Super-Resolution Microscopy Using Standard Fluorescent Proteins in Intact Cells under Cryo-Conditions
Super-Resolution Microscopy Using Standard Fluorescent Proteins in Intact Cells under Cryo-Conditions is a scholarly work by Yvonne Jones, Ilan Davis, Kay Grünewald, Elena Seiradake, Ian M. Dobbie, and Christoph Hagen, published in 2014 in ''Nano Letters''. The main subjects of the publication include fluorescence microscope, liquid nitrogen, photobleaching, resolution, biophysics, Fluorescence-lifetime imaging microscopy, optical microscope, fluorescence, super-resolution microscopy, fluorescence recovery after photobleaching, photoactivated localization microscopy, chemistry, materials science, cryogenic electron microscopy, nanotechnology, molecule, microscopy, digital holographic microscopy, and microscope. The authors introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K).