woods Drive | Sideroad 16 | Macton, Wallenstein, Elmira, Zuber Corners, Weissenburg | Formerly Highway 86 |
| Cedar Creek Road, Cedar Street, Concession Street, Main Street, Old Beverly Road | | RR 70 | Hamilton city limits | Cambridge, North Dumfries | Formerly Highway 97 |
| Route | Type | Local Name | Western/Southern Terminus | Eastern/Northern Terminus | Communities | Comments | - | - | - | - | - | - | - |
| Route | Type | Local Name | Western/Southern Terminus | Eastern/Northern Terminus | Communities | Comments | | Partial controlled access highway | Conestoga Parkway, Waterloo Regional Road 55 | Waterloo Regional Road 1 | Woolwich Guelph Townline Road | New Hamburg, Kitchener, Breslau | Part of the Conestoga Parkway. Shares the route with Highway 8, becoming Highway 7/8, from Kitchener to Stratford. |
| Partial controlled access highway | Conestoga Parkway, | Highway 401 | Waterloo Regional Road 1 | Cambridge, Kitchener, New Hamburg | Part of the Conestoga Parkway. Shares the route with Highway 7, becoming Highway 7/8, from Kitchener to Stratford. | - | - | - | - | - | - | - |
| Arterial road | Brantford Highway | Lockie Road | Waterloo Regional Road 24 | Cambridge | Becomes Waterloo Regional Road 24 near Cambridge city limits. | - | - | - | - | - | - | - |
| Full controlled access highway | Conestoga Parkway | Highway 7 | Waterloo Regional Road 85 | Kitchener, Waterloo | Part of the Conestoga Parkway. Becomes Waterloo Regional Road 85 near Waterloo city limits. Only highway entirely within the region. | - | - | - | - | - | - | - |
| Full controlled access highway | | Waterloo Regional Road 70 | Waterloo Regional Road 33 | Kitchener, Cambridge | | - | - | - | - | - | - | - |