Virtual-'Light-Sheet' Single-Molecule Localisation Microscopy Enables Quantitative Optical Sectioning for Super-Resolution Imaging


Virtual-'Light-Sheet' Single-Molecule Localisation Microscopy Enables Quantitative Optical Sectioning for Super-Resolution Imaging is a scholarly work by Thomas J Etheridge, Matthieu Palayret, Srinjan Basu, Ulrike Endesfelder, David Lando, Mike Heilemann, Ernest D. Laue, Tony Carr, and David Klenerman, published in 2015 in ''PLOS One''. The main subjects of the publication include materials science, optics, resolution, super-resolution microscopy, microscope, optical microscope, Optical sectioning, diffraction, Light sheet fluorescence microscopy, photoactivated localization microscopy, microscopy, fluorescence microscope, super-resolution imaging, Bioimage informatics, and cryogenic electron microscopy. The authors describe an easy-to-use routine (implemented as an open-source ImageJ plug-in) to quickly analyse a calibration sample to define and use such a virtual light-sheet.