Spot-tag
A Spot-tag is a 12-amino acid peptide tag recognized by a single-domain antibody. Due to the small size of a Spot-tag and the robust Spot-nanobody that specifically binds to Spot-tagged proteins, Spot-tag can be used for multiple capture and detection applications: Immunoprecipitation, affinity purification, immunofluorescence, and super-resolution microscopy. Recombinant proteins can be engineered to express the Spot-tag.
Spot-tag Sequence
Amino acid sequence
PDRVRAVSHWSSCodon optimized DNA sequence
| Human | CCA GAC CGC GTG CGC GCC GTG AGC CAT TGG AGC AGC |
| S. cerevisiae | CCA GAT AGA GTT AGA GCT GTT TCT CAT TGG TCT TCT |
| E. coli | CCG GAT CGC GTG CGC GCA GTC TCT CAC TGG AGC AGC |