List of highways numbered 250
- Arkansas Highway 250
- California State Route 250
- Farm to Market Road 250
- Georgia State Route 250
- Hawaii Route 250
- Illinois Route 250
- Indiana State Road 250
- Japan National Route 250
- Kentucky Route 250
- Manitoba Provincial Road 250
- Minnesota State Highway 250
- National Route 250 (Costa Rica)
- Nebraska Highway 250
- New Mexico State Road 250
- New York State Route 250
- Oregon Route 250
- Pennsylvania Route 250
- Prince Edward Island Route 250
- Puerto Rico Highway 250
- Tennessee State Route 250
- Texas State Highway 250
- Texas State Highway Loop 250
- U.S. Route 250
- Utah State Route 250