| Name | Symbols | Chartered | Founding location | Type | Scope | Emphasis | Status | Reference |
| Alpha Lambda Zeta | ΑΛΖ | 2006 | Houston, Texas and Atlanta, Georgia | Women's service fraternity; non-collegiate | National | masculine-identifying lesbians | Active | |
| Alpha Pi Delta | ΑΠΔ | | Dallas, Texas | Service | National | lesbians of color | Active | |
| Beta Phi Omega | ΒΦΩ | 2000 | Tallahassee Florida | Sorority | National | feminine lesbian or bi-sexual of color | Active | |
| Eta Epsilon Gamma | ΗΕΓ | 2009 | Jackson, Mississippi | Social sorority | Local | Females at birth who identifies as cis-gender bisexual or lesbians | Active | |
| Eta Iota Mu | ΗΙΜ | 2008 | Jackson, Mississippi | Social women's fraternity | Local | Masculine identified cis-gender women and openly transgender males | Active | |
| Kappa Alpha Lambda | ΚΑΛ | 2003 | Clark Atlanta University | Service sorority, non-collegiate | National | feminine lesbian | Active | |
| Kappa Omega Omicron | ΚΏΟ | 2009 | Southern California | Women's professional fraternity, non-collegiate | National | masculine identified women and trans men | Active | |
| Kappa Theta Epsilon | ΚΘΕ | 2009 | Houston, Texas | Professional and entrepreneur sorority | National | Lesbian | Active | |
| Kappa Xi Omega | ΚΞΩ | 2004 | Florida | Service sorority; non-colliegiate | National | professional lesbians | Active | |
| Lambda Delta Lambda | ΛΔΛ | 1988 | University of California, Davis | LGBTQIA Sorority | National | queer, gay, lesbians, bisexuals, and all genders and sexualities | Active | |
| Omicron Epsilon Pi | ΟΕΠ | 2000 | Tallahassee, Florida | Sorority, non-collegiate | National | lesbians, particularly those of color | Active | |
| Sigma Omega Phi | ΣΩΦ | 2008 | Atlanta, Georgia | Women's service fraternity | National | masculine-identified lesbian | Active | |
| ΖΘΨ | 2013 | Heidelberg University | Sorority | Local | female identifying students with non-gendered corollary | Active | |
| Name | Symbols | Chartered | Formation location | Type | Scope | Emphasis | Status | References |
| Beta Gamma Chi | ΒΓΠ | 2009 | Lincoln University (Pennsylvania) | LGBTQ fraternity, non-collegiate | National | queer-affirming siblinghood | Active | |
| Delta Lambda Phi | ΔΛΦ | 1986 | Washington, D.C; | Social fraternity, collegiate and non-collegiate | International | gay, bisexual, transgender, and progressive men | Active | |
| Delta Phi Upsilon | ΔΦΥ | 1995 | Houston, Texas | Social fraternity, non-colligiate | National | gay men of color | Active | |
| Kappa Psi Kappa | ΚΨΚ | 2001 | Tallahassee, Florida | Fraternity; non collegiate | National | progressive men of all sexual orientations | Active | |
| Sigma Epsilon Omega | ΣΕΩ | 2007 | University of California, Berkeley | Gay and queer fraternity | Local | all genders | Active | |
| Sigma Phi Beta | ΣΦΒ | 2003 | Arizona State University | Society fraternity | National | All who identify as male, gay, straight, bisexual, and transgender men | Active | |
| Name | Symbol | Chartered | Founding location | Type | Scope | Policy | Reference |
| Alpha Chi Omega | ΑΧΩ | 1885 | DePauw University | Women's fraternity, social | International | open to any individual who identifies as a woman and trans woman, any sexual preference | |
| Alpha Delta Pi | ΑΔΠ | 1851 | Wesleyan College | Sorority, social | International | open to any individual who identifies as a woman, including trans women | |
| Alpha Epsilon Phi | ΑΕΦ | 1909 | Barnard College | Sorority, Jewish social | National | open to any individual who identifies as a woman, regardless of the gender assigned at birth | |
| Alpha Gamma Delta | ΑΓΔ | 1904 | Syracuse University | Sorority, social | International | open to women of any sexual orientation and any individual who identifies as a woman | |
| Alpha Omicron Pi | ΑΟΠ | 1897 | Barnard College | Sorority, social | International | open to any individual who identifies and lives as a woman with any sexual orientation | |
| Alpha Sigma Tau | ΑΣΤ | 1899 | Eastern Michigan University | Sorority, social | National | open to women and transgender persons who identify as women with any sexual orientation | |
| Alpha Xi Delta | ΑΞΔ | 1893 | Lombard College | Sorority, social | National | open to any individual who identifies and lives as a woman with any sexual orientation | |
| Acacia | | 1904 | University of Michigan | Fraternity, social | International | inclusive of gender identity or expression or sexual orientation | |
| Delta Delta Delta | ΔΔΔ | 1888 | Boston University | Sorority | International | open to any individual who identifies and lives as a woman or any sexual orientation | |
| Delta Gamma | ΔΓ | 1873 | Lewis School for Girls | Women's fraternity, social | International | open to any individual who identifies as a woman and any sexual orientation | |
| Delta Phi Epsilon | ΔΦΕ | 1917 | New York University Law School | Sorority | International | explicitly open to trans and non-binary individuals | |
| Delta Tau Delta | ΔΤΔ | 1858 | Bethany College | Fraternity, social | International | explicitly inclusive of individuals who identify as "gay, bisexual-transgender and any other non-heteronormative sexual orientations, gender expression, or genre identities" | |
| Delta Upsilon | ΔΥ | 1834 | Williams College | Fraternity | International | explicitly welcomes any individuals who identify as male, transgender men, and men of any sexual orientation | |
| Gamma Phi Beta | ΓΦΒ | 1874 | Syracuse University | Sorority | International | open to any individual who identifies as a woman | |
| Kappa Alpha Theta | ΚΑΘ | 1870 | DePauw University | Sorority | International | open to any individual who identifies as a woman | |
| Kappa Delta | ΚΔ | 1897 | Longwood University | Sorority | National | open to any individual who identifies as a woman and women of any sexual orientation | |
| Kappa Kappa Gamma | ΚΚΓ | 1870 | Monmouth College | Sorority | International | open to any individual who identifies as a woman and women of any sexual orientation | |
| Omega Delta Phi | ΩΔΦ | 1987 | Texas Tech University | Fraternity, multicultural | National | inclusive of all men, regardless of assigned sex at birth | |
| Phi Omega | ΦΩ | 1962 | Cornell University | Sorority, social | National | female-identifying and non-binary | |
| Phi Sigma Sigma | ΦΣΣ | 1913 | Hunter College | Sorority | International | open to any individual who identifies as a woman with any sexual orientation | |
| Pi Lambda Phi | ΠΛΦ | 1895 | Yale University | Fraternity | International | explicitly inclusive of gay and transgender men | |
| Sigma Chi | ΣΧ | 1855 | Miami University | Fraternity, literary and social | International | open to men of any sexual orientation | |
| Sigma Delta Tau | ΣΔΤ | 1917 | Miami University | Sorority, social | International | open to any who identify and live as a woman of any sexual orientation | |
| Sigma Phi Chi | ΣΦΧ | 2001 | Austin College | Sorority, Christian and social | Local | inclusive and LGBTQ+ friendly | |
| Sigma Sigma Sigma | ΣΣΣ | 1898 | Longwood University | Sorority, social | International | open to any individual who identifies as a woman | |
| Tau Kappa Epsilon | ΤΚΕ | 1899 | Illinois Wesleyan University | Fraternity, social | International | explicitly inclusive of gay and transgender men | |
| Theta Phi Alpha | ΘΦΑ | 1912 | University of Michigan | Sorority, social | National | explicitly open to trans women and any individual who identifies as a woman | |
| Zeta Alpha Delta | ΖΑΔ | 2007 | Oxford, Mississippi | Fraternity, non-collegiate | National | inclusive men of all sexual orientations, male-identified individuals | |
| Zeta Omega Eta | ΖΩΗ | 2003 | Trinity College | Sorority, coed feminist | Regional | inclusive and LGBTQ+ friendly | |
| Zeta Tau Alpha | ΖΤΑ | 1898 | Longwood University | Sorority | International | open to individuals who consistently identify and live as a woman of any sexual orientation | |