Scottish National League Division One
The Scottish National League Division One is the second tier of the Scottish League Championship for amateur rugby union clubs in Scotland.
The division was established in its current format in 2014, with the creation of three national leagues below the Premiership.
For the 2023–24 season, the division was reduced from 12 to 10 teams.
Promotion and Relegation
The winners are promoted to the [Scottish Scottish Premiership (rugby)|Premiership (rugby)|Premiership] while relegation is to National League Division 2.2025–26 Teams
Departing were GHA, promoted to the Scottish Premiership while Highland, Dundee and Peebles were relegated to National League Division 2.Promoted from 2024–25 National League Division 2
Relegated from 2024–25 Scottish Premiership
| Club | Location | Home Ground |
| Biggar | Biggar | Hartree Mill |
| Boroughmuir | Edinburgh | Meggetland |
| Edinburgh Academical | Edinburgh | Raeburn Place |
| Gala | Galashiels | Netherdale |
| Glasgow Academicals | Glasgow | New Anniesland |
| Gordonians | Aberdeen | Countesswells |
| Jed-Forest | Jedburgh | Riverside Park |
| Marr | Troon | Fullarton Park |
| Musselburgh | Musselburgh | Stoneyhill |
| Stirling County | Stirling | Bridgehaugh Park |
Past winners
Winners of the second tier competition – includes National League Division Two, Premiership Division Two, National League and National League Division OneNational League Division Two
- Kelso
- Langholm
- Selkirk
- Melrose
- Kelso
- Melrose
- Gordonians
- Selkirk
- Kilmarnock
- Ayr
- Glasgow Academicals
- Kilmarnock
- Glasgow Academicals
- Kilmarnock
- Jed-Forest
- Stirling County
- Watsonians
- Kelso
- West Of Scotland
- Glasgow High Kelvinside
- Kelso
- Currie
- Glasgow Hawks
- Gala
- Boroughmuir
- Stirling County
- Peebles
- Watsonians
- Gala
- Stirling County
- Dundee HSFP
- Stirling County
- West Of Scotland
- Dundee HSFP
- Stirling County
- Edinburgh Academical '
- Jed-Forest
- Glasgow Hawks
- Boroughmuir
- Selkirk
- Watsonians
- Marr
- Edinburgh Academical
- Marr