Pisano was born in Naples, into a family of dialect theatre actors. She debuted on stage as a child actress in her family company, and later entered the stage company of Eduardo and Peppino De Filippo. After the war and after the dissolution of the company she continued working with Eduardo De Filippo, and starting from the 1950s with other companies, notably the one held by Nino Taranto. Pisano was also active in films and on television, even if mainly cast in supporting roles.