Virginia State Route 727
State Route 727 in the U.S. state of Virginia is a secondary route designation applied to multiple discontinuous road segments among the many counties. The list below describes the sections in each county that are designated SR 727.
List
| County | Length | Length | From | Via | To | Notes |
| Accomack | SR 606 | Harbor Circle | Dead End | - | ||
| Albemarle | SR 795 | Blenheim Road | SR 627 | - | ||
| Amherst | SR 130 | Cheatham Road Pera Road | SR 130 | - | ||
| Augusta | SR 731 | Wolf Ridge Road Grindstone Road Millers Sawmill Road | Rockingham County line | Gap between segments ending at different points along SR 730 | - | |
| Bedford | SR 626 | Crab Orchard Road | SR 24 | - | ||
| Botetourt | US 220 | Fork Farm Road | Alleghany County line | - | ||
| Campbell | Dead End | Crows Road | SR 701 | - | ||
| Carroll | Dead End | Farmville Lane Old Quaker Road | SR 815 | Gap between segments ending at different points along SR 97 | - | |
| Chesterfield | SR 868 | Murray Olds Drive Alverser Drive | SR 147 | - | ||
| Dinwiddie | Dead End | Bethune Road | SR 675 | - | ||
| Fairfax | Cul-de-Sac | Fountainhead Regional Park Road | SR 647 | - | ||
| Fauquier | Dead End | Hard Scrabble Road | SR 726 | - | ||
| Franklin | Dead End | Riverbrook Road | Henry County line | - | ||
| Frederick | US 11 | Belle Grove Road | Dead End | - | ||
| Halifax | Dead End | North Nichols Ferry Trail | SR 716 | - | ||
| Hanover | Dead End | Holly Berry Road | SR 623 | - | ||
| Henry | Franklin County line | Stoneybrook Lane | SR 674 | - | ||
| James City | Dead End | Oxford Road | SR 31 | - | ||
| Loudoun | SR 722 | Forest Mills Road | SR 729 | - | ||
| Louisa | Dead End | Mickie Town Road | SR 635 | - | ||
| Mecklenburg | North Carolina state line | Henrico Road | SR 871 | Gap between segments ending at different points along SR 728 | - | |
| Montgomery | Dead End | Fox Hollow Road | SR 603 | - | ||
| Pittsylvania | SR 41 | Afton Road | SR 745 | - | ||
| Prince William | SR 234 | Olympic Drive | Dead End | - | ||
| Pulaski | SR 670 | Hodge Hollow Road | Dead End | - | ||
| Roanoke | SR 622 | Berrybrook Drive | Dead End | - | ||
| Rockbridge | SR 602 | Unnamed road | SR 252 | Gap between dead ends Gap between segments ending at different points along SR 39 Gap between segments ending at different points along SR 712 | - | |
| Rockingham | Augusta County line | Sangersville Road Spring Creek Road Airport Road | US 11 | Gap between segments ending at different points along SR 613 Gap between segments ending at different points along SR 42 | - | |
| Scott | US 23 | Unnamed road | US 23 | - | ||
| Shenandoah | SR 42 | Solomons Church Road | SR 716 | - | ||
| Spotsylvania | Dead End | Rosser Street | US 17 Bus/SR 2 | - | ||
| Stafford | Dead End | Monroe Farm Road | SR 655 | - | ||
| Tazewell | Bland County line | West End Road | SR 623 | - | ||
| Washington | Tennessee state line | Dry Branch Road | SR 726 | - | ||
| Wise | SR 644 | Unnamed road | Dead End | - | ||
| York | Dead End | Nelson Drive | SR 752 | - |